A5045071454- Metal-Catalyzed Oxygenation Mechanisms
- RNA modifications and cancer
- Ferroptosis and cancer prognosis
- Cancer-related molecular mechanisms research
- Genetic Mapping and Diversity in Plants and Animals
- Genetic and phenotypic traits in livestock
- Animal Genetics and Reproduction
- Cancer Immunotherapy and Biomarkers
- Metalloenzymes and iron-sulfur proteins
- MicroRNA in disease regulation
- Microbial Natural Products and Biosynthesis
- RNA Research and Splicing
- Circular RNAs in diseases
- RNA regulation and disease
- Epigenetics and DNA Methylation
- Photosynthetic Processes and Mechanisms
- Cancer-related gene regulation
- Cancer Genomics and Diagnostics
- Chemical Synthesis and Analysis
- Ocular Diseases and Behçet’s Syndrome
- Cancer Cells and Metastasis
- Pickering emulsions and particle stabilization
- Synthesis and Catalytic Reactions
- Molecular Biology Techniques and Applications
- Surfactants and Colloidal Systems
Ningxia Medical University
2025
Ningxia Medical University General Hospital
2025
Chinese Academy of Agricultural Sciences
2019-2024
Affiliated Hospital of Nantong University
2020-2024
Nantong University
2019-2024
Agricultural Genomics Institute at Shenzhen
2019-2024
Carnegie Mellon University
2018-2024
Tongji University
2024
Qingdao University
2024
Affiliated Hospital of Qingdao University
2024
A high voltage (1.38 V) total organic aqueous redox flow battery is reported using 1,1'-bis[3-(trimethylammonio)propyl]-4,4'-bipyridinium tetrachloride ((NPr)2V) as an anolyte and 4-trimethylammonium-TEMPO chloride (NMe-TEMPO) a catholyte. The exceptional radical stability of [(NPr)2V]+˙ enabled the in achieving 97.48% capacity retention for 500 cycles power density 128.2 mW cm-2.
Abstract DNA methylation is important for the epigenetic regulation of gene expression and plays a critical role in mammalian development. However, dynamic genome-wide skeletal muscle development remains largely unknown. Here, we generated first single-base resolution methylome transcriptome maps porcine across 27 developmental stages. The overall level decreased from embryo to adult, which was highly correlated with downregulated DNMT1 an increase partially methylated domains. Notably,...
Mechanisms of enzymatic epoxidation via oxygen atom transfer (OAT) to an olefin moiety is mainly derived from the studies on thiolate-heme containing epoxidases, such as cytochrome P450 epoxidases. The molecular basis catalyzed by nonheme-iron enzymes much less explored. Herein, we present a detailed study nonheme iron(II)- and 2-oxoglutarate-dependent (Fe/2OG) oxygenase, AsqJ. native substrate analogues with different para substituents ranging electron-donating groups (e.g., methoxy)...
Abstract Background Circular RNAs (circRNAs) represent a novel class of non‐coding formed by covalently closed loop and play crucial roles in many biological processes. Several circRNAs associated with myogenesis have been reported. However, the dynamic expression, function, mechanism during skeletal muscle development are largely unknown. Methods Strand‐specific RNA‐sequencing (RNA‐seq) microarray datasets were used to profile circRNAome landscape myogenic differentiation. Bioinformatics...
The genetic mechanisms that underlie phenotypic differentiation in breeding animals have important implications evolutionary biology and agriculture. However, the contribution of cis-regulatory variants to pig phenotypes is poorly understood. Therefore, our aim was elucidate molecular by which non-coding cause differences pigs combining analyses functional genomics.We obtained a high-resolution phased chromosome-scale reference genome with contig N50 18.03 Mb for Luchuan breed (a...
Porcine reproductive and respiratory syndrome (PRRS) is an economically important disease of swine that caused by PRRS virus (PRRSV). In this study, we established a fluorescence assay for highly sensitive detection PRRSV through integration the reverse transcription–recombinase polymerase amplification (RT-RPA)-coupled Cas12a system with optical property single stranded DNA–fluorescently quenched (ssDNA-FQ) reporter. This technique can achieve isothermal visual in 25 min. particular,...
Abstract Background Cuproptosis-related genes (CRGs) are associated with lung adenocarcinoma. However, the links between CRGs and non-small-cell cancer (NSCLC) not clear. In this study, we aimed to develop two cuproptosis models investigate their correlation NSCLC in terms of clinical features tumor microenvironment. Methods CRG expression profiles data from normal tissues was obtained GEO (GSE42127) TCGA datasets. Molecular clusters were classified into three patterns based on...
Objective: To investigate the clinical features and prognosis of neuro-Behçet's syndrome (NBS) in children. Method: The clinical, brain magnetic resonance imaging laboratory data 5 children with NBS diagnosed Department Pediatrics, General Hospital Ningxia Medical University Rheumatology Immunology, Children's Affiliated to Capital Institute Pediatrics from April 2014 2024 were analyzed retrospectively. follow-up method was retrospective outpatient or inpatient visit evaluate treatment...
Abstract Dynamically engineering the interfacial interaction of nanoparticles has emerged as a new approach for bottom‐up fabrication smart systems to tailor molecular diffusion and controlled release. Janus zwitterionic nanoplates are reported that can be switched between locked unlocked state at interfaces upon changing surface charge, allowing manipulation properties in fast, flexible, switchable manner. Combining experimental modeling studies, an unambiguous correlation is established...
