A5034203294- Listeria monocytogenes in Food Safety
- Cell Adhesion Molecules Research
- Lipid Membrane Structure and Behavior
- Essential Oils and Antimicrobial Activity
- Autophagy in Disease and Therapy
- Microbial Inactivation Methods
- Antimicrobial Peptides and Activities
- Vibrio bacteria research studies
- Immune Response and Inflammation
- Cellular Mechanics and Interactions
- Immunotherapy and Immune Responses
- Inflammasome and immune disorders
- Viral Infectious Diseases and Gene Expression in Insects
- Metalloenzymes and iron-sulfur proteins
- Salmonella and Campylobacter epidemiology
- Biochemical and Structural Characterization
- RNA Interference and Gene Delivery
- Reproductive System and Pregnancy
- Research on Leishmaniasis Studies
- T-cell and B-cell Immunology
- Eosinophilic Disorders and Syndromes
- Preterm Birth and Chorioamnionitis
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Bacterial biofilms and quorum sensing
- Microbial Fuel Cells and Bioremediation
The Ohio State University
2014-2024
The Ohio State University Wexner Medical Center
2017-2024
Interface (United States)
2009-2017
Columbus Oncology and Hematology Associates
2014
Czech Academy of Sciences, Institute of Microbiology
2012
Ohio Supercomputer Center
2008
Inserm
1997-2007
University of Michigan–Ann Arbor
2006-2007
Institut Pasteur
2004-2005
University of Bern
2000-2003
Upon infection, pathogens reprogram host gene expression. In eukaryotic cells, genetic reprogramming is induced by the concerted activation/repression of transcription factors and various histone modifications that control DNA accessibility in chromatin. We report here bacterial pathogen Listeria monocytogenes induces a dramatic dephosphorylation H3 as well deacetylation H4 during early phases infection. This effect mediated major listerial toxin listeriolysin O pore-forming-independent...
Intracellular pathogens have evolved diverse strategies to invade and survive within host cells. Among the most studied facultative intracellular pathogens, Listeria monocytogenes is known express two invasins-InlA InlB-that induce bacterial internalization into nonphagocytic The pore-forming toxin listeriolysin O (LLO) facilitates escape from vesicle cytoplasm, where bacteria divide undergo cell-to-cell spreading via actin-based motility. In present study we demonstrate that in addition...
Significance We report the discovery of fundamental roles for noncanonical inflammasome molecule Caspase-4/11 in promoting pathological inflammatory and prothrombotic pathways severe acute respiratory syndrome coronavirus 2 (SARS–CoV-2) infections. Our work demonstrates that Caspase-11 has a broader role immune responses beyond its previously appreciated effects bacterial Further, we show Caspase-11–deficient mice infected with SARS–CoV-2 fare significantly better terms overall illness, lung...
Listeria monocytogenes uptake by nonphagocytic cells is promoted the bacterial invasion proteins internalin and InlB, which bind to their host receptors E-cadherin hepatocyte growth factor receptor (HGF-R)/Met, respectively. Here, we present evidence that plasma membrane organization in lipid domains critical for uptake. Cholesterol depletion methyl-β-cyclodextrin reversibly inhibited entry. Lipid raft markers, such as glycosylphosphatidylinositol-linked proteins, a myristoylated...
In response to chemoattractants neutrophils extend an actin-rich pseudopod, which imparts morphological polarity and is required for migration. Even when stimulated by isotropic bath of chemoattractant, exhibit persistent polarization continued lamellipod formation at the front, suggesting that cells establish internal polarity. this report, we show perturbing lipid organization depleting plasma membrane cholesterol levels reversibly inhibits cell Among other receptor-mediated responses, β2...
On treatment with chemoattractant, the neutrophil plasma membrane becomes organized into detergent-resistant domains (DRMs), distribution of which is intimately correlated cell polarization. Plasma at front polarized cells susceptible to extraction by cold Triton X-100, whereas rear resistant extraction. After X-100 extraction, DRM components, including transmembrane proteins CD44 and CD43, GPI-linked CD16, lipid analog, DiIC(16), are retained within uropods bodies. Furthermore, CD43...
