A5080397665- Advanced NMR Techniques and Applications
- Plant biochemistry and biosynthesis
- Biochemical and Molecular Research
- Enzyme Structure and Function
- X-ray Diffraction in Crystallography
- NMR spectroscopy and applications
- Crystallization and Solubility Studies
- Protein Structure and Dynamics
- Molecular spectroscopy and chirality
- Microbial Natural Products and Biosynthesis
- Solid-state spectroscopy and crystallography
- Bone health and treatments
- ATP Synthase and ATPases Research
- Lipid Membrane Structure and Behavior
- Hemoglobin structure and function
- Computational Drug Discovery Methods
- Metal-Catalyzed Oxygenation Mechanisms
- Trypanosoma species research and implications
- HIV/AIDS drug development and treatment
- Metalloenzymes and iron-sulfur proteins
- Research on Leishmaniasis Studies
- Electrochemical Analysis and Applications
- Click Chemistry and Applications
- Organophosphorus compounds synthesis
- Spectroscopy and Quantum Chemical Studies
University of Illinois Urbana-Champaign
2016-2025
Instituto de Estudios Avanzados
2006-2016
Urbana University
1993-2015
Tianjin Institute of Industrial Biotechnology
2012-2015
Chinese Academy of Sciences
2012-2015
Institut Lavoisier de Versailles
2010-2015
Johns Hopkins University
2015
Institute of Molecular Biology and Biophysics
2012
Centre National de la Recherche Scientifique
2005-2010
Iowa State University
2005-2010
Staphylococcus aureus produces hospital- and community-acquired infections, with methicillin-resistant S. posing a serious public health threat. The golden carotenoid pigment of aureus, staphyloxanthin, promotes resistance to reactive oxygen species host neutrophil-based killing, early enzymatic steps in staphyloxanthin production resemble those for cholesterol biosynthesis. We determined the crystal structures dehydrosqualene synthase (CrtM) at 1.58 angstrom resolution, finding structural...
Recent theoretical developments permit the prediction of 1H, 13C, 15N, and 19F nuclear magnetic resonance chemical shifts in proteins offer new ways analyzing secondary tertiary structure as well for probing protein electrostatics. For phi, psi torsion angles dominate shielding C alpha beta, but addition hydrogen bonding electrostatics gives even better accord with experiment. 15NH, side chain (chi 1) are also important, nearest neighbor sequence effects, whereas 1HN, is particularly...
Inorganic polyphosphate (polyP) has been identified and measured in human platelets. Millimolar levels (in terms of Pi residues) short chain polyP were found. The presence approximately 70-75 phosphate units was by 31P NMR urea-polyacrylamide gel electrophoresis platelet extracts. An analysis dense granules, purified using metrizamide gradient centrifugation, indicated that preferentially located these organelles. This confirmed visualization the granules 4',6-diamidino-2-phenylindole its...
The trans hydrogen bond 3hJNC' coupling observed between peptide groups in proteins is shown to be mediated by a closed shell, noncovalent interaction the donor atom and acceptor oxygen atom. magnitude of an exponential function mutual penetration nonbonding van der Waals shells isolated fragments. Our results also show that JFF, through-space two nonbonded fluorine nuclei organic molecules protein, exhibits similar dependence upon monomer charge densities. These support idea existence...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSpectroscopic studies of specifically deuterium labeled membrane systems. Nuclear magnetic resonance investigation the effects cholesterol in model systemsEric Oldfield, Michael Meadows, David Rice, and Russell JacobsCite this: Biochemistry 1978, 17, 14, 2727–2740Publication Date (Print):July 11, 1978Publication History Published online1 May 2002Published inissue 11 July...
We show that copper-containing metal-organic nanoparticles (MONPs) are readily synthesized via Cu(II)-mediated intramolecular cross-linking of aspartate-containing polyolefins in water. In situ reduction with sodium ascorbate yields Cu(I)-containing MONPs serve as highly efficient supramolecular catalysts for alkyne-azide "click chemistry" reactions, yielding the desired 1,4-adducts at low parts per million catalyst levels. The have toxicity and metal loadings, making them convenient, green...
We report the discovery of a series new drug leads that have potent activity against Mycobacterium tuberculosis as well other bacteria, fungi, and malaria parasite. The compounds are analogues (TB) SQ109 (1), which has been reported to act by inhibiting transporter called MmpL3, involved in cell wall biosynthesis. show 1 also target enzymes menaquinone biosynthesis electron transport, respiration ATP biosynthesis, uncouplers, collapsing pH gradient membrane potential used power transporters....
