A5012355893- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Virus-based gene therapy research
- Virology and Viral Diseases
- Respiratory viral infections research
- Viral Infections and Immunology Research
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Vectors
- Parvovirus B19 Infection Studies
- Proteoglycans and glycosaminoglycans research
- Plant Virus Research Studies
- Vector-borne infectious diseases
- Infection Control and Ventilation
- Silk-based biomaterials and applications
- Cell Adhesion Molecules Research
- RNA modifications and cancer
- Animal health and immunology
- Mosquito-borne diseases and control
- Heat shock proteins research
- HVDC Systems and Fault Protection
- Microbial infections and disease research
- Viral Infections and Outbreaks Research
The Ohio State University
2010-2022
Zhejiang A & F University
2018
Porcine epidemic diarrhea coronavirus (PEDV) has significantly damaged America's pork industry. Here we investigate the receptor usage and cell entry of PEDV. PEDV recognizes protein aminopeptidase N from pig human sugar coreceptor N-acetylneuraminic acid. Moreover, infects cells pig, human, monkey, bat. These results support idea bats as an evolutionary origin for PEDV, implicate a potential threat to other species, suggest antiviral strategies control its spread.
ABSTRACT A novel porcine deltacoronavirus (PdCV) was first discovered in Ohio and Indiana February 2014, rapidly spread to other states the United States Canada, caused significant economic loss swine industry. The origin virulence of this coronavirus are not known. Here, we characterized U.S. PdCV isolates determined their gnotobiotic conventional piglets. Genome analyses revealed that possess unique genetic characteristics share a close relationship with Hong Kong South Korean strains...
Gamma irradiation is a nonthermal processing technology that has been used for the preservation of variety food products. This shown to effectively inactivate bacterial pathogens. Currently, FDA approved doses up 4.0 kGy control food-borne pathogens in fresh iceberg lettuce and spinach. However, whether this dose range inactivates viruses less understood. We have performed systematic study on inactivation human norovirus surrogate (murine 1 [MNV-1]), virus-like particles (VLPs), vesicular...
Abstract N 6 -methyladenosine (m A) is the most prevalent internal modification of mRNAs in eukaryotes. Here we show that RNAs human respiratory syncytial virus (RSV) are modified by m A within discreet regions and these modifications enhance viral replication pathogenesis. Knockdown methyltransferases decreases RSV gene expression whereas knockdown demethylases has opposite effect. The G transcript contains modifications. Recombinant variants expressing transcripts lack particular clusters...
ABSTRACT Fresh produce is a major vehicle for the transmission of human norovirus (NoV) because it easily contaminated during both pre- and postharvest stages. However, ecology NoV in fresh poorly understood. In this study, we determined whether its surrogates can be internalized via roots disseminated to edible portions plant. The romaine lettuce growing hydroponic feed water were inoculated with 1 × 10 6 RNA copies/ml genogroup II genotype 4 (GII.4) strain or 2 PFU/ml animal caliciviruses...
Current efforts to develop Zika virus (ZIKV) subunit vaccines have been focused on pre-membrane (prM) and envelope (E) proteins, but the role of NS1 in ZIKV-specific immune response protection is poorly understood. Here, we an attenuated recombinant vesicular stomatitis (rVSV)-based vaccine expressing ZIKV prM-E-NS1 as a polyprotein. This vectored candidate mice, where single immunization induces antibody T cell responses that provide against challenge. Co-expression prM, E, significantly...
Human enteric viruses are among the main causative agents of shellfish-associated outbreaks. In this study, kinetics viral bioaccumulation in live oysters and heat stabilities predominant were determined both tissue culture oyster tissues. A human norovirus (HuNoV) GII.4 strain, HuNoV surrogates (murine [MNV-1], Tulane virus [TV]), hepatitis (HAV), rotavirus (RV) bioaccumulated to high titers within tissues, with different patterns for viruses. We tested thermal stability each at 62, 72,...
Human norovirus (HuNoV) is a major causative agent of food-borne gastroenteritis worldwide. Currently, there are no vaccines or effective therapeutic interventions for this virus. Development an attenuated vaccine HuNoV has been hampered by the inability to grow virus in cell culture. Thus, vector-based may be ideal. In study, we constructed recombinant vesicular stomatitis (rVSV-VP1) expressing VP1, capsid protein HuNoV. Expression VSV resulted formation virus-like particles (VLPs) that...
ABSTRACT One role of mRNA cap guanine-N-7 (G-N-7) methylation is to facilitate the efficient translation mRNA. The ribose 2′-O enigmatic, although recent work has implicated this as a signature avoid detection RNA by innate immune system (S. Daffis, K. J. Szretter, Schriewer, Q. Li, S. Youn, Errett, T. Y. Lin, Schneller, R. Zust, H. P. Dong, V. Thiel, G. C. Sen, Fensterl, W. B. Klimstra, Pierson, M. Buller, Gale, Shi, Diamond, Nature 468:452-456, 2010, doi:10.1038/nature09489). Working with...
The 5' cap methylation of viral RNA plays important roles in stability, efficient translation, and immune evasion. Thus, is an attractive target for antiviral discovery development new live attenuated vaccines. For coronaviruses, structure first methylated at the guanine-N-7 (G-N-7) position by nonstructural protein 14 (nsp14), which facilitates precedes subsequent ribose 2'-O nsp16-nsp10 complex. Using porcine epidemic diarrhea virus (PEDV), Alphacoronavirus, as a model, we showed that...
Human norovirus (HuNoV) is a leading cause of foodborne diseases worldwide. High-pressure processing (HPP) one the most promising nonthermal technologies for decontamination viral pathogens in foods. However, survival HuNoVs after HPP poorly understood because these viruses cannot be propagated vitro In this study, we estimated different HuNoV strains within genogroup II (GII) treatment using receptor-binding ability as an indicator. Four (one GII genotype 1 [GII.1] strain, two GII.4...
ABSTRACT Avian metapneumovirus (aMPV), also known as avian pneumovirus or turkey rhinotracheitis virus, is the causative agent of and associated with swollen head syndrome in chickens. Since its discovery 1970s, aMPV has been recognized an economically important pathogen poultry industry worldwide. The conserved region VI (CR VI) large (L) polymerase proteins paramyxoviruses catalyzes methyltransferase (MTase) activities that typically methylate viral mRNAs at guanine N-7 (G-N-7) ribose 2′-O...
The major inducible 70-kDa heat shock protein (hsp70) protects against measles virus (MeV) neurovirulence in the mouse that is caused by a cell-associated noncytolytic neuronal infection. Protection type I interferon (IFN) dependent, and we have established novel axis of antiviral immunity which hsp70 released from virus-infected neurons to induce IFN-β macrophages. present work used vesicular stomatitis (VSV) establish relevance hsp70-dependent fulminant cytopathic infections. In vitro, was...
Human norovirus (NoV) is responsible for over 90% of outbreaks acute nonbacterial gastroenteritis worldwide and accounts 60% cases foodborne illness in the United States. Currently, infectivity human NoVs poorly understood due to lack a cell culture system. In this study, we determined survival NoV genogroup II, genotype 4 (GII.4) strain seeded oyster homogenates after high-pressure processing (HPP) using novel receptor binding assay gnotobiotic pig model. Pressure conditions 350 MPa at 0°C...
Human norovirus (NoV) accounts for 95% of nonbacterial gastroenteritis worldwide. Currently, there is no vaccine available to combat human NoV as it not cultivable and lacks a small-animal model. Recently, we demonstrated that recombinant vesicular stomatitis virus (rVSV) expressing capsid protein (rVSV-VP1) induced strong immunities in mice (Y. Ma J. Li, Virol. 85:2942-2952, 2011). To further improve the safety efficacy candidate, heat shock 70 (HSP70) was inserted into rVSV-VP1 backbone...
ABSTRACT Human metapneumovirus (hMPV) is a member of the Pneumovirinae subfamily in Paramyxoviridae family that causes respiratory tract infections humans. Unlike members Paramyxovirinae subfamily, polymerase complex pneumoviruses requires an additional cofactor, M2-1 protein, which functions as transcriptional antitermination factor. The protein was found to incorporate zinc ions, although specific role(s) binding activity viral replication and pathogenesis remains unknown. In this study,...
Human norovirus (HuNoV) is responsible for more than 95% of outbreaks acute nonbacterial gastroenteritis worldwide. Despite major efforts, there are no vaccines or effective therapeutic interventions against this virus. Chicken immunoglobulin Y (IgY)-based passive immunization has been shown to be an strategy prevent and treat many enteric viral diseases. Here, we developed a highly efficient bioreactor generate high titers HuNoV-specific IgY in chicken yolks using recombinant vesicular...