A5039378442- American Constitutional Law and Politics
- Cancer Genomics and Diagnostics
- TGF-β signaling in diseases
- Single-cell and spatial transcriptomics
- Developmental Biology and Gene Regulation
- Phosphodiesterase function and regulation
- Extracellular vesicles in disease
- Genomics and Phylogenetic Studies
- Connective Tissue Growth Factor Research
- Wnt/β-catenin signaling in development and cancer
- Race, History, and American Society
- Political Science Research and Education
- Advanced Proteomics Techniques and Applications
- Chemical Synthesis and Analysis
- International Relations and Foreign Policy
- Genomics and Rare Diseases
- Cancer-related gene regulation
- Biomedical Ethics and Regulation
- 3D Printing in Biomedical Research
- Academic Freedom and Politics
- Pluripotent Stem Cells Research
- Molecular Biology Techniques and Applications
- Biochemical and Molecular Research
- Bone Metabolism and Diseases
- Peptidase Inhibition and Analysis
Center for Biologics Evaluation and Research
2009-2023
United States Food and Drug Administration
2007-2023
National Institutes of Health
1989-1997
Columbia University
1997
Paul Ehrlich Institut
1995
National Institute of Mental Health
1994
National Institute of Diabetes and Digestive and Kidney Diseases
1992-1993
National Heart Lung and Blood Institute
1992
National Cancer Institute
1990
University of Minnesota Medical Center
1988
Partially purified extracts from newborn calf articular cartilage were found to induce and bone when subcutaneously implanted in rats. This activity showed characteristics of morphogenetic proteins (BMPs). Degenerate oligonucleotide primer sets derived the highly conserved carboxyl-terminal region BMP family designed used reverse transcription-polymerase chain reactions with poly(A)+ RNA as template determine which BMPs are produced by chondrocytes. Two novel members transforming growth...
Hormone-sensitive lipase activity (HSL), which is found in the supernatant of centrifuged homogenates lipolytically quiet isolated rat adipocytes, was greatly reduced or absent from stimulated cells. The purified 100- to 250-fold cells 10-20% purity by a single passage over phenyl-Sepharose resin with high (greater than 70%) yields. Western blotting adipocyte homogenate fractions polyclonal antiserum raised against HSL showed that enzyme shifted quantitatively control floating "fat cake" A...
A method allowing initial sequencing yields of 60-86% to be consistently obtained from samples prepared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electrophoretic transfer is described in detail.Conducting at a pH near neutrality the single most important modifications made earlier procedures, but pre-electrophoresis presence glutathione or thioglycolate use ImmobilonTM polyvinylidene difluoride membranes all contribute success technique.When tryptophan was NH2 terminus...
ABSTRACT The mammalian zona pellucida is an extracellular matrix that surrounds growing oocytes, ovulated eggs and early embryos. mouse composed of three sulfated glycoproteins: ZP1, ZP2 ZP3. Each critically involved in fertilization, the postfertilization block to polyspermy protection preimplantation embryo. We have previously isolated cDNAs encoding ZP3 now report isolation a full-length cDNA ZP1. Mouse ZP1 623 amino acid polypeptide chain with signal peptide carboxyl terminal...
Convincing evidence has accumulated to identify the Frizzled proteins as receptors for Wnt growth factors. In parallel, a number of secreted frizzled-like with conserved N-terminal frizzled motif have been identified. One these proteins, Frzb-1, binds Wnt-1 and Xwnt-8 antagonizes signaling in Xenopus embryos. Here we report that Frzb-1 blocks induced cytosolic accumulation β-catenin, key component pathway, human embryonic kidney cells. Structure/function analysis reveals complete removal...
The major cAMP-dependent protein kinase (A-kinase) substrate in adipocytes is perilipin, a found exclusively at the surface of lipid storage droplets. Using anti-perilipin serum, we have isolated two related classes full-length coding cDNAs, designated perilipin A and B, from rat adipocyte cDNA expression library. cDNAs derive mRNA species that arise by differential splicing. mRNAs are predicted to encode perilipins proteins 517 aa (56,870 Da) 422 (46,420 Da), respectively, which share...
We have cloned a cDNA for myocardial cGMP-inhibited cAMP phosphodiesterase (cGI PDE) from human heart library in lambda Zap II. The open reading frame [3.5 kilobases (kb)] of clone n.13.2 (7.7 kb) encodes protein 125 kDa. In Northern blots total ventricle RNA, single mRNA species (8.3 hybridized with 4-kb EcoRI restriction fragment (containing the entire frame). carboxyl-terminal region deduced amino acid sequence cGI PDE contains putative catalytic domain conserved among mammalian families....
We have identified a novel growth factor in Xenopus, which is most closely related to human Bone Morphogenetic Protein-3. Its expression peaks during gastrulation, prominently the Spemann organizer, and persists posterior neural floor plate prechordal neurulation. Injection of corresponding mRNA into dorsal blastomeres results dose-dependent suppression anterior structures, even presence lithium chloride. Overexpression gene downregulates dorsalizing factors noggin, goosecoid follistatin, as...
A frog used for "hunting magic" by several groups of Panoan-speaking Indians in the borderline between Brazil and Peru is identified as Phyllomedusa bicolor. This frog's skin secretion, which introduce into body through fresh burns, rich peptides. These include vasoactive peptides, opioid a peptide that we have named adenoregulin, with sequence GLWSKIKEVGKEAAKAAAKAAGKAALGAVSEAV determined from mass spectrometry Edman degradation. The natural may contain D amino acid residue, since it not...
A cDNA which encodes a approximately 127 kDa UV-damaged DNA-binding (UV-DDB) protein with high affinity for (6-4)pyrimidine dimers [Abramic', M., Levine, A.S. & Protic', J. Biol. Chem. 266: 22493-22500, 1991] has been isolated from monkey cell library. The presence of this in complexes bound to DNA was confirmed by immunoblotting. human cognate the UV-DDB gene localized chromosome 11. mRNA expressed all tissues examined, including cells two patients xeroderma pigmentosum (group E) that are...
Abstract The Escherichia coli CMP-N-acetylneuraminic acid (CMP-NeuAc) synthetase gene is located on a 3.3-kilobase (kb) HindIII fragment of the plasmid pSR23 which contains genes for K1 capsule production (Vann, W. F., Silver, R. P., Abeijon, C., Chang, K., Aaronson, W., Sutton, A., Finn, C. Lindner, and Kotsatos, M. (1987) J. Biol. Chem. 262, 17556-17562). CMP-NeuAc expression was increased 10-30-fold by cloning 2.7-kb EcoRI-HindIII onto vector pKK223-3 containing tac promoter. complete...
Accurate detection of somatic mutations is challenging but critical in understanding cancer formation, progression, and treatment. We recently proposed NeuSomatic, the first deep convolutional neural network-based mutation approach, demonstrated performance advantages on silico data.
Previously, using flow cytometric resonance energy transfer and lateral diffusion measurements, we demonstrated that a 95-kDa protein identified by two monoclonal antibodies (OKT27 OKT27b) interacts physically with the 55-kDa alpha of high-affinity interleukin 2 (IL-2) receptor. In present study, this (p95) was purified amino acid sequence data were obtained showed strong homology to human intercellular adhesion molecule 1 (ICAM-1). The identity p95 ICAM-1 confirmed sequential...