Respiratory syncytial virus (RSV) and human metapneumovirus (HMPV) are two common viral pathogens in acute lower respiratory tract infections (ALRTI). However, the association of load with clinical characteristics is not well-defined ALRTI. To explore correlation between RSV HMPV children hospitalized for ALRTI Lanzhou, China. Three hundred eighty-seven were enrolled. Nasopharyngeal aspirates (NPAs) sampled from each children. Real-time PCR was used to screen RSV, HMPV, twelve additional...
Installation of olefins into molecules is a key transformation in organic synthesis. The recently discovered decarboxylation-assisted olefination the biosynthesis rhabduscin by mononuclear non-heme iron enzyme (P.IsnB) represents novel approach olefin construction. This method commonly employed natural product biosynthesis. Herein, we demonstrate that ferryl intermediate used for C–H activation at benzylic position substrate. We further establish P.IsnB reactivity can be switched from to...
Abstract Background Sleep deprivation (SD) often leads to complex detrimental consequences, though the mechanisms underlying these dysfunctional effects remain largely unknown. We investigated whether right stellate ganglion block in rats can improve spatial learning and memory dysfunction induced by sleep alleviating damage of hippocampus rats. Methods Sixty four male Sprague Dawley were randomly divided into groups: Control, SD (sleep deprivation), SGB (stellate block) + block+...
Despite the diversity of reactions catalyzed by 2-oxoglutarate-dependent nonheme iron (Fe/2OG) enzymes identified in recent years, only a limited number these have been investigated mechanistic detail. In particular, several Fe/2OG-dependent capable catalyzing isocyanide formation reported. While glycine moiety has as biosynthon for group, how actual conversion is effected remains obscure. To elucidate catalytic mechanism, we characterized two previously unidentified (AecA and AmcA) along...
Mononuclear non-heme iron enzymes are a large class of catalyzing wide-range reactions. In this work, we report that enzyme in Methyloversatilis thermotolerans, OvoAMtht, has two different activities, as thiol oxygenase and sulfoxide synthase. When cysteine is presented the only substrate, OvoAMtht oxygenase. presence both histidine substrates, catalyzes oxidative coupling between (a synthase). Additionally, demonstrate substrates active site iron's secondary coordination shell residues...
Ergothioneine (ESH) and ovothiol A (OSHA) are two natural thiol-histidine derivatives. ESH has been implicated as a longevity vitamin OSHA inhibits the proliferation of hepatocarcinoma. The key biosynthetic step in aerobic pathways is O
Two non-heme iron enzymes, IsnB and AmbI3, catalyze a novel decarboxylation-assisted olefination to produce indole vinyl isonitrile, an important building block for many natural products. Compared other reactions catalyzed by this enzyme family, represents attractive biosynthetic route mechanistically unexplored pathway in constructing C═C bond. Using mechanistic probes, transient state kinetics, reactive intermediate trapping, spectroscopic characterizations, product analysis, we propose...
N-alkylisonitrile, a precursor to isonitrile-containing lipopeptides, is biosynthesized by decarboxylation-assisted -N≡C group (isonitrile) formation using N-alkylglycine as the substrate. This reaction catalyzed iron(II) and 2-oxoglutarate (Fe/2OG) dependent enzymes. Distinct from typical oxygenation or halogenation reactions this class of enzymes, installation isonitrile represents novel type for Fe/2OG enzymes that involves four-electron oxidative process. Reported here plausible...
Abstract Nowadays, research on CSCs is still in an initial stage, and there are few studies reporting the successful isolation identification of CSCs. In present study, we attempted to isolate through cultivating cell line MKN45 defined serum‐free medium study expression stem markers or related proteins (Oct3/4, Sox2, Nanog CD44) Moreover, immunofluorescence staining was performed validate spheroid body‐forming cells. Further experiments were used evaluate SPOP tumorsphere addition,...
Circular RNAs (circRNAs) represent a class of covalently closed single-stranded RNA molecules that are emerging as essential regulators various biological processes. The circRNA circHipk2 originates from exon 2 the Hipk2 gene in mice and was reported to be involved acute promyelocytic leukemia myocardial injury. However, functions mechanisms myogenesis largely unknown. Here, deepen our knowledge about role circHipk2, we studied expression function during skeletal myogenesis. We found mostly...
Adenosine-to-inosine (A-to-I) RNA editing meditated by adenosine deaminases acting on (ADARs) enzymes is a widespread post-transcriptional event in mammals. However, A-to-I skeletal muscle remains poorly understood. By integrating strand-specific RNA-seq, whole genome bisulphite sequencing, and sequencing data, we comprehensively profiled the editome developing muscles across 27 prenatal postnatal stages pig, an important farm animal biomedical model. We detected 198,892 sites found that...
Isonitrile group has been identified in many natural products. Due to the broad reactivity of N≡C triple bond, these products have valuable pharmaceutical potentials. This review summarizes current biosynthetic pathways and corresponding enzymes that are responsible for isonitrile-containing product generation. Based on strategies utilized, two fundamentally distinctive approaches discussed. In addition, recent progress elucidating isonitrile formation mechanisms is also presented.
Oral squamous cell carcinoma (OSCC) is one of the most prevalent cancers with poor prognosis in head and neck. Elucidating molecular mechanisms underlying OSCC occurrence development important for therapy. Dysregulated palmitoylation-related enzymes have been reported several but OSCC.
Abstract Background As a highly heterogeneous tumor, non-small cell lung cancer (NSCLC) is famous for its high incidence and mortality worldwide. Smoking can cause genetic changes, which leading to the occurrence progress of NSCLC. Nevertheless, function smoking-related genes in NSCLC needs more research. Methods We downloaded transcriptome data clinicopathological parameters from Gene Expression Omnibus (GEO) databases, screened genes. Lasso regression were applied establish 7-gene...