A5052680960- Bone Metabolism and Diseases
- Osteoarthritis Treatment and Mechanisms
- RNA Research and Splicing
- TGF-β signaling in diseases
- Congenital limb and hand anomalies
- Cancer-related gene regulation
- Bone health and treatments
- Developmental Biology and Gene Regulation
- Thyroid Disorders and Treatments
- dental development and anomalies
- Wnt/β-catenin signaling in development and cancer
- Hedgehog Signaling Pathway Studies
- Genomics and Chromatin Dynamics
- Cell Adhesion Molecules Research
- NF-κB Signaling Pathways
- Cytokine Signaling Pathways and Interactions
- RNA modifications and cancer
- Connective tissue disorders research
- Cleft Lip and Palate Research
- Neurogenetic and Muscular Disorders Research
- Prenatal Screening and Diagnostics
- Bone and Dental Protein Studies
- Congenital heart defects research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Animal Genetics and Reproduction
KU Leuven
2014-2025
Medical University of Lublin
2014-2025
Laboratory of Molecular Genetics
2022-2025
Erasmus MC
2022
Polish Academy of Sciences
2022
Instytut Biologii Doświadczalnej im. Marcelego Nenckiego
2022
VIB-KU Leuven Center for Cancer Biology
2013
Center for Human Genetics
2013
Charité - Universitätsmedizin Berlin
2008
Vlaams Instituut voor Biotechnologie
1999-2001
Objective. To characterize mesenchymal stem cells (MSCs) from human synovial membrane (SM).Methods.Cell populations were enzymatically released the SM obtained knee joints of adult donors and expanded in monolayer with serial passages at confluence.Cell clones by limiting dilution.At different passages, SM-derived subjected to vitro assays investigate their multilineage potential.Upon treatments, phenotypes cell cultures analyzed histo-and immunohistochemistry semiquantitative reverse...
Activation of transforming growth factor β receptors causes the phosphorylation and nuclear translocation Smad proteins, which then participate in regulation expression target genes. We describe a novel Smad-interacting protein, SIP1, was identified using yeast two-hybrid system. Although SIP1 interacts with MH2 domain receptor-regulated Smads andin vitro, its interaction full-length mammalian cells requires receptor-mediated activation. is new member δEF1/Zfh-1 family two-handed zinc...
Abstract Objective Ligands and antagonists of the WNT pathway are linked to osteoporosis osteoarthritis. In particular, polymorphisms in FRZB gene, a secreted antagonist, have been associated with The aim this study was examine cartilage bone Frzb −/− mice. Methods gene mice inactivated using Cre/loxP strategy. Three models osteoarthritis were used: collagenase, papain, methylated bovine serum albumin induced. Bone biology studied density measurements microfocal computed tomography....
Tumor growth and metastasis are critically dependent on the formation of new blood vessels. The present study found that extracellular matrix protein 1 (ECM1), a newly described secretory glycoprotein, promotes angiogenesis. This was initially suggested by in situ hybridization studies mouse embryos indicating ECM1 message associated with vessels its expression pattern similar to flk-1, recognized marker for endothelium. More direct evidence role angiogenesis provided fact highly purified...
To identify novel marker molecules associated with chondro-osteogenic differentiation, we have set up a differential screening system based on cDNA library subtraction in organ cultures of prenatal mouse mandibular condyles. Differential constructed from vitro cultured condyles allowed the isolation gene, named E25. Full-length E25 is predicted to encode type II integral membrane protein 263 amino acid residues. In situ hybridization experiments show that expressed outer perichondrial rim...
Abstract Objective The balance between destruction and homeostatic or reparative responses determines the outcome of arthritis. Increasing evidence suggests a role for signaling pathways, essential development growth, in maintenance tissue homeostasis attempts at repair. Inappropriate activation such pathways may also have disease progression. We undertook this study to determine effect shifting bone morphogenetic protein (BMP) different mouse models Methods Endogenous levels noggin, BMP...
Abstract The human ubiquitous protein cystinosin is responsible for transporting the disulphide amino acid cystine from lysosomal compartment into cytosol. In humans, Pathogenic mutations of CTNS lead to defective function, intralysosomal accumulation and development cystinosis. Kidneys are initially affected with generalized proximal tubular dysfunction (renal Fanconi syndrome), then disease rapidly affects glomeruli progresses towards end stage renal failure multiple organ dysfunction....
The aim of this study is to histologically and morphologically describe the dental craniofacial manifestations a novel mouse model involving conditional mutation in Smad Interacting Protein 1 (Sip1) gene. Since targeted inactivation Sip1 results early embryonic lethality, tissue-specific was carried out by using Prx1-Cre mice. Embryos at 14.5 days post coitum (dpc), 15.5 dpc, 16.5 dpc 18.5 were analysed, as well newborn five-month-old null mice, means immunohistochemistry (primary antibody:...
Tumor growth and metastasis are critically dependent on the formation of new blood vessels. The present study found that extracellular matrix protein 1 (ECM1), a newly described secretory glycoprotein, promotes angiogenesis. This was initially sug-gested by in situ hybridization studies mouse embryos indicating ECM1 message associated with vessels its expression pattern similar to flk-1, recognized marker for endothelium. More direct evidence role angiogenesis provided fact highly purified...
Noggin is a secreted peptide that binds and inactivates Bone Morphogenetic Proteins, members of the transforming growth factor beta superfamily signaling molecules. In vertebrate limbs, expressed in condensing cartilage immature chondrocytes. Inactivation gene has been reported an inbred 129X1/SvJ mouse genetic background. The null allele was lethal at 18.5 dpc resulted severe hyperplasia together with multiple joint fusions. order to investigate effect background on phenotypic manifestation...
Abstract Progeny of mice treated with the mutagen N-ethyl-N-nitrosourea (ENU) revealed a mouse, designated Longpockets (Lpk), short humeri, abnormal vertebrae, and disorganized growth plates, features consistent spondyloepiphyseal dysplasia congenita (SEDC). The Lpk phenotype was inherited as an autosomal dominant trait. Lpk/+ were viable fertile Lpk/Lpk died perinatally. mapped to chromosome 15 mutational analysis likely candidates from interval Col2a1 missense Ser1386Pro mutation....
Abstract The role of the bone morphogenetic protein (BMP)-signaling mediator Smad1 in osteogenic or chondrogenic differentiation was investigated murine parental mesenchymal progenitors C3H10T½ and its derivatives constitutively expressing BMP-2 (C3H10T½-BMP-2) and, therefore, undergo BMP-mediated osteogenic/chondrogenic development. functions three domains, that is, N-terminal (MH1) domain, C-terminal (MH2) midregional proline-rich linker were documented compared with full-length Smad1. We...
A novel 85-kDa protein secreted by the mouse stromal osteogenic cell line MN7 was identified using two-dimensional polyacrylamide gel electrophoresis (Mathieu, E., Meheus, L., Raymackers, J., and Merregaert, J. (1994) Bone Miner. Res. 9, 903-913). Degenerate primers were used to isolate cDNA coding for this protein. The full-length clone is 1.9 kilobases (kb) codes a of 559 amino acid residues. DNA deduced sequences have no counterparts in public data bases, but structural similarity...