A5048475867- Thyroid Disorders and Treatments
- Growth Hormone and Insulin-like Growth Factors
- Animal Nutrition and Physiology
- Biochemical effects in animals
- Physiological and biochemical adaptations
- Regulation of Appetite and Obesity
- Hypothalamic control of reproductive hormones
- Aquaculture Nutrition and Growth
- Reproductive biology and impacts on aquatic species
- Animal Behavior and Reproduction
- Birth, Development, and Health
- Stress Responses and Cortisol
- Meat and Animal Product Quality
- Animal Genetics and Reproduction
- Plant and animal studies
- Biochemical Analysis and Sensing Techniques
- Animal Vocal Communication and Behavior
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Neuropeptides and Animal Physiology
- Adipose Tissue and Metabolism
- Genetics and Neurodevelopmental Disorders
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Estrogen and related hormone effects
- Aquaculture disease management and microbiota
- Avian ecology and behavior
KU Leuven
2014-2024
Universidad Nacional Autónoma de México
2021
Brigham and Women's Hospital
2017
Virginia Tech
2014
University of Łódź
2013
UCLouvain
2005-2010
Catholic University of America
2009
Zoological Institute
2007
Vlaams Instituut voor Biotechnologie
1993
University of Arizona
1993
In humans, inactivating mutations in the gene of thyroid hormone transporter monocarboxylate 8 (MCT8; SLC16A2) lead to severe forms psychomotor retardation combined with imbalanced serum levels. The MCT8-null mice described here, however, developed without overt deficits but also exhibited distorted 3,5,3′-triiodothyronine (T3) and thyroxine (T4) levels, resulting increased hepatic activity type 1 deiodinase (D1). mutants’ brains, entry T4 was not affected, uptake T3 diminished. Moreover,...
Allan-Herndon-Dudley syndrome (AHDS), a severe form of psychomotor retardation with abnormal thyroid hormone (TH) parameters, is linked to mutations in the TH-specific monocarboxylate transporter MCT8. In mice, deletion Mct8 (Mct8 KO) faithfully replicates AHDS-associated endocrine abnormalities; however, unlike patients, these animals do not exhibit neurological impairments. While transport active TH (T3) across blood-brain barrier strongly diminished KO animals, prohormone (T4) can still...
Monoclonal antibodies (MAbs) against Aspergillus fumigatus galactomannan were produced in rats. Seven of them, EB-A1 through EB-A7, characterized more detail. They all immunoglobulin M antibodies, reacting an indirect enzyme-linked immunosorbent assay with purified A. galactomannan, avidity constants between 2 x 10(9) and 5 10(9)/M. Enzyme-linked inhibition experiments modified synthetic oligomers beta (1----5)galactofuranose demonstrated that the MAbs bound to epitope located on...
In this study, we report the purification, cDNA cloning, and characterization of novel growth hormone-releasing peptide, ghrelin, in chicken (Gallus gallus). Chicken ghrelin is composed 26 amino acids (GSSFLSPTYKNIQQQKDTRKPTARLH) possesses 54% sequence identity with human ghrelin. The serine residue at position 3 (Ser(3)) conserved between mammalian species, as its acylation by either n-octanoic or n-decanoic acid. mRNA predominantly expressed stomach, where it present proventriculus but...
Organic anion-transporting polypeptide 1c1 (Oatp1c1) (also known as Slco1c1 and Oatp14) belongs to the family of Oatp has been shown facilitate transport T(4). In rodent brain, Oatp1c1 is highly enriched in capillary endothelial cells choroid plexus structures where it may mediate entry T(4) into central nervous system. Here, we describe generation first analysis Oatp1c1-deficient mice. knockout (KO) mice were born with expected frequency, not growth retarded, developed without any overt...
Pronounced alterations in serum thyroid hormone levels occur during critical illness. T3 decreases and rT3 increases, the magnitudes of which are related to severity disease. It is unclear whether these changes associated with decreased tissue concentrations and, thus, reduced bioactivity. PATIENTS AND STUDY QUESTIONS: We therefore investigated, 79 patients who died after intensive care did or not receive treatment, total correspond liver muscle. Furthermore, we investigated relationship...
