A5063073026- Genomics and Phylogenetic Studies
- Cancer Genomics and Diagnostics
- Genomics and Rare Diseases
- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- Oral microbiology and periodontitis research
- Gut microbiota and health
- Diatoms and Algae Research
- Molecular Biology Techniques and Applications
- Chromosomal and Genetic Variations
- RNA and protein synthesis mechanisms
- Streptococcal Infections and Treatments
- Infective Endocarditis Diagnosis and Management
- Microbial Community Ecology and Physiology
- Marine and coastal ecosystems
Illumina (United States)
2021-2024
University of Washington
2012
Abstract As CRISPR-based therapies enter the clinic, evaluation of safety remains a critical and active area study. Here, we employ clinical next generation sequencing (NGS) workflow to achieve high depth detect ultra-low frequency variants across exons genes associated with cancer, all exons, genome wide. In three separate primary human hematopoietic stem progenitor cell (HSPC) donors assessed in technical triplicates, electroporated high-fidelity Cas9 protein targeted loci (AAVS1, HBB ,...
Abstract Background The cancer genome is commonly altered with thousands of structural rearrangements including insertions, deletions, translocation, inversions, duplications, and copy number variations. Thus, variant (SV) characterization plays a paramount role in target identification, oncology diagnostics, personalized medicine. As part the SEQC2 Consortium effort, present study established evaluated consensus SV call set using breast reference cell line matched normal control derived...
A microcosm study in iron‐limited waters of the northeast subarctic Pacific Ocean was conducted to examine how iron availability affects frustule‐related response individual diatoms and thus total quantity silica precipitated by community. New per cell estimated using fluorescent stain 2‐(4‐pyridyl)‐5{[4‐dimethylaminoethyl‐aminocarbamoyl)‐methoxy]phenyl}oxazole (PDMPO). Differences new precipitation within a particular genus before after enrichment were small compared differences among...
The identification and classification of the non-haemolytic or viridans group streptococci have long been recognised as difficult unsatisfactory. Phenotypic genotypic heterogeneity resulted in ambiguous speciation, particularly with mutans other oral streptococci. This study was done to determine whether random amplified polymorphic DNA (RAPD) analysis is useful identify even classify prepared purified from 25 strains including 11 reference Streptococcus mutans, seven S. sobrinus, three...
Abstract Metagenomics enables direct investigation of the gene content and potential functions gut bacteria without isolation culture. However, metagenome-assembled genomes are often incomplete have low contiguity due to challenges in assembling repeated genomic elements. Long-read sequencing approaches successfully yielded circular bacterial directly from metagenomes, but these throughput, high DNA input requirements, can error rates. Illumina has recently launched Complete Long Read (ICLR)...
Abstract As CRISPR-based therapies enter the clinic, evaluation of safety remains a critical and still active area study. While whole genome sequencing is an unbiased method for identifying somatic mutations introduced by ex vivo culture editing, this methodology unable to attain sufficient read depth detect extremely low frequency events that could result in clonal expansion. solution, we utilized exon capture panel facilitate ultra-deep >500 tumor suppressors oncogenes most frequently...