A5021107850- Electron Spin Resonance Studies
- Spectroscopy and Quantum Chemical Studies
- Photosynthetic Processes and Mechanisms
- Lanthanide and Transition Metal Complexes
- Molecular Junctions and Nanostructures
- Electrochemical Analysis and Applications
- Advanced NMR Techniques and Applications
- Genomics and Phylogenetic Studies
- Magnetism in coordination complexes
- Metal-Catalyzed Oxygenation Mechanisms
- Photochemistry and Electron Transfer Studies
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Microbial Community Ecology and Physiology
- Hemoglobin structure and function
- Advanced MRI Techniques and Applications
- Crystallography and molecular interactions
- Heme Oxygenase-1 and Carbon Monoxide
- Agricultural Productivity and Crop Improvement
- Protein Structure and Dynamics
- ATP Synthase and ATPases Research
- Analytical Chemistry and Sensors
- Lipid Membrane Structure and Behavior
- Photoreceptor and optogenetics research
- Biotin and Related Studies
North Carolina State University
2015-2024
Frederick National Laboratory for Cancer Research
2019-2022
National Cancer Institute
2019
Joint Genome Institute
2011-2018
Weatherford College
2017
Tver State Agricultural Academy
2012
Lawrence Berkeley National Laboratory
2011-2012
Bruker (United States)
2008
Argonne National Laboratory
2007
Institute of Cytology
2001
MycoCosm is a fungal genomics portal (http://jgi.doe.gov/fungi), developed by the US Department of Energy Joint Genome Institute to support integration, analysis and dissemination genome sequences other 'omics' data providing interactive web-based tools. also promotes facilitates user community participation through nomination new species fungi for sequencing, annotation resulting data. By efficiently filling gaps in Fungal Tree Life, will help address important problems associated with...
The Genomes OnLine Database (GOLD, http://www.genomesonline.org/) is a comprehensive resource for centralized monitoring of genome and metagenome projects worldwide. Both complete ongoing projects, along with their associated metadata, can be accessed in GOLD through precomputed tables search page. As September 2011, GOLD, now on version 4.0, contains information 11,472 sequencing which 2907 have been completed sequence data has deposited public repository. Out these 1918 are finished 989...
The Integrated Microbial Genomes & Microbiomes system v.5.0 (IMG/M: https://img.jgi.doe.gov/m/) contains annotated datasets categorized into: archaea, bacteria, eukarya, plasmids, viruses, genome fragments, metagenomes, cell enrichments, single particle sorts, and metatranscriptomes. Source include those generated by the DOE's Joint Genome Institute (JGI), submitted external scientists, or collected from public sequence data archives such as NCBI. All submissions are typically processed...
The U.S. Department of Energy (DOE) Joint Genome Institute (JGI), a national user facility, serves the diverse scientific community by providing integrated high-throughput sequencing and computational analysis to enable system-based approaches in support DOE missions related clean energy generation environmental characterization. JGI Portal (http://genome.jgi.doe.gov) provides unified access all genomic databases analytical tools. maintains extensive data management systems specialized...
The Department of Energy (DOE) Joint Genome Institute (JGI) is a national user facility with massive-scale DNA sequencing and analysis capabilities dedicated to advancing genomics for bioenergy environmental applications. Beyond generating tens trillions bases annually, the develops maintains data management systems specialized analytical manage interpret complex genomic sets, enable an expanding community users around world analyze these in different contexts over web. JGI Portal...
Emerging applications of nanosized iron oxides in nanotechnology introduce vast quantities nanomaterials into the human environment, thus raising some concerns. Here we report that surface γ-Fe2O3 nanoparticles 20−40 nm diameter mediates production highly reactive hydroxyl radicals (OH•) under conditions biologically relevant superoxide-driven Fenton reaction. By conducting comparative spin-trapping EPR experiments, show free radical is attributed primarily to catalytic reactions at...
The Genomes OnLine Database (GOLD, http://www.genomesonline.org/) is a comprehensive resource for centralized monitoring of genome and metagenome projects worldwide. Both complete ongoing projects, along with their associated metadata, can be accessed in GOLD through precomputed tables search page. As September 2011, GOLD, now on version 4.0, contains information 11,472 sequencing which 2907 have been completed sequence data has deposited public repository. Out these 1918 are finished 989...
Age-related macular degeneration (AMD) is the foremost cause of irreversible blindness in people over age 65 especially developing countries. Therefore, an exploration effective and alternative therapeutic interventions unmet medical need. It has been established that oxidative stress plays a key role pathogenesis AMD, hence, neutralizing strategy for treatment this serious disorder. Owing to autoregenerative properties, nanoceria widely used as nonenzymatic antioxidant related disorders....