ABSTRACT CASP4/caspase-11-dependent inflammasome activation is important for the clearance of various Gram-negative bacteria entering host cytosol. Additionally, CASP4 modulates actin cytoskeleton to promote maturation phagosomes harboring intracellular pathogens such as Legionella pneumophila but not those enclosing nonpathogenic bacteria. Nevertheless, this non-inflammatory role regarding trafficking vacuolar remains poorly understood. Macroautophagy/autophagy, a catabolic process within...
Listeria monocytogenes is a facultative intracellular pathogen that infects wide variety of cells, causing the life-threatening disease listeriosis. L. virulence factors include two surface invasins, InlA and InlB, known to promote bacterial uptake by host secreted pore-forming toxin listeriolysin O (LLO), which disrupts phagosome allow proliferation in cytosol.
Listeria monocytogenes is a facultative intracellular pathogen that infects large diversity of host cells, including macrophages. To avoid the phagosome microbicidal environment, L. secretes pore-forming toxin (listeriolysin O, LLO) releases bacterium into cytoplasm. We hypothesized α-defensins (HNPs) and/or humanized θ-defensin (RC-1) peptides produced by human and non-human primate neutrophils, respectively, cooperate with macrophages to control infection. Our results establish HNP-1 RC-1...
Obligate intracellular pathogens such as Leishmania specifically target host phagocytes for survival and replication. Phosphoinositide 3-kinase γ (PI3Kγ), a member of the class I PI3Ks that is highly expressed by leukocytes, controls cell migration initiating actin polymerization cytoskeletal reorganization, which are processes also critical phagocytosis. In this study, we demonstrate IB PI3K, PI3Kγ, plays role in pathogenesis chronic cutaneous leishmaniasis caused L. mexicana . Using...
The placenta controls the growth of fetus and ensures its immune protection. Key to these functions, syncytiotrophoblast (SYN) is a syncytium formed by fusion underlying mononuclear trophoblasts. SYN covers placental surface bathed in maternal blood mediate nutritional waste exchanges between mother fetus. bacterial pathogen Listeria monocytogenes breaches trophoblast barrier infects placental/fetal unit resulting poor pregnancy outcomes. In this work, we analyzed L. intracellular lifecycle...
The signalling pathway for the hepatocyte growth factor receptor, Met/HGF-R, is hijacked by bacterial surface protein InlB to induce Listeria monocytogenes entry into non-phagocytic cells. We previously showed that invades host cells interacting with specialized microdomains of plasma membrane called lipid rafts. In this study, we analysed in living events are crucial using a fluorescence resonance energy transfer-based microscopic method. Phosphoinositide (PI) 3-kinase activity and Rac1...
Listeria monocytogenes is an intracellular, foodborne gastrointestinal pathogen that primarily responsible for causing listeriosis or food poisoning in otherwise healthy individuals. Infections arise during pregnancy within immune compromised individuals are much more serious resulting the risk of fetal termination fatality postpartum former and septicemia meningitis with a 20% rate latter. While roles internalin proteins listeriolysin-O infection process well characterized, specific...
ABSTRACT Listeria monocytogenes is responsible for the life-threatening food-borne disease listeriosis. This mainly affects elderly and immunocompromised individuals, causing bacteremia meningoencephalitis. In pregnant women, L. infection leads to abortion severe of fetus or newborn. The intracellular life cycle critical pathogenesis. Previous studies have established that major virulence factor , pore-forming toxin listeriolysin O (LLO), sufficient induce internalization into human...
The pore-forming toxin listeriolysin O (LLO) is a major virulence factor secreted by the facultative intracellular pathogen Listeria monocytogenes. This facilitates L. monocytogenes survival in macrophages and diverse nonphagocytic cells disrupting internalization vesicle, releasing bacterium into its replicative niche, cytosol. Neutrophils are innate immune that play an important role control of infections, yet it was unknown if LLO could confer advantage to neutrophils. We report can...
Host cell invasion is an indispensable step for a successful infection by intracellular pathogens. Recent studies identified pathogen-induced host plasma membrane perforation as novel mechanism used diverse pathogens (Trypanosoma cruzi, Listeria monocytogenes, and adenovirus) to promote their internalization into target cells. It was concluded that T. cruzi adenovirus damage the hijack endocytic-dependent resealing machinery, thereby invading cell. We studied L. monocytogenes its secreted...