A5022857536- Cardiac electrophysiology and arrhythmias
- Ion channel regulation and function
- Insect-Plant Interactions and Control
- Connexins and lens biology
- Animal Ecology and Behavior Studies
- Forest Insect Ecology and Management
- Cardiomyopathy and Myosin Studies
- Viral Infections and Immunology Research
- Atrial Fibrillation Management and Outcomes
- Wildlife Ecology and Conservation
- Cardiovascular Effects of Exercise
- Cardiac Arrhythmias and Treatments
- Insect Pest Control Strategies
- Plant and animal studies
- Electrochemical Analysis and Applications
- Cardiac pacing and defibrillation studies
- Amphibian and Reptile Biology
- Cardiovascular Function and Risk Factors
- Fluoride Effects and Removal
- Heart Failure Treatment and Management
- Species Distribution and Climate Change
- Nicotinic Acetylcholine Receptors Study
- Conservation, Ecology, Wildlife Education
- Plant Parasitism and Resistance
- Cardiac Ischemia and Reperfusion
Virginia Tech
2017-2025
Biomedical Research Institute
2021-2025
The Ohio State University Wexner Medical Center
2022-2024
The Ohio State University
2023-2024
Carilion Roanoke Memorial Hospital
2017-2024
University of Florida
2024
Lung Institute
2023
Roanoke College
2020
Ibero American University
2019
Carilion Clinic
2018
Computational modeling indicates that cardiac conduction may involve ephaptic coupling - intercellular communication involving electrochemical signaling across narrow extracellular clefts between cardiomyocytes. We hypothesized β1(SCN1B) -mediated adhesion scaffolds trans-activating NaV1.5 (SCN5A) channels within (<30 nm) perinexal adjacent to gap junctions (GJs), facilitating coupling. Super-resolution imaging indicated preferential β1 localization at the perinexus, where it co-locates with...
BACKGROUND: Viral cardiac infection represents a significant clinical challenge encompassing several etiological agents, disease stages, complex presentation, and resulting lack of mechanistic understanding. Myocarditis is major cause sudden death in young adults, where current knowledge the field dominated by later phases pathological immune responses. However, little known regarding how can acutely induce an arrhythmogenic substrate before Adenovirus leading myocarditis, but due to species...
Sudden unexpected death in epilepsy (SUDEP) is a fatal complication experienced by otherwise healthy patients. Dravet syndrome (DS) an inherited epileptic disorder resulting from loss of function the voltage-gated sodium channel, NaV 1.1, and associated with particularly high SUDEP risk. Evidence mounting that NaVs abundant brain also occur heart, suggesting very molecular mechanisms underlying could precipitate cardiac arrhythmias sudden death. Despite marked reduction 1.1 functional...
Aims: Atrial fibrillation (AF) is the most common sustained arrhythmia. Previous evidence in animal models suggests that gap junction (GJ) adjacent nanodomain – perinexus a site capable of independent intercellular communication via ephaptic transmission. Perinexal expansion associated with slowed conduction and increased ventricular arrhythmias models, but has not been studied human tissue. The purpose this study was to characterize humans determine if perinexal associates AF. Methods:...
Abstract The relationship between cardiac conduction velocity (CV) and extracellular potassium (K + ) is biphasic, with modest hyperkalemia increasing CV severe slowing CV. Recent studies from our group suggest that elevating sodium (Na calcium (Ca 2+ can enhance by an pathway parallel to gap junctional coupling (GJC) called ephaptic occur in the junction adjacent perinexus. However, it remains unknown whether these same interventions modulate as a function of K . We hypothesize Na , Ca GJC...