In both cancer and infections, diseased cells are presented to human Vγ9Vδ2 T through an 'inside out' signalling process whereby structurally diverse phosphoantigen (pAg) molecules sensed by the intracellular domain of butyrophilin BTN3A11-4. Here we show how-in humans alpaca-multiple pAgs function as 'molecular glues' promote heteromeric association between domains BTN3A1 similar BTN2A1. X-ray crystallography studies visualized that engagement with forms a composite interface for direct...
We report the first combined application of solid-state electrochemical NMR (EC NMR), cyclic voltammetry (CV), and potentiostatic current generation to investigate topic ruthenium promotion MeOH electro-oxidation over nanoscale platinum catalysts. The CV EC results give evidence for two types CO: CO on essentially pure Pt Pt/Ru islands. There is no rapid exchange between populations. molecules primarily domains behave much like Pt, with there being little effect Ru Knight shift or Korringa...
We have investigated the effects in vitro of a series bisphosphonates on proliferation Trypanosoma cruzi, brucei rhodesiense, Leishmania donovani, Toxoplasma gondii, and Plasmodium falciparum. The results show that nitrogen-containing type used bone resorption therapy significant activity against parasites, with aromatic species having some cases nanomolar or low-micromolar IC50 values parasite replication (e.g. o-risedronate, = 220 nM for T. rhodesiense; risedronate, 490 gondii). In...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTHigh-resolution oxygen-17 NMR of solidsSuzanne Schramm and Eric OldfieldCite this: J. Am. Chem. Soc. 1984, 106, 9, 2502–2506Publication Date (Print):May 1, 1984Publication History Published online1 May 2002Published inissue 1 1984https://doi.org/10.1021/ja00321a002RIGHTS & PERMISSIONSArticle Views374Altmetric-Citations213LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum full text article downloads since November 2008 (both PDF...
Journal Article Specific Genetic Change in Tumors Associated With von Hippel-Lindau Disease Get access Kalman Tory, Tory Laboratory of Immunobiology, Naitonal Cancer Institute-Frederick Research FacilityFrederick, MD Search for other works by this author on: Oxford Academic PubMed Google Scholar Hiltrud Brauch, Brauch Marston Linehan, Linehan Surgery Branch, Division Treatment, National Institute, Institues HealthBethesda, David Barba, Barba Surgical Neurology Branch Edward Oldfield,...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPrediction of silicon-29 nuclear magnetic resonance chemical shifts using a group electronegativity approach: applications to silicate and aluminosilicate structuresNathan Janes Eric OldfieldCite this: J. Am. Chem. Soc. 1985, 107, 24, 6769–6775Publication Date (Print):November 1, 1985Publication History Published online1 May 2002Published inissue 1 November 1985https://doi.org/10.1021/ja00310a004Request reuse permissionsArticle...
We have obtained high-resolution solid-state NMR spectra of a variety nonintegral-spin quadrupolar nuclei (23Na, 51V, and 55Mn) under conditions ‘‘magic-angle’’ sample-spinning (MASS) ‘‘variable-angle’’ (VASS). show for systems in which the second-order quadrupole interaction dominates breadth (1/2,−1/2) spin transition, that optimum line narrowing is achieved by rapid sample rotation at angles other than familiar θ=54.7° ‘‘magic-angle.’’ The effect originates more complex angle dependence...
Oxygen reduction reaction (ORR) measurements and 195Pt electrochemical nuclear magnetic resonance (EC-NMR) spectroscopy were combined to study a series of carbon-supported platinum nanoparticle electrocatalysts (Pt/CB) with average diameters in the range roughly 1–5 nm. ORR rate constants H2O2 yields evaluated from hydrodynamic voltammograms did not show any particle size dependency. The apparent activation energy 37 kJ mol−1, obtained for constant, was identical that bulk electrodes. Pt/CB...
Considerable effort has focused on the development of selective protein farnesyl transferase (FTase) and geranylgeranyl (GGTase) inhibitors as cancer chemotherapeutics. Here, we report a new strategy for anticancer therapeutic agents involving inhibition diphosphate synthase (FPPS) (GGPPS), two enzymes upstream FTase GGTase, by lipophilic bisphosphonates. Due to dual site targeting decreased polarity, compounds have activities far greater than do current bisphosphonate drugs in inhibiting...