A5062853660- Extracellular vesicles in disease
- Herpesvirus Infections and Treatments
- Reproductive System and Pregnancy
- Cytomegalovirus and herpesvirus research
- MicroRNA in disease regulation
- Parvovirus B19 Infection Studies
- COVID-19 Impact on Reproduction
- Pregnancy and preeclampsia studies
- interferon and immune responses
- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Gestational Diabetes Research and Management
- Virus-based gene therapy research
- Chromosomal and Genetic Variations
- Evolution and Genetic Dynamics
- Viral Infections and Immunology Research
- Circular RNAs in diseases
- Prenatal Screening and Diagnostics
- RNA modifications and cancer
- Plant Virus Research Studies
- RNA regulation and disease
- Poxvirus research and outbreaks
- Energy and Environment Impacts
- HIV Research and Treatment
Fred Hutch Cancer Center
2017-2023
Cape Town HVTN Immunology Laboratory / Hutchinson Centre Research Institute of South Africa
2022
Cancer Research Center
2022
Magee-Womens Research Institute
2013-2017
University of Pittsburgh
2012-2017
Placental trophoblasts form the interface between fetal and maternal environments serve to limit maternal–fetal spread of viruses. Here we show that cultured primary human placental are highly resistant infection by a number viruses and, importantly, confer this resistance nonplacental recipient cells exosome-mediated delivery specific microRNAs (miRNAs). We miRNA members chromosome 19 cluster, which almost exclusively expressed in placenta, packaged within trophoblast-derived exosomes...
Mechanisms to protect against viral infections are crucial during pregnancy as maternal-fetal transmission can have serious pathological outcomes, including fetal infection and its sequelae, such growth restriction, birth defects, and/or death. The trophoblast forms the interface between feto-placental unit maternal blood, is therefore a critical physical immunological barrier restrict spread of pathogens into microenvironment. Recently, we found that primary human placental (PHT) cells...
Recently, we compared amino acid sequences of the E2 glycoprotein natural North American eastern equine encephalitis virus (NA-EEEV) isolates and demonstrated that naturally circulating viruses interact with heparan sulfate (HS) this interaction contributes to extreme neurovirulence EEEV (C. L. Gardner, G. D. Ebel, K. Ryman, W. B. Klimstra, Proc. Natl. Acad. Sci. U. S. A., 108:16026-16031, 2011). In current study, have examined contribution HS binding each three lysine residues in 71-to-77...
ICP4 is the major activator of herpes simplex virus (HSV) transcription. Previous studies have defined several regions that are important for viral gene expression, including a DNA binding domain and transactivation domains contained in C-terminal N-terminal 520 274 amino acids, respectively. Here we show 210 acids required interactions with components TFIID mediator and, as consequence, necessary activation genes. A mutant deleted 30 to 210, d3-10, was unable complement an null at level...
While cytomegalovirus (CMV) infections are often limited in host range by lengthy coevolution with a single species, few CMVs known to deviate from this rule. For example, rhesus macaque CMV (RhCMV), model for human (HCMV) pathogenesis and vaccine development, can replicate cells, as well cells. Both HCMV RhCMV encode species-specific antagonists of the broadly acting cell restriction factor protein kinase R (PKR). Although antagonist PKR, rTRS1, has very activity against here, we show it is...
ABSTRACT Myxovirus resistance proteins A and B (MxA MxB) are interferon-induced that exert antiviral activity against a diverse range of RNA DNA viruses. In primates, MxA has been shown to inhibit myxoviruses, bunyaviruses, the hepatitis virus, whereas MxB restricts retroviruses herpesviruses. As result their conflicts with viruses, both genes have undergoing diversifying selection during primate evolution. Here, we investigate how evolution in primates affected its restriction contrast...
Myxovirus resistance proteins (MxA and MxB) are interferon-induced that exert antiviral activity against a diverse range of RNA DNA viruses. In primates, MxA has been shown to inhibit myxoviruses, bunyaviruses, hepatitis B virus, whereas MxB restricts retroviruses herpesviruses. As result their conflicts with viruses, both genes have undergoing diversifying selection during primate evolution. Here, we investigate how evolution in primates affected its restriction contrast human MxB, find...