Despite the known importance of thyroid hormones (THs) in vertebrate growth and development, role tissue-specific TH activation early embryogenesis remains unclear. We therefore examined function type 2 iodothyronine deiodinase (D2), one two enzymes catalyzing conversion T4 to T3, developing zebrafish embryos (Danio rerio). Microinjection with antisense oligonucleotides targeting either D2 translation start site or splice junction between first exon intron induced delays development...
Prolonged critically ill patients have low circulating thyroid hormone (TH) levels without a rise in TSH, condition labeled 'the tri-iodothyronine (T(3)) syndrome'. Currently, it is not clear whether this represents an adaptive response. We examined the role of TH transporters monocarboxylate transporter 8 (MCT8, also known as SLC16A2) and MCT10 pathogenesis T(3) syndrome prolonged critical illness.A clinical observational study intervention vivo animal model illness. Gene expression MCT8...
Seasonal animals adapt their physiology and behaviour in anticipation of climate change to optimise survival offspring. Intra-hypothalamic thyroid hormone signalling plays an important role seasonal responses mammals birds. In the F344 rat, photoperiod stimulates profound changes food intake, body weight reproductive status. Previous investigations rat have suggested a for metabolism, but only considered Dio2 expression, which was elevated long day photoperiods. Microarray analysis used...
In patients, inactivating mutations in the gene encoding thyroid hormone-transporting monocarboxylate transporter 8 (Mct8) are associated with severe mental and neurological deficits disturbed hormone levels. The latter phenotype characterized by high T3 low T4 serum concentrations is replicated Mct8 knockout (ko) mice, indicating that MCT8 deficiency interferes production and/or metabolism. Our studies of ko mice indeed revealed increased thyroidal without overt signs a hyperactive gland....
Thyroid hormone (TH) is essential for vertebrate brain development. Most research on TH and neuronal development focuses late development, mainly the perinatal period in mammals. However, human infants neuromotor correlates best with maternal levels first trimester of pregnancy, suggesting that signaling could affect early Studying embryogenesis mammals experimentally challenging. In contrast, free-living embryos, such as Xenopus laevis, permit physiological experimentation independent...
Exposure to appropriate levels of thyroid hormones (THs) at the right time is key importance for normal development in all vertebrates. Type 3 iodothyronine deiodinase (D3) prime TH-inactivating enzyme, and its expression highest early stages vertebrate development, implying that it may be necessary shield developing tissues from overexposure THs. We used antisense morpholino knockdown examine role D3 during zebrafish. Zebrafish possess 2 genes, dio3a dio3b. Here, we show both genes are...
The monocarboxylate transporter Mct10 (Slc16a10; T-type amino acid transporter) facilitates the cellular transport of thyroid hormone (TH) and shows an overlapping expression with well-established TH Mct8. Because Mct8 deficiency is associated distinct tissue-specific alterations in metabolism, we speculated that inactivation may compromise homeostasis as well. However, analysis knockout (ko) mice revealed normal serum levels tissue content contrast to ko are characterized by high T3, low...
Methimazole (MMI) is an anti-thyroid drug used in the treatment of chronic hyperthyroidism. There is, however, some debate about its use during pregnancy as MMI known to cross mammalian placenta and reach developing foetus. A similar problem occurs birds, where deposited egg taken up by embryo. To investigate whether maternally derived can have detrimental effects on embryonic development, we treated laying hens with (0.03% drinking water) measured total reduced contents tissues embryos at...
DNA damage contributes to the process of aging, as underscored by premature aging syndromes caused defective repair. Thyroid state changes during but underlying mechanisms remain elusive. Since thyroid hormone (TH) is a key regulator metabolism, in TH signaling have widespread effects. Here, we reveal significant common transcriptomic signature livers from hypothyroid mice, repair-deficient mice with severe (Csbm/m/Xpa-/-) or intermediate (Ercc1-/Δ-7) progeria and naturally aged mice. A...
Thyroid hormone (TH) balance is essential for vertebrate development. Deiodinase type 1 (D1) and 2 (D2) increase deiodinase 3 (D3) decreases local intracellular levels of T3, the most important active TH. The role deiodinase-mediated TH effects in early development only partially understood. Therefore, we investigated deiodinases during zebrafish until 96 hours post fertilization at level transcriptome (microarray), biochemistry, morphology physiology using morpholino (MO) knockdown....