Cyclic voltammetry is a widely used and powerful tool for sensitively selectively measuring hydrogen peroxide (H2O2). Herein, was combined with electron paramagnetic resonance spectroscopy to identify define the role of an oxygen-centered radical liberated during oxidation H2O2. The spin-trap reagents, 5,5-dimethyl-1-pyrroline N-oxide (DMPO) 2-ethoxycarbonyl-2-methyl-3,4-dihydro-2H-pyrrole-1-oxide (EMPO), were employed. Spectra exhibit distinct hyperfine patterns that clearly DMPO(•)-OH...
Abstract Plasma-generated hydroxyl radicals ( · OH) and oxygen atoms (O) produced by the COST reference plasma jet, a micro-scaled atmospheric pressure were investigated using variety of experimental techniques. Several gas admixtures studied to distinguish contributions two reactive species. Large discrepancies between inferred aqueous OH densities noted when 2-hydroxyterephthalic acid (HTA) fluorescence assay electron paramagnetic resonance (EPR) measurements with spin trap...
Abstract Background The cancer genome is commonly altered with thousands of structural rearrangements including insertions, deletions, translocation, inversions, duplications, and copy number variations. Thus, variant (SV) characterization plays a paramount role in target identification, oncology diagnostics, personalized medicine. As part the SEQC2 Consortium effort, present study established evaluated consensus SV call set using breast reference cell line matched normal control derived...
Dehaloperoxidase (DHP), the oxygen transport hemoglobin from terebellid polychaete Amphitrite ornata, is first globin identified to possess a biologically relevant peroxidase activity. DHP has been shown oxidize trihalophenols dihaloquinones in dehalogenation reaction that uses hydrogen peroxide as substrate. Herein, we demonstrate detectable intermediate following addition of ferric contains both ferryl heme and tyrosyl radical, analogous Compound ES cytochrome c peroxidase. Furthermore,...
Dehaloperoxidase (DHP) from the terebellid polychaete Amphitrite ornata is a bifunctional enzyme that possesses both hemoglobin and peroxidase activities. Of two DHP isoenzymes identified to date, much of recent focus has been on A, whereas very little known pertaining activity, substrate specificity, mechanism function, or spectroscopic properties B. Herein, we report recombinant expression purification B, as well details our investigations into its catalytic cycle using biochemical assays,...
Installation of olefins into molecules is a key transformation in organic synthesis. The recently discovered decarboxylation-assisted olefination the biosynthesis rhabduscin by mononuclear non-heme iron enzyme (P.IsnB) represents novel approach olefin construction. This method commonly employed natural product biosynthesis. Herein, we demonstrate that ferryl intermediate used for C–H activation at benzylic position substrate. We further establish P.IsnB reactivity can be switched from to...
Chlamydia trachomatis (Ct) infections are the most common sexually transmitted infection (STI). Despite effective antibiotics for Ct, undetected or delayed treatment can lead to infertility, ectopic pregnancies, and chronic pelvic pain. Besides humans, chlamydia poses similar health challenges in animals such as C. suis (Cs) pigs. Based on similarities between humans pigs, well their species, we use pigs a large biomedical animal model research. In this study, used pig develop vaccine...
The structures, optical bandgap sizes, and photocatalytic activities are described for three copper-octamolybdate hybrid solids prepared using hydrothermal methods, [Cu(pda)]4[β-Mo8O26] (I; pda = pyridazine), [Cu(en)2]2[γ-Mo8O26] (II; en ethylenediamine), [Cu(o-phen)2]2[α-Mo8O26] (III; o-phen o-phenanthroline). structure of I consists a [Cu(pda)]4(4+) tetramer that bridges to neighboring [β-Mo8O26](4-) octamolybdate clusters form two-dimensional layers stack along the axis. previously...
IspH is a 4Fe-4S protein that carries out an essential reduction step in isoprenoid biosynthesis. Using hyperfine sublevel correlation (HYSCORE) spectroscopy, we show pyridine inhibitors of directly bind to the unique fourth Fe cluster, opening up new routes inhibitor design, interest context both anti-bacterial as well anti-malarial drug discovery.
Sec14-superfamily proteins integrate the lipid metabolome with phosphoinositide synthesis and signaling via primed presentation of phosphatidylinositol (PtdIns) to PtdIns kinases. Sec14 action as a PtdIns-presentation scaffold requires heterotypic exchange phosphatidylcholine (PtdCho) for PtdIns, or vice versa, in poorly understood progression regulated conformational transitions. We identify mutations that confer Sec14-like activities functionally inert pseudo-Sec14 (Sfh1), which seemingly...
The existence of radicals in pyrolysis oil generated from loblolly pine three different reactor systems was verified with electron paramagnetic resonance (EPR) spectroscopy.
IspG is a 4Fe–4S protein that carries out an essential reduction step in isoprenoid biosynthesis. Using electron–nuclear double resonance (ENDOR) and hyperfine sublevel correlation (HYSCORE) spectroscopies on labeled samples, we have specifically assigned the interactions reaction intermediate. These results help clarify nature of intermediate, supporting direct interaction between unique fourth Fe cluster C2 O3 ligand.