A5013174323- Plant Pathogens and Resistance
- Plant Disease Resistance and Genetics
- Potato Plant Research
- Plant Virus Research Studies
- Insect Resistance and Genetics
- Plant Pathogens and Fungal Diseases
- Genetically Modified Organisms Research
- Chromosomal and Genetic Variations
- CRISPR and Genetic Engineering
- Plant tissue culture and regeneration
- Genetic diversity and population structure
- Genetic Mapping and Diversity in Plants and Animals
- Insect Pest Control Strategies
- Genetics and Plant Breeding
- Plant-Microbe Interactions and Immunity
- Entomopathogenic Microorganisms in Pest Control
- Insect and Pesticide Research
- Plant Reproductive Biology
- Nematode management and characterization studies
- Wheat and Barley Genetics and Pathology
- Photosynthetic Processes and Mechanisms
- Biotin and Related Studies
- Insect-Plant Interactions and Control
- Transgenic Plants and Applications
- Plant Pathogenic Bacteria Studies
Michigan State University
2016-2025
Plant (United States)
2021
Michigan United
2012-2018
Agricultural Research Council of South Africa
2010
Calvin University
2010
Donald Danforth Plant Science Center
2010
Institute of Entomology
2010
United States Department of Agriculture
2007
Agricultural Research Service
2007
University of Guelph
1999-2002
Significance Worldwide, potato is the third most important crop grown for direct human consumption, but breeders have struggled to produce new varieties that outperform those released over a century ago, as evidenced by widely North American cultivar (Russet Burbank) in 1876. Despite its importance, genetic diversity at whole-genome level remains largely unexplored. Analysis of cultivated and wild relatives using modern genomics approaches can provide insight into genomic extant germplasm,...
Genome editing using sequence-specific nucleases (SSNs) is rapidly being developed for genetic engineering in crop species. The utilization of zinc finger (ZFNs), transcription activator-like effector (TALENs) and CRISPR/Cas (clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated systems (Cas)) inducing double-strand breaks facilitates targeting virtually any sequence modification. Targeted mutagenesis via nonhomologous end-joining has been demonstrated...
The genome of potato, a major global food crop, was recently sequenced. work presented here details the integration potato reference (DM) with new sequence-tagged site marker-based linkage map and other physical genetic maps closely related species tomato. Primary anchoring DM assembly accomplished by use diploid segregating population, which genotyped several types molecular markers to construct ~936 cM comprising 2469 marker loci. In silico approaches used from genotype RH89-039-16 (RH)...
Genome-wide association studies (GWAS) are widely used in diploid species to study complex traits diversity and breeding populations, but GWAS software tailored autopolyploids is lacking. The objectives of this research were (i) develop an R package for based on the + mixed model, (ii) validate with simulated data, (iii) analyze a panel tetraploid potatoes. A unique feature package, called GWASpoly, its ability model different types polyploid gene action, including additive, simplex...
Abstract Background Current breeding approaches in potato rely almost entirely on phenotypic evaluations; molecular markers, with the exception of a few linked to disease resistance traits, are not widely used. Large-scale sequence datasets generated primarily through Sanger Expressed Sequence Tag projects available from limited number cultivars and access next generation sequencing technologies permits rapid data for additional cultivars. When coupled advent high throughput genotyping...
To facilitate genome-guided breeding in potato, we developed an 8303 Single Nucleotide Polymorphism (SNP) marker array using potato genome and transcriptome resources. validate the Infinium Potato Array, linkage maps from two diploid populations (DRH D84) compared these with assembled sequence. Both used doubled monoploid reference genotype DM1-3 516 R44 as female parent but had different heterozygous male parents (RH89-039-16 84SD22). Over 4,400 markers were mapped (1,960 DRH 2,454 D84, 787...
Genome editing using sequence-specific nucleases (SSNs) offers an alternative approach to conventional genetic engineering and opportunity extend the benefits of in agriculture. Currently available SSN platforms, such as zinc finger (ZFNs), transcription activator-like effector (TALENs), CRISPR/Cas (clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated systems (Cas)) have been used a range plant species for targeted mutagenesis via non-homologous end joining...
The third most important food crop worldwide, potato ( Solanum tuberosum L.) is a tetraploid outcrossing species propagated from tubers. Breeders have long been challenged by polyploidy, heterozygosity, and asexual reproduction. It has assumed that tetraploidy essential for high yield, the creation of inbred not feasible, propagation seed tubers ideal. In this paper, we question those assumptions propose to convert into diploid line–based true seed. Although conversion magnitude...
Clonally reproducing plants have the potential to bear a significantly greater mutational load than sexually species. To investigate this possibility, we examined breadth of genome-wide structural variation in panel monoploid/doubled monoploid clones generated from native populations diploid potato (Solanum tuberosum), highly heterozygous asexually propagated plant. As rare instances purely homozygous clones, they provided an ideal set for determining degree tolerated by species and deriving...
Cultivated potato is a clonally propagated autotetraploid species with highly heterogeneous genome. Phased assemblies of six cultivars including two chromosome-scale phased genome revealed extensive allelic diversity, altered coding and transcript sequences, preferential allele expression, structural variation that collectively result in complex transcriptome predicted proteome, which are distributed across the homologous chromosomes. Wild contribute to diversity tetraploid cultivars,...
Abstract Cultivated potato (Solanum tuberosum L.), a vegetatively propagated autotetraploid, has been bred for distinct market classes, including fresh market, pigmented, and processing varieties. Breeding efforts have relied on phenotypic selection of populations developed from intra- intermarket class crosses introgressions wild cultivated Solanum relatives. To retrospectively explore the effects breeding at genome level, we used 8303 single-nucleotide polymorphism markers to genotype...
The effects of selection on genome variation were investigated and visualized in tomato using a high-density single nucleotide polymorphism (SNP) array. 7,720 SNPs genotyped collection 426 accessions (410 inbreds 16 hybrids) over 97% the markers polymorphic entire collection. Principal component analysis (PCA) pairwise estimates F(st) supported that inbred represented seven sub-populations including processing, large-fruited fresh market, vintage, cultivated cherry, landrace, wild S....
Summary Cultivated potato ( Solanum tuberosum L.) is a highly heterozygous autotetraploid that presents challenges in genome analyses and breeding. Wild species serve as resource for the introgression of important agronomic traits into cultivated potato. One key chacoense diploid, inbred clone M6, which self‐compatible has desirable tuber market quality disease resistance traits. Sequencing assembly M6 S. generated an 825 767 562 bp 8260 scaffolds with N50 scaffold size 713 602 bp....
Potato is the world's most important vegetable crop, with nearly 400 million tons produced worldwide every year, lending to stability in food supply and socioeconomic impact. In general, potato an intensively managed requiring irrigation, fertilization, frequent pesticide applications order obtain highest yields possible. Important traits are easy find wild relatives of potato, but their introduction using traditional breeding can take 15–20 years. This due sexual incompatibility between...
Abstract As one of the world’s most important food crops, potato (Solanum tuberosum L.) has spurred innovation in autotetraploid genetics, including use SNP arrays to determine allele dosage at thousands markers. By combining genotype and pedigree information with phenotype data for economically traits, objectives this study were (1) partition genetic variance into additive vs. nonadditive components, (2) accuracy genome-wide prediction. Between 2012 2017, a training population 571 clones...
Potato breeding can be redirected to a diploid inbred/F1 hybrid variety strategy if self-compatibility introduced into germplasm. However, the majority of potato clones (Solanum spp.) possess gametophytic self-incompatibility that is primarily controlled by single multiallelic locus called S-locus which composed tightly linked genes, S-RNase (S-locus RNase) and multiple SLFs F-box proteins), are expressed in style pollen, respectively. Using genes known function Solanaceae SI mechanism, we...
The process of selecting superior performing plants for food, feed and fiber products dates back more than 10,000 years has been substantially refined in the last century. While perceived risks posed by genetically engineered crop extensively addressed, extant levels naturally occurring plant toxins food crops received far less attention. This review discusses how conventional breeding practices are used breeders to develop safe new varieties. Crops grouped into two categories: 1) with no...
Abstract Potato (Solanum tuberosum) is the third most important food crop in world. tubers must be stored at cold temperatures to minimize sprouting and losses due disease. However, strongly induce expression of potato vacuolar invertase gene (VInv) cause reducing sugar accumulation. This process, referred as “cold-induced sweetening,” a major postharvest problem for industry. We discovered that cold-induced VInv controlled by 200 bp enhancer, VInvIn2En, located its second intron. identified...
Potato (Solanum tuberosum) is a staple food crop and considered one of the main sources carbohydrates worldwide. Late blight (Phytophthora infestans) common scab (Streptomyces scabies) are two primary production constraints faced by potato farming. Previous studies have identified few resistance genes for both late scab; however, these explain only limited fraction heritability diseases. Genomic selection has been demonstrated to be an effective methodology breeding value prediction in many...
Genome-editing has revolutionized biology. When coupled with a recently streamlined regulatory process by the U.S. Department of Agriculture and potential to generate transgene-free varieties, genome-editing provides new avenue for crop improvement. For heterozygous, polyploid vegetatively propagated crops such as cultivated potato, Solanum tuberosum Group Tuberosum L., presents tremendous opportunities trait In traits improved resistance cold-induced sweetening, processing efficiency,...
Because of their adaptation to a diverse set habitats and stresses, wild species cultivated crops offer new sources genetic diversity for germplasm improvement. Using an Infinium array representing genome‐wide 8303 single nucleotide polymorphisms (SNPs), phylogenetic relationships allelic were evaluated within panel from Solanum sect. Petota . This consists 74 plant introductions (PIs) 25 provides representation tuber‐bearing germplasm. Unlike other molecular markers, SNPs have not been...
Abstract Knowledge regarding genetic diversity and population structure of breeding materials is essential for crop improvement. The Texas A&M University Potato Breeding Program has a collection advanced clones selected maintained in-vitro over 40-year period. Little known about its makeup usefulness the current program. In this study, 214 potato were genotyped with Infinium Illumina 22 K V3 Array. After filtering, total 10,106 single nucleotide polymorphic (SNP) markers used analysis....