A5084554370- Light effects on plants
- Photosynthetic Processes and Mechanisms
- Algal biology and biofuel production
- Plant Molecular Biology Research
- Potato Plant Research
- Plant nutrient uptake and metabolism
- Marine and coastal ecosystems
- Microbial Metabolic Engineering and Bioproduction
- Microbial Community Ecology and Physiology
- Protist diversity and phylogeny
- Plant Pathogens and Resistance
- Circadian rhythm and melatonin
- Lipid metabolism and biosynthesis
- Plant Disease Resistance and Genetics
- Plant Stress Responses and Tolerance
- Legume Nitrogen Fixing Symbiosis
- bioluminescence and chemiluminescence research
- Plant-Microbe Interactions and Immunity
- Enzyme Catalysis and Immobilization
- Microbial Metabolites in Food Biotechnology
- Plant biochemistry and biosynthesis
- Plant Gene Expression Analysis
- Plant and Biological Electrophysiology Studies
- Genetic diversity and population structure
- Aquatic Ecosystems and Phytoplankton Dynamics
Michigan State University
2013-2023
Michigan United
2012
Duke University
2011
University of California, San Diego
2011
Max Planck Society
2001-2008
Max Planck Institute of Molecular Plant Physiology
2000-2008
Scripps Research Institute
2005-2007
Transcriptional and allosteric regulation of ADP-Glc pyrophosphorylase (AGPase) plays a major role in the starch synthesis. Analysis response after detachment growing potato tubers from mother plant revealed that this concept requires extension. Starch synthesis was inhibited within 24 h tuber detachment, even though catalytic subunit AGPase (AGPB) overall activity remained high, substrates ATP Glc-1-P increased, glycerate-3-phosphate/inorganic orthophosphate (the activator inhibitor,...
Unicellular marine algae have promise for providing sustainable and scalable biofuel feedstocks, although no single species has emerged as a preferred organism. Moreover, adequate molecular genetic resources prerequisite the rational engineering of algal feedstocks are lacking most candidate species. Heterokonts genus Nannochloropsis naturally high cellular oil content already in use industrial production high-value lipid products. First success applying reverse genetics by targeted gene...
The C-Repeat Binding Factor (CBF) cold-response pathway has a prominent role in cold acclimation, the process whereby certain plants increase tolerance to freezing response low nonfreezing temperatures. In Arabidopsis, CBF is characterized by rapid induction of 1 (CBF1), CBF2, and CBF3 genes, which encode transcriptional activators, followed CBF-targeted genes known as "CBF regulon." Expression regulon results an tolerance. Previous studies established that CBF1, are subject circadian...
Significance Worldwide, potato is the third most important crop grown for direct human consumption, but breeders have struggled to produce new varieties that outperform those released over a century ago, as evidenced by widely North American cultivar (Russet Burbank) in 1876. Despite its importance, genetic diversity at whole-genome level remains largely unexplored. Analysis of cultivated and wild relatives using modern genomics approaches can provide insight into genomic extant germplasm,...
Abstract The compartmentation of metabolism in heterotrophic plant tissues is poorly understood due to the lack data on metabolite distributions and fluxes between subcellular organelles. main reason for this suitable experimental methods with which intracellular can be measured. Here, we describe a nonaqueous fractionation method that allows metabolites developing potato (Solanum tuberosum L. cv Desiree) tubers calculated. In addition, have coupled recently described gas chromatography-mass...
Abstract The pseudoresponse regulators (PRRs) participate in the progression of circadian clock Arabidopsis thaliana. founding member family, TIMING OF CAB EXPRESSION1 (TOC1), is an essential component transcriptional network that constitutes core mechanism oscillator. Recent data suggest a role regulation for all five members PRR family; however, molecular function TOC1 or any other PRRs remains unknown. In this work, we present evidence involvement PRR3 protein stability. was temporally...
Up to 30% of the plant transcriptome is circadian clock-regulated in different species; however, we still lack a good understanding mechanisms involved these genome-wide oscillations gene expression. Here, show that PSEUDO-RESPONSE REGULATOR 7 (PRR7), central component Arabidopsis clock, directly repression master regulators growth, light signaling and stress responses. The expression levels most PRR7 target genes peak around dawn, an antiphasic manner protein levels, were repressed by PRR7....
Nannochloropsis oceanica is an oleaginous microalga rich in ω3 long-chain polyunsaturated fatty acids (LC-PUFAs) content, the form of eicosapentaenoic acid (EPA). We identified enzymes involved LC-PUFA biosynthesis N. CCMP1779 and generated multigene expression vectors aiming at increasing content vivo. isolated cDNAs encoding four desaturases (FAD) determined their function by heterologous S. cerevisiae. To increase multiple CCMP1779, we developed a genetic engineering toolkit that includes...
PSEUDO-RESPONSE REGULATORs (PRRs) play overlapping and distinct roles in maintaining circadian rhythms regulating diverse biological processes, including the photoperiodic control of flowering, growth, abiotic stress responses. PRRs act as transcriptional repressors associate with chromatin via their conserved C-terminal CCT (CONSTANS, CONSTANS-like, TIMING OF CAB EXPRESSION 1 [TOC1/PRR1]) domains by a still-poorly understood mechanism. Here, we identified genome-wide targets PRR9 using...
Utilization of microalgae has been hampered by limited tools for creating loss-of-function mutants. Furthermore, modified strains deployment into the field must be free antibiotic resistance genes and face fewer regulatory hurdles if they are transgene free. The oleaginous microalga, Nannochloropsis oceanica CCMP1779, is an emerging model microalgal lipid metabolism. We present a one-vector episomal CRISPR/Cas9 system N. that enables generation marker-free mutant lines. CEN/ARS6 region from...
Photosynthetic microalgae are considered a viable and sustainable resource for biofuel feedstocks, because they can produce higher biomass per land area than plants be grown on non-arable land. Among many production, Nannochloropsis oceanica (CCMP1779) is particularly promising, following nutrient deprivation it produces very high amounts of triacylglycerols (TAG). The committed step in TAG synthesis catalyzed by acyl-CoA:diacylglycerol acyltransferase (DGAT). Remarkably, total 13 putative...
Summary Interlocking transcriptional loops and regulated protein degradation are the principal mechanisms involved in generation of self‐sustaining circadian rhythms many organisms. In Arabidopsis first proposed regulatory loop transcription factors CIRCADIAN CLOCK ASSOCIATED ( CCA1 ) LATE ELONGATED HYPOCOTYL LHY pseudo‐response regulator TIMING OF CHLOROPHYLL A/B BINDING PROTEIN TOC1 / PRR1 ). Recent findings indicate that homologues PRR7 PRR9 might also be with . this study we show...
Nannochloropsis oceanica CCMP1779 is a marine unicellular stramenopile and an emerging reference species for basic research on oleogenic microalgae with biotechnological relevance. We investigated its physiology transcriptome under light/dark cycles. observed oscillations in lipid content predominance of cell division the first half dark phase. Globally, more than 60% genes cycled N. CCMP1779, gene expression peaking at different times day. Interestingly, phase involved certain biological...
The marine microalgae Nannochloropsis oceanica (CCMