A5025270666- Inflammasome and immune disorders
- Alzheimer's disease research and treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Asthma and respiratory diseases
- Autophagy in Disease and Therapy
- Vibrio bacteria research studies
- COVID-19 Clinical Research Studies
- Cystic Fibrosis Research Advances
- Antibiotic Resistance in Bacteria
- Cholesterol and Lipid Metabolism
- Parkinson's Disease Mechanisms and Treatments
- Cerebrospinal fluid and hydrocephalus
- Phagocytosis and Immune Regulation
- MicroRNA in disease regulation
- Heme Oxygenase-1 and Carbon Monoxide
- IL-33, ST2, and ILC Pathways
- interferon and immune responses
- Pancreatitis Pathology and Treatment
- Immune cells in cancer
- Erythrocyte Function and Pathophysiology
The Ohio State University
2020-2024
Significance We report the discovery of fundamental roles for noncanonical inflammasome molecule Caspase-4/11 in promoting pathological inflammatory and prothrombotic pathways severe acute respiratory syndrome coronavirus 2 (SARS–CoV-2) infections. Our work demonstrates that Caspase-11 has a broader role immune responses beyond its previously appreciated effects bacterial Further, we show Caspase-11–deficient mice infected with SARS–CoV-2 fare significantly better terms overall illness, lung...
Autophagy is a proposed route of amyloid-β (Aβ) clearance by microglia that halted in Alzheimer's Disease (AD), though mechanisms underlying this dysfunction remain elusive. Here, primary from adult AD (5xFAD) mice were utilized to demonstrate 5xFAD fail degrade Aβ and express low levels autophagy cargo receptor NBR1. In mouse brains, we show for the first time elevated microRNA cluster Mirc1/Mir17-92a, which known downregulate proteins. By situ hybridization post-mortem human tissue...
Alzheimer's disease (AD) is the sixth leading cause of death in USA. It established that neuroinflammation contributes to synaptic loss, neuronal death, and symptomatic decline AD patients. Accumulating evidence suggests a critical role for microglia, innate immune phagocytes brain. For instance, microglia release pro-inflammatory products such as IL-1β which highly implicated pathobiology. The mechanisms underlying transition proinflammatory promoters remain largely unknown. To address this...
Burkholderia cenocepacia (B. cenocepacia) is an opportunistic bacterium; causing severe life threatening systemic infections in immunocompromised individuals including cystic fibrosis patients. The lack of gasdermin D (GSDMD) protects mice against endotoxin lipopolysaccharide (LPS) shock. On the other hand, GSDMD promotes survival response to certain bacterial infections. However, role during B. infection not yet determined. Our vitro study shows that restricts replication within macrophages...
Cystic fibrosis (CF) human and mouse macrophages are defective in their ability to clear bacteria such as
Neuroinflammation and accumulation of Amyloid Beta (Aβ) accompanied by deterioration special memory are hallmarks Alzheimer's disease (AD). Effective preventative treatment options for AD still needed. Microglia in brains characterized elevated levels microRNA-17 (miR-17), which is defective autophagy, Aβ accumulation, increased inflammatory cytokine production. However, the effect targeting miR-17 on pathology loss not clear. To specifically inhibit microglia, we generated mannose-coated...
BackgroundMitochondria play a key role in immune defense pathways, particularly for macrophages. We and others have previously demonstrated that cystic fibrosis (CF) macrophages exhibit weak autophagy activity exacerbated inflammatory responses. Previous studies revealed mitochondria are defective CF epithelial cells, but to date, the connection between mitochondrial function macrophage dysregulation has not been fully elucidated. Here, we present characterization of dysfunction...
Abstract Alzheimer’s Disease (AD) is the 6th leading cause of death in US. It established that neuroinflammation contributes to synaptic loss, neuronal death, and symptomatic decline AD patients. Accumulating evidence suggests a critical role for microglia, innate immune phagocytes brain. For instance, microglia release pro-inflammatory products such as IL-1β which highly implicated pathobiology. The mechanisms underlying transition proinflammatory promoters remain largely unknown. To...
Abstract SARS-CoV-2 is a worldwide health concern, and new treatment strategies are needed 1 . Targeting inflammatory innate immunity pathways holds therapeutic promise, but effective molecular targets remain elusive. Here, we show that human caspase-4 (CASP4), its mouse homologue, caspase-11 (CASP11), upregulated in infections, CASP4 expression correlates with severity of infection humans. SARS-CoV-2-infected Casp11 -/- mice were protected from severe weight loss lung pathology, including...
An amendment to this paper has been published and can be accessed via a link at the top of paper.
Abstract Asthma is an inflammatory lung disorder characterized by mucus hypersecretion, cellular infiltration, and bronchial hyper-responsiveness. House dust mites (HDM) are the most prevalent cause of allergic sensitization. Canonical noncanonical inflammasomes multiprotein complexes that assemble in response to pathogen or danger-associated molecular patterns (PAMPs DAMPs). Murine caspase-11 engages inflammasome. We addressed role mediating host responses HDM subsequent inflammation using...