A5000365431- Inflammasome and immune disorders
- Immune Response and Inflammation
- Cystic Fibrosis Research Advances
- Asthma and respiratory diseases
- Vibrio bacteria research studies
- Cerebrospinal fluid and hydrocephalus
- Cell death mechanisms and regulation
- Epilepsy research and treatment
- Pancreatitis Pathology and Treatment
- Neuroscience and Neuropharmacology Research
- Alzheimer's disease research and treatments
- Erythrocyte Function and Pathophysiology
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- IL-33, ST2, and ILC Pathways
- Neuroinflammation and Neurodegeneration Mechanisms
- Antibiotic Resistance in Bacteria
- Ion channel regulation and function
- Phagocytosis and Immune Regulation
- Autophagy in Disease and Therapy
The Ohio State University
2019-2024
The Ohio State University Wexner Medical Center
2019-2024
Gout is characterized by attacks of arthritis with hyperuricemia and monosodium urate (MSU) crystal-induced inflammation within joints. Innate immune responses are the primary drivers for tissue destruction in gout. MSU crystals engage Nlrp3 inflammasome leading to activation caspase-1 production IL-1β IL-18 gout-affected joints, promoting influx neutrophils monocytes. Here we show that caspase-11-/- mice their derived macrophages produce significantly reduced levels gout-specific cytokines...
Autophagy is a proposed route of amyloid-β (Aβ) clearance by microglia that halted in Alzheimer's Disease (AD), though mechanisms underlying this dysfunction remain elusive. Here, primary from adult AD (5xFAD) mice were utilized to demonstrate 5xFAD fail degrade Aβ and express low levels autophagy cargo receptor NBR1. In mouse brains, we show for the first time elevated microRNA cluster Mirc1/Mir17-92a, which known downregulate proteins. By situ hybridization post-mortem human tissue...
Sudden unexpected death in epilepsy (SUDEP) is a fatal complication experienced by otherwise healthy patients. Dravet syndrome (DS) an inherited epileptic disorder resulting from loss of function the voltage-gated sodium channel, NaV 1.1, and associated with particularly high SUDEP risk. Evidence mounting that NaVs abundant brain also occur heart, suggesting very molecular mechanisms underlying could precipitate cardiac arrhythmias sudden death. Despite marked reduction 1.1 functional...
Burkholderia cenocepacia (B. cenocepacia) is an opportunistic bacterium; causing severe life threatening systemic infections in immunocompromised individuals including cystic fibrosis patients. The lack of gasdermin D (GSDMD) protects mice against endotoxin lipopolysaccharide (LPS) shock. On the other hand, GSDMD promotes survival response to certain bacterial infections. However, role during B. infection not yet determined. Our vitro study shows that restricts replication within macrophages...
Article21 October 2019Open Access Transparent process Caspase-11 counteracts mitochondrial ROS-mediated clearance of Staphylococcus aureus in macrophages Kathrin Krause Department Microbial Infection and Immunity, Infectious Diseases Institute, Ohio State University, Columbus, OH, USA Search for more papers by this author Kylene Daily Shady Estfanous Kaitlin Hamilton Asmaa Badr Arwa Abu Khweek Biology Biochemistry, Birzeit Birzeit, West Bank, Palestine Rana Hegazi Midhun NK Anne Brett Klamer...
Cystic fibrosis (CF) human and mouse macrophages are defective in their ability to clear bacteria such as
BackgroundMitochondria play a key role in immune defense pathways, particularly for macrophages. We and others have previously demonstrated that cystic fibrosis (CF) macrophages exhibit weak autophagy activity exacerbated inflammatory responses. Previous studies revealed mitochondria are defective CF epithelial cells, but to date, the connection between mitochondrial function macrophage dysregulation has not been fully elucidated. Here, we present characterization of dysfunction...
An amendment to this paper has been published and can be accessed via a link at the top of paper.
Abstract Asthma is an inflammatory lung disorder characterized by mucus hypersecretion, cellular infiltration, and bronchial hyper-responsiveness. House dust mites (HDM) are the most prevalent cause of allergic sensitization. Canonical noncanonical inflammasomes multiprotein complexes that assemble in response to pathogen or danger-associated molecular patterns (PAMPs DAMPs). Murine caspase-11 engages inflammasome. We addressed role mediating host responses HDM subsequent inflammation using...