Les especes de Gastrolobium et d'Oxylobium (Leguminosae) du sud-ouest contiennent naturellement fluoroacetate (le sodium est utilise pour lutter contre les lagomorphes dingos). populations kangourous rats qui utilisent cette plante sont tres resistants au fluoroacetate. Il y a eu coevolution entre la production corps chez le developpement d'une resistance genetique animaux l'utilisent
We previously demonstrated that altering extracellular sodium (Na o ) and calcium (Ca can modulate a form of electrical communication between cardiomyocytes termed “ephaptic coupling” (EpC), especially during loss gap junction coupling. hypothesized Na Ca modulates conduction velocity (CV) arrhythmic burden ischemia. Electrophysiology was quantified by optically mapping Langendorff-perfused guinea pig ventricles with modified (147 or 155 mM) (1.25 2.0 30 min simulated metabolic ischemia (pH...
Cardiac conduction is understood to occur through gap junctions. Recent evidence supports ephaptic coupling as another mechanism of electrical communication in the heart. Conduction via junctions predicts a direct relationship between velocity (CV) and bulk extracellular resistance. By contrast, theory premised on existence biphasic CV volume specialized clefts within intercalated discs such perinexus. Our objective was determine ventricular structural changes micro- nanoscale spaces.
Cardiac voltage-gated sodium channel gain-of-function prolongs repolarization in the long-QT syndrome type 3 (LQT3). Previous studies suggest that narrowing perinexus within intercalated disc, leading to rapid depletion, attenuates LQT3-associated action potential duration (APD) prolongation. However, it remains unknown whether extracellular concentration modulates APD prolongation during gain-of-function. We hypothesized elevated and widened synergistically prolong LQT3. LQT3 was induced...
Recent studies revealed that relatively small changes in perfusate sodium ([Na+]o) composition significantly affect cardiac electrical conduction and stability contraction arrested ex vivo Langendorff heart preparations before during simulated ischemia. Additionally, [Na+]o modulates cardiomyocyte contractility via a sodium-calcium exchanger (NCX) mediated pathway. It remains unknown, however, whether modest to promote electrophysiologic similarly improve mechanical function baseline...
Abstract The uses of sodium monofluoroacetate (compound 1080) as a vertebrate predacide in the USA and Australia are reviewed. US applications centre on control native species, while many Australia's pests introduced species. This, coupled with natural resistance some Australian fauna to 1080, has contributed different patterns use both countries public attitudes programmes. 1080 case corrects omission pest examples from discussions school biology texts, highlights interplay economics,...
With the sudden increase in affordable manufacturing technologies, relationship between experimentalists and designing process for laboratory equipment is rapidly changing. While are still dependent on engineers manufacturers precision electrical, mechanical, optical equipment, it has become a realistic option house of other with less precise design requirements. This possible due to decreasing costs increasing functionality desktop three-dimensional (3-D) printers 3-D software. traditional...
The field metabolic rates and water influxes of Varanus acanthurus were determined by means doubly-labelled during late spring. mean rate was 0.101 +/- 0.032 mL CO2 g-1 h-1, which equivalent to an energy expenditure 63 kJ kg-1 day-1 a fresh food consumption 13.2 g day-1. influx 15.9 6.8 it is suggested that up 30% are via pulmo-cutaneous exchange drinking. It considered V. secretive 'sit wait' predator this accounts for the lower than predicted species.
BACKGROUND New onset atrial fibrillation (AF) in critically ill surgical patients is associated with significant morbidity and increased mortality. N-terminal pro-B type natriuretic peptide (NT-proBNP) released by cardiomyocytes response to stress may predict AF development after surgery. We hypothesized that elevated NT-proBNP level at intensive care unit (ICU) admission predicts a general trauma population. METHODS From July October 2015, concentrations were measured ICU admission....
Gap junctions (GJ) and connexins play integral roles in cellular physiology have been found to be involved multiple pathophysiological states from cancer cardiovascular disease. Studies over the last 60 years demonstrated utility of altering GJ signaling pathways experimental models, which has led them being attractive targets for therapeutic intervention. A number different mechanisms proposed regulate signaling, including channel blocking, enhancing open state, disrupting protein-protein...