A5020950344- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Protease and Inhibitor Mechanisms
- Cell Adhesion Molecules Research
- Synthesis and biological activity
- Synthesis and Biological Evaluation
- Oil Spill Detection and Mitigation
- Glycosylation and Glycoproteins Research
- Photosynthetic Processes and Mechanisms
- Carcinogens and Genotoxicity Assessment
- DNA Repair Mechanisms
- Coastal and Marine Management
- Synthesis and Reactivity of Sulfur-Containing Compounds
- Tuberculosis Research and Epidemiology
- Studies on Chitinases and Chitosanases
- Plant biochemistry and biosynthesis
- Helminth infection and control
- Biochemical and Molecular Research
- Ocean Acidification Effects and Responses
- Computational Drug Discovery Methods
- RNA Interference and Gene Delivery
- Immunotherapy and Immune Responses
- Cellular transport and secretion
- Toxin Mechanisms and Immunotoxins
- Metal complexes synthesis and properties
Instituto Venezolano de Investigaciones Científicas
1984-2018
Instituto de Estudios Avanzados
2006
Institut Cochin
2006
Central University of Venezuela
2006
Inserm
2006
Universidad Autónoma de Madrid
2004
University of Illinois Urbana-Champaign
2001
We have investigated the effects in vitro of a series bisphosphonates on proliferation Trypanosoma cruzi, brucei rhodesiense, Leishmania donovani, Toxoplasma gondii, and Plasmodium falciparum. The results show that nitrogen-containing type used bone resorption therapy significant activity against parasites, with aromatic species having some cases nanomolar or low-micromolar IC50 values parasite replication (e.g. o-risedronate, = 220 nM for T. rhodesiense; risedronate, 490 gondii). In...
There is no effective treatment for the prevalent chronic form of Chagas' disease in Latin America. Its causative agent, protozoan parasite Trypanosoma cruzi, has an essential requirement ergosterol, and ergosterol biosynthesis inhibitors, such as antifungal drug posaconazole, have potent trypanocidal activity. The antiarrhythmic compound amiodarone, frequently prescribed symptomatic patients, also recently been shown to We now show here first time that amiodarone direct activity against T....
The complexes [Cu(CTZ)(4)]Cl(2).2H(2)O (1), [Cu(CTZ)Cl(2)](2) (2), [Cu(KTZ)(3)Cl(2)] (3), and [Cu(KTZ)Cl(2)](2).2H(2)O (4) were prepared by reaction of CuCl(2) with CTZ KTZ (where = 1-[[(2-chlorophenyl)diphenyl]methyl]-1H-imidazole cis-1-acetyl-4-[4-[[2-(2,4-dichlorophenyl)-2-(1H-imidazol-1-ylmethyl)-1,3-dioxolan-4-yl]methoxy]phenyl]piperazine), respectively, in acetonitrile at different ligand to metal molar ratios. Gold [Au(PPh(3))(CTZ)]PF(6) (5) [Au(PPh(3))(KTZ)]PF(6).H(2)O (6)...
We investigated the mechanism of action metabolically stable lysophospholipid analogues (LPAs), with potent anti-tumour and anti-protozoal activity against Trypanosoma cruzi, causative agent Chagas' disease. Against axenically grown epimastigote form parasite, IC50s after 120 h for ET-18-OCH3, miltefosine ilmofosine were 3, 1 3 μM, respectively; at higher concentrations immediate lytic effects observed. Eradication intracellular amastigote, inside Vero cells, was achieved 0.1, 0.1 μM...
Investigation of the antiproliferative synergy lysophospholipid analogues (LPAs) edelfosine, ilmofosine and miltefosine with ergosterol biosynthesis inhibitor ketoconazole against Trypanosoma cruzi.The effect LPAs, their combination was evaluated epimastigotes intracellular amastigotes by parameter IC50 leading to construction isobolograms, for determination a synergic effect. For epimastigotes, ultrastructural damage induced these treatments transmission scanning electron microscopy.Synergy...
ABSTRACT We describe the in vitro antiproliferative effects of new triazole derivative UR-9825 against protozoan parasite Trypanosoma ( Schizotrypanum ) cruzi , causative agent Chagas' disease Latin America. The compound was found to be extremely active cultured (epimastigote) form parasite, equivalent that present reduviid vector, with a MIC 30 nM, concentration 33-fold lower than required reference ketoconazole. At MIC, growth arrest coincided depletion parasite's 4,14-desmethyl endogenous...
ABSTRACT. Respiration, oxidative phosphorylation, and the corresponding changes in membrane potential (ΔΨ) of Trypanosoma cruzi epimastigotes grown either liver infusion‐tryptose (LIT) or brain heart infusion (BHI) culture medium were assayed situ using digitonin to render their plasma permeable succinate, ADP, safranine O, other small molecules. When cells permeabilized with 64 μM digitonin, a concentration previously used epimastigotes, ability LIT sustain phosphorylation was demonstrated...
The temporal and functional relationships between DNA events of meiotic recombination synaptonemal complex formation are a matter discussion within the field. To analyse this subject in grasshoppers, organisms that have been considered as models for studies many years, we studied localization phosphorylated histone H2AX (γ‐H2AX), which marks sites double‐strand breaks (DSBs), combination with cohesin SMC3 recombinase Rad51. We show loss γ‐H2AX staining is spatially temporally linked to...
The ocean is intrinsically linked to human health as it provides food and wellbeing, yet shifts in its dynamics can pose climate-ecological risks, such harmful algal blooms (HABs) that impact the economy of coastal communities. For decades, Ecuadorian communities have witnessed seasonal blooms, events are driven by factors including complex ocean-climate interactions, nutrient availability, ecological variables. However, little known about risk perceived populations regarding events....
Abstract The possible differential response of adherent and nonadherent cells the same tumor type to pyrimidine analogues has been investigated. We show that bromodeoxyuridine (BUdR) increases interactions attached with their substrate without markedly affecting cell adhesion properties when these are not anchored. However, evidence for an adhesion‐independent both types BUdR obtained lectin binding assays using 125 I‐labelled Lens culinaris agglutinin (LCA). This revealed a greatly...
Abstract Growth of highly invasive B16 melanoma BL6 cells with bromodeoxyuridine (BUdR) decreases in vitro cell detachment and modulates extrapulmonary growth vivo. We now show: (1) The presence an 80 kd glycoprotein the Triton‐insoluble matrix control but not corresponding fractions from BUdR‐treated poorly metastatic Flr cells. (2) two last mentioned reveal glycoproteins about 55‐58 kd. (3) Mild trypsin treatment intact before preparation leads to preferential disappearance component...