A5090567722- TGF-β signaling in diseases
- Developmental Biology and Gene Regulation
- Osteoarthritis Treatment and Mechanisms
- Wnt/β-catenin signaling in development and cancer
- Cancer-related gene regulation
- Connective Tissue Growth Factor Research
- Connective tissue disorders research
- Bone Metabolism and Diseases
- Cell Adhesion Molecules Research
- Animal Genetics and Reproduction
- Protein Kinase Regulation and GTPase Signaling
- Respiratory viral infections research
- Pharmaceutical Economics and Policy
- Neurogenetic and Muscular Disorders Research
- Influenza Virus Research Studies
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Retinal Development and Disorders
- Peptidase Inhibition and Analysis
- Bone Tissue Engineering Materials
- Cytokine Signaling Pathways and Interactions
- Virus-based gene therapy research
- Electrospun Nanofibers in Biomedical Applications
- Cancer-related molecular mechanisms research
United States Food and Drug Administration
2002-2017
Center for Biologics Evaluation and Research
2002-2016
National Institute of Dental and Craniofacial Research
1996-1999
National Institutes of Health
1994-1999
Osaka University
1999
Tohoku Medical and Pharmaceutical University
1999
Partially purified extracts from newborn calf articular cartilage were found to induce and bone when subcutaneously implanted in rats. This activity showed characteristics of morphogenetic proteins (BMPs). Degenerate oligonucleotide primer sets derived the highly conserved carboxyl-terminal region BMP family designed used reverse transcription-polymerase chain reactions with poly(A)+ RNA as template determine which BMPs are produced by chondrocytes. Two novel members transforming growth...
Abstract Objective Ligands and antagonists of the WNT pathway are linked to osteoporosis osteoarthritis. In particular, polymorphisms in FRZB gene, a secreted antagonist, have been associated with The aim this study was examine cartilage bone Frzb −/− mice. Methods gene mice inactivated using Cre/loxP strategy. Three models osteoarthritis were used: collagenase, papain, methylated bovine serum albumin induced. Bone biology studied density measurements microfocal computed tomography....
Cartilage provides the template for endochondral ossification and is crucial determining length width of skeleton. Transgenic mice with targeted expression recombinant cartilage-derived morphogenetic protein-1 (CDMP-1), a member bone protein family, were created to investigate role CDMP-1 in skeletal formation. The exhibited chondrodysplasia expanded cartilage, which consists enlarged hypertrophic zone reduced proliferating chondrocyte zone. Histologically, increased number chondroprogenitor...
Wnt proteins have been implicated in regulating growth and pattern formation a variety of tissues during embryonic development. We previously identified Frzb-1, gene which encodes secreted protein with homology the ligand binding domain to receptor Frizzled, but lacking encoding putative seven transmembrane segments. Frzb-1 has recently shown bind vitro, inhibit activity Xenopus Wnt-8 vivo. report now that mFrzb-1 transcripts display both complementary overlapping expression patterns at...
Abstract Growth/differentiation factor‐5 (GDF5), also known as cartilage‐derived morphogenetic protein‐1 (CDMP‐1), is a secreted signaling molecule that participates in skeletal morphogenesis. Heterozygous mutations GDF5 , which maps to human chromosome 20, occur individuals with autosomal dominant brachydactyly type C (BDC). Here we show BDC locus homogeneous by reporting frameshift mutation segregating the phenotype family whose trait was initially thought map 12. We describe heterozygous...
The bone morphogenetic protein (BMP) family of signaling molecules and their antagonists are involved in patterning the body axis numerous aspects organogenesis. Classical biochemical purification sequencing highly purified fractions containing potent forming activity from bovine cartilage identified several BMPs together with a number other proteins. One such was SMOC-2 (secreted modular calcium-binding protein-2), classified as belonging to BM-40 extracellular Data regarding biological...
The matricellular protein SMOC (Secreted Modular Calcium binding protein) is conserved phylogenetically from vertebrates to arthropods. We showed previously that inhibits bone morphogenetic (BMP) signaling downstream of its receptor via activation mitogen-activated kinase (MAPK) signaling. In contrast, the most prominent effect Drosophila orthologue, pentagone (pent), expanding range BMP during wing patterning. Using deletion constructs we found SMOC-∆EC, lacking extracellular calcium (EC)...
Subtilisin-like Proprotein Convertase 7 (SPC7) is a member of the subtilisin/kexin family pro-protein convertases. It cleaves many pro-proteins to release their active proteins, including members bone morphogenetic protein (BMP) signaling molecules. Other SPCs are known be required during embryonic development but corresponding data regarding SPC7 have not been reported previously.
In an attempt to identify the cell-associated protein(s) through which SMOC (Secreted Modular Calcium binding protein) induces mitogen-activated protein kinase (MAPK) signaling, epidermal growth factor receptor (EGFR) became a candidate. However, although in 32D/EGFR cells, EGFR was phosphorylated presence of commercially available human SMOC-1 (hSMOC-1), only minimal phosphorylation observed Xenopus (XSMOC-1) or SMOC-2. Analysis commercial hSMOC-1 product demonstrated pro-EGF as impurity....
The Wnt family of glycoproteins is involved in numerous developmental and disease processes higher eukaryotes, exerting their action by binding to cell-surface receptors. In the extracellular space, Wnts are negatively regulated secreted antagonists that either bind receptors directly (Dkk1) or molecules themselves (Sfrp-FRZB family), preventing its subsequent receptor. Here we report on a transgenic mouse expressing Cre under control Frzb promoter element. Analysis expression was carried...