A5000503729- Asthma and respiratory diseases
- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Inflammasome and immune disorders
- Virology and Viral Diseases
- T-cell and B-cell Immunology
- IL-33, ST2, and ILC Pathways
- Bone health and treatments
- Immune Response and Inflammation
- Respiratory viral infections research
- SARS-CoV-2 and COVID-19 Research
- Effects and risks of endocrine disrupting chemicals
- Hepatitis C virus research
- Inhalation and Respiratory Drug Delivery
- Immune Cell Function and Interaction
- Neurological diseases and metabolism
- Virus-based gene therapy research
- Listeria monocytogenes in Food Safety
- Essential Oils and Antimicrobial Activity
- RNA modifications and cancer
- Hereditary Neurological Disorders
- Vibrio bacteria research studies
- Viral Infectious Diseases and Gene Expression in Insects
- RNA Interference and Gene Delivery
- Veterinary Oncology Research
Nationwide Children's Hospital
2023-2025
The Ohio State University
2018-2021
University of Sadat City
2018-2021
Current efforts to develop Zika virus (ZIKV) subunit vaccines have been focused on pre-membrane (prM) and envelope (E) proteins, but the role of NS1 in ZIKV-specific immune response protection is poorly understood. Here, we an attenuated recombinant vesicular stomatitis (rVSV)-based vaccine expressing ZIKV prM-E-NS1 as a polyprotein. This vectored candidate mice, where single immunization induces antibody T cell responses that provide against challenge. Co-expression prM, E, significantly...
Multiple sclerosis (MS) is an immune-mediated chronic inflammatory and neurodegenerative disease of the central nervous system (CNS) affecting more than 2.5 million patients worldwide. Chronic demyelination in CNS has important role perpetuating axonal loss increases difficulty promoting remyelination. Therefore, regenerative, neuroprotective strategies are essential to overcome this impediment rescue integrity function. Neurotrophin 3 (NT-3) immunomodulatory anti-inflammatory properties,...
Significance We report that suppression of the serine protease elastase reshapes innate responses induced by injected vaccines containing alum adjuvant. This reprogramming improves induction protective antibodies in bloodstream and stimulates signals, which support development antibody mucosal tissues. Our findings identify as regulator blunts adjuvant activity alum. They also demonstrate vaccination via routes is not an absolute requirement for tissues secretions. Supplementation alum-based...
Human respiratory syncytial virus (RSV) is the leading cause of tract infections in humans. A well-known challenge development a live attenuated RSV vaccine that interferon (IFN)-mediated antiviral responses are strongly suppressed by nonstructural proteins which, turn, dampens subsequent adaptive immune responses. Here, we discovered novel strategy to enhance innate and immunity infection. Specifically, found recombinant RSVs deficient viral RNA N 6 -methyladenosine (m A) grown m...
Most Zika virus (ZIKV) vaccine research has focused on the E or prM-E proteins and induction of high levels neutralizing antibodies. However, these ZIKV antibodies cross-react with other flaviviruses, which may aggravate disease via an antibody-dependent enhancement (ADE) mechanism. NS1 protein be alternative antigen for development, since to do not bind virion, thereby eliminating risk ADE. Here, we show that recombinant VSV DNA vaccines expressing NS1, alone, confer partial protection...
Immune correlates of hepatitis C virus (HCV) clearance and control remain poorly defined due to the lack an informative animal model. We recently described acute chronic rodent HCV-like (RHV) infections in lab mice. Here, we developed MHC class I II tetramers characterize serial changes RHV-specific CD8 CD4 T cells during infection C57BL/6J RHV induced rapid expansion targeting viral structural nonstructural proteins. After clearance, virus-specific transitioned from effectors long-lived...
Abstract Background Osteoblastic bone metastasis represents the most common complication in men with prostate cancer (PCa). During progression and metastasis, PCa cells acquire properties similar to a phenomenon called osteomimicry, which promotes their ability metastasize, proliferate, survive microenvironment. The mechanism of osteomimicry resulting osteoblastic is unclear. Methods We developed characterized novel canine prostatic cell line (LuMa) that will be useful investigate...
It is well established that dendritic cells and macrophages play a role in antigen presentation to B T shaping cell responses via cytokines they produce. We have previously reported depletion of neutrophils improves the production mucosal IgA after sublingual immunization with Bacillus anthracis edema toxin as adjuvant. These past studies also demonstrated an inverse correlation exists between number by cells. Using specific inhibitors elastase, we addressed whether elastase activity...
Abstract Sublingual immunization is emerging as an alternative to nasal and induction of mucosal IgA responses. Using Bacillus anthracis edema toxin (EdTx) adjuvant, we previously showed that innate responses triggered after sublingual could limit generation We tested whether co-administration a neutrophil elastase inhibitor (NEI) rescue the ability EdTx induce broad antibody responses, including IgA. NEI supplementation vaccines containing promoted antigen-specific serum but also enhanced...
Abstract Charcot–Marie–Tooth Type 4C (CMT4C) is associated with mutations in the SH3 domain and tetratricopeptide repeats 2 (SH3TC2) gene, primarily expressed Schwann cells (SCs). Neurotrophin-3 (NT-3) an important autocrine factor for SC survival differentiation, it stimulates neurite outgrowth myelination. In this study, scAAV1.tMCK.NT-3 was delivered intramuscularly to 4-week-old Sh3tc2−/− mice, a model CMT4C, treatment efficacy assessed at 6-month post-gene delivery. Efficient transgene...
Abstract Paneth cells regulate many key aspects of gastrointestinal health through the antimicrobial products and cytokines they produce. Loss or defective cell functions leads to dysbiosis is one causes Inflammatory Bowel Disease (IBD). Mice with Sox9 gene deletion within intestinal epithelium (Sox9 ΔIEC mice) lack making these mice a great model for study IBD other consequences dysbiosis. Our separate studies have shown that gut microbiota could enhance allergic sensitization in subsequent...
Abstract Asthma is an inflammatory lung disorder characterized by mucus hypersecretion, cellular infiltration, and bronchial hyper-responsiveness. House dust mites (HDM) are the most prevalent cause of allergic sensitization. Canonical noncanonical inflammasomes multiprotein complexes that assemble in response to pathogen or danger-associated molecular patterns (PAMPs DAMPs). Murine caspase-11 engages inflammasome. We addressed role mediating host responses HDM subsequent inflammation using...
Abstract Zika virus (ZIKV) is a mosquito-borne flavivirus that can cause Congenital Syndrome (including microcephaly), Guillain-Barré syndrome, and other severe neurological disorders. Despite major efforts, there no FDA-approved vaccine for ZIKV. Current efforts to develop ZIKV subunit vaccines have been exclusively focused on pre-membrane (prM) envelope (E) proteins. However, the role of viral proteins such as nonstructural protein 1 (NS1) in protection poorly understood. Here, we...