A5102837479- Genetic Mapping and Diversity in Plants and Animals
- Plant Disease Resistance and Genetics
- Plant Pathogens and Resistance
- Wheat and Barley Genetics and Pathology
- Berry genetics and cultivation research
- Genetics and Plant Breeding
- Potato Plant Research
- Plant Pathogens and Fungal Diseases
- Plant Physiology and Cultivation Studies
- Genetic and phenotypic traits in livestock
- Nematode management and characterization studies
- Horticultural and Viticultural Research
- Plant Virus Research Studies
- Chromosomal and Genetic Variations
- Plant Gene Expression Analysis
- Crop Yield and Soil Fertility
- Plant nutrient uptake and metabolism
- Genetic diversity and population structure
- Entomopathogenic Microorganisms in Pest Control
- Soil Carbon and Nitrogen Dynamics
- Turfgrass Adaptation and Management
- Legume Nitrogen Fixing Symbiosis
- Yeasts and Rust Fungi Studies
- Plant and Fungal Species Descriptions
- Insect Resistance and Genetics
Biomathematics and Statistics Scotland
2014-2023
James Hutton Institute
2011-2023
Edinburgh Napier University
2017
University of Dundee
1998-2013
University of Birmingham
2007
Imperial College London
2002
Scottish Health Services
1992
Commonwealth Scientific and Industrial Research Organisation
1979
The genome of potato, a major global food crop, was recently sequenced. work presented here details the integration potato reference (DM) with new sequence-tagged site marker-based linkage map and other physical genetic maps closely related species tomato. Primary anchoring DM assembly accomplished by use diploid segregating population, which genotyped several types molecular markers to construct ~936 cM comprising 2469 marker loci. In silico approaches used from genotype RH89-039-16 (RH)...
Summary We hypothesized that plant exudates could either gel or disperse soil depending on their chemical characteristics. Barley ( Hordeum vulgare L. cv. Optic) and maize Zea mays Freya) root were collected using an aerated hydroponic method compared with chia Salvia hispanica L.) seed exudate, a commonly used exudate analogue. Sandy loam was passed through 500 ‐ μm mesh treated each at concentration of 4.6 mg g −1 dry soil. Two sets samples prepared. One set maintained 4°C to suppress...
Camellia sinensis is a beverage tree crop native to Southeast Asia and introductions have been made into several nonindigenous countries. No systematic assessment of genetic variability in tea has done anywhere. In this study, random amplified polymorphic DNA (RAPD) analysis was used estimate diversity taxonomic relationships 38 clones belonging the three varieties, assamica, sinensis, assamica ssp. lasiocalyx. Extensive detected between species, which partitioned within population...
New sequencing and genotyping technologies have enabled researchers to generate high density SNP genotype data for mapping populations. In polyploid species, usually contain a new type of information, the allele dosage, which is not used by current methodologies linkage analysis QTL mapping. Here we extend existing methodology use dosage on SNPs in an autotetraploid population. The dosages are inferred from intensity ratios using normal mixture models. steps (testing distorted segregation,...
We explored the use of genotyping by sequencing (GBS) on a recombinant inbred line population (GPMx) derived from cross between two-rowed barley cultivar 'Golden Promise' (ari-e.GP/Vrs1) and six-rowed 'Morex' (Ari-e/vrs1) to map plant height. identified three Quantitative Trait Loci (QTL), first in region encompassing spike architecture gene Vrs1 chromosome 2H, second an uncharacterised centromeric 3H, third 5H coinciding with previously described dwarfing Breviaristatum-e (Ari-e). Barley...
Thirty-nine genotypes of Hordeum spontaneum were selected from three geographically separated areas (southwestern, northern, and southeastern) the Fertile Crescent. The lines subject to AFLP (amplified fragment length polymorphism) analysis which a similarity matrix was produced. A dendrogram data showed, with two exceptions, that grouped together according area origin. This confirmed by principal coordinate in first southwestern other areas, turn could be second coordinate. While same site...
The effects of maize (Zea mays L.), genetically modified to express the Cry1Ab protein (Bt), and an insecticide on soil microbial faunal communities were assessed in a glasshouse experiment. Soil for experiment was taken from field sites where same cultivars grown allow comparison between results under conditions with those trials. Plants contrasting sandy loam clay soils, half sprayed pyrethroid (deltamethrin) samples at five-leaf stage, flowering, maturity. main effect all measured...
Consumption of raspberries promotes human health through intake pharmaceutically active antioxidants, including cyanidin and pelargonidin anthocyanins; products flavonoid metabolism also pigments conferring colour to fruit. Raspberry anthocyanin contents could be enhanced for nutritional quality benefits utilising DNA polymorphisms in modern marker assisted breeding. The objective was elucidate factors determining production these fruits. HPLC quantified eight glycosides: -3-sophoroside,...
Dense linkage maps derived by analysing SNP dosage in autotetraploids provide detailed information about the location of, and genetic model at, quantitative trait loci. Recent developments sequencing genotyping technologies enable researchers to generate high-density single nucleotide polymorphism (SNP) genotype data for mapping studies. For polyploid species, genotypes are informative allele dosage, Hackett et al. (PLoS ONE 8:e63939, 2013) presented theory how can be used map construction...
An earlier software application of ours, TetraploidMap for Windows, enabled linkage analysis and quantitative trait locus interval mapping to be carried out in an experimental cross autotetraploid species, using both dominant markers such as amplified fragment length polymorphisms codominant simple sequence repeats. The size was limited 800 markers, conducted each parent separately due the difficulties obtaining a reliable consensus map 2 parents. Modern genotyping technologies now give rise...
Abstract This article presents methodology for the construction of a linkage map in an autotetraploid species, using either codominant or dominant molecular markers scored on two parents and their full-sib progeny. The steps analysis are as follows: identification parental genotypes from offspring phenotypes; testing independent segregation markers; partition into groups cluster analysis; maximum-likelihood estimation phase, recombination frequency, LOD score all pairs same group EM...
Abstract Interval mapping of quantitative trait loci (QTL) for resistance to late blight, height, and maturity was performed on a tetraploid full-sib family potato comprising 227 clones from cross between susceptible parent, 12601ab1, resistant cultivar, Stirling, which were similar height main crop maturity. Thirty-eight AFLP primer combinations provided 585 informative markers, 23 SSRs proved useful identifying linkage groups (LGs). A simplex QTL allele found LGV Stirling close marker...
Statistical methods for mapping quantitative trait loci relative to genetic markers are now well established continuous traits with normal distributions. However, many of economic importance recorded on a discrete, ordinal scale. Here we describe model developed the analysis traits, such as degree difficulty in calving or categories plant disease resistance. The estimates distance from locus neighbouring markers, and also parameters, either gene effects an underlying scale probabilities...
Genetic resistance to barley leaf rust caused by Puccinia hordei involves both R genes and quantitative trait loci. The provide higher but less durable than the Consequently, exploring or partial has become a favorable alternative for controlling disease. Four loci have been identified in doubled haploid Steptoe (St)/Morex (Mx) mapping population. Further investigations are required study molecular mechanisms underpinning ultimately identify causal genes.We explored using genetical genomics...