A5041583136- Single-cell and spatial transcriptomics
- RNA modifications and cancer
- Renal Diseases and Glomerulopathies
- Viral Infections and Immunology Research
- T-cell and B-cell Immunology
- Extracellular vesicles in disease
- Cancer Genomics and Diagnostics
- Systemic Lupus Erythematosus Research
- Immunotherapy and Immune Responses
- Phagocytosis and Immune Regulation
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Immune cells in cancer
- Viral Infectious Diseases and Gene Expression in Insects
- Pancreatic and Hepatic Oncology Research
- interferon and immune responses
- RNA regulation and disease
- Insect Resistance and Genetics
- Plant Virus Research Studies
- CRISPR and Genetic Engineering
- Molecular Biology Techniques and Applications
- Cancer Cells and Metastasis
- Mitochondrial Function and Pathology
- Immune Cell Function and Interaction
- Viral gastroenteritis research and epidemiology
Center for Cancer Research
2020-2023
National Institutes of Health
2023
Institute of Medical Sciences
2023
National Cancer Institute
2019-2023
Leidos (United States)
2020-2021
Frederick National Laboratory for Cancer Research
2019-2021
Leidos Biomedical Research Inc. (United States)
2020-2021
SUNY Downstate Health Sciences University
2013-2016
State University of New York
2016
Solid tumors elicit a detectable immune response including the infiltration of tumor-associated macrophages (TAMs). Unfortunately, this is co-opted into contributing toward tumor growth instead preventing its progression. We seek to reestablish an antitumor by selectively targeting surface receptors and endogenous signaling processes macrophage subtypes driving cancer RP-182 synthetic 10-mer amphipathic analog host defense peptides that induces conformational switch mannose receptor CD206...
Abstract Ribosomal recruitment of cellular mRNAs depends on binding eIF4F to the mRNA’s 5′-terminal ‘cap’. The minimal ‘cap0’ consists N7-methylguanosine linked first nucleotide via a 5′-5′ triphosphate (ppp) bridge. Cap0 is further modified by 2′-O-methylation next two riboses, yielding ‘cap1’ (m7GpppNmN) and ‘cap2’ (m7GpppNmNm). However, some viral RNAs lack 2′-O-methylation, whereas others contain only ppp- at their 5′-end. Interferon-induced proteins with tetratricopeptide repeats...
Ribosomal attachment to mammalian capped mRNAs is achieved through the cap–eukaryotic initiation factor 4E (eIF4E)–eIF4G–eIF3–40S chain of interactions, but mechanism by which mRNA enters mRNA-binding channel 40S subunit remains unknown. To investigate this process, we recapitulated on in vitro using a reconstituted translation system. Formation complexes at 5′-terminal AUGs was stimulated eIF4E–cap interaction and followed “the first AUG” rule, indicating that it did not occur backward...
IntroductionIndividuals with focal segmental glomerular sclerosis (FSGS) typically undergo kidney biopsy only once, which limits the ability to characterize cell gene expression over time.MethodsWe used single-cell RNA sequencing (scRNA-seq) explore disease-related molecular signatures in urine cells from subjects FSGS. We collected 17 samples 12 FSGS and captured these as 23 samples. The inflammatory renal epithelial immune were evaluated bulk data sets of minimal change disease (MCD) (The...
Trichoplusiani derived cell lines are commonly used to enable recombinant protein expression via baculovirus infection generate materials approved for clinical use and in trials. In order develop systems biology genome engineering tools improve this host, we performed de novo assembly of the Trichoplusiani-derived line Tni-FNL.By integration PacBio single-molecule sequencing, Bionano optical mapping, 10X Genomics linked-reads data, have produced a draft Tni-FNL.Our contains 280 scaffolds,...
Abstract The desmoplastic stroma of pancreatic cancers forms a physical barrier that impedes intratumoral drug delivery. Attempts to modulate the increase delivery administered chemotherapy have not shown positive clinical results thus far, and preclinical reports in which chemotherapeutic drugs were coadministered with antistromal therapies did universally demonstrate increased genotoxicity despite levels. In this study, we tested whether TGFβ antagonism can break stromal barrier, enhance...
How CD4+ lineage gene expression is initiated in differentiating thymocytes remains poorly understood. Here, we show that the paralog transcription factors Zfp281 and Zfp148 control both this process cytokine by T helper cell type 2 (TH2) effector cells. Genetic, single-cell, spatial transcriptomic analyses showed these promote intrathymic differentiation of class II major histocompatibility complex (MHC II)-restricted thymocytes, including lineage-committing factor Thpok. In peripheral...
Abstract Single-cell RNA sequencing (scRNA-seq) has become a very powerful technology for biomedical research and is becoming much more affordable as methods continue to evolve, but it unknown how reproducible different platforms are using bioinformatics pipelines, particularly the recently developed scRNA-seq batch correction algorithms. We carried out comprehensive multi-center cross-platform comparison on standard reference samples. compared six pre-processing seven normalization...
Abstract The diagnosis of focal segmental glomerulosclerosis (FSGS) requires a renal biopsy, which is invasive and can be problematic in children some adults. We used single cell RNA-sequencing to explore disease-related cellular signatures 23 urine samples from 12 FSGS subjects. identified immune cells, predominantly monocytes, epithelial including podocytes. Analysis revealed M1 M2 monocyte subsets, podocytes showing high expression genes for epithelial-to-mesenchymal transition (EMT)....
Abstract T cells are critical components of the adaptive immune system; they develop from bone-marrow derived precursors that migrate to thymus. There, undergo a sequence lineage specification and commitment steps give rise multiple cell lineages serving essential functions in defenses against infection homeostasis. The diversity thymocytes, their complex interactions with thymic stroma, including epithelial other hematopoietic compartments, have hampered our understanding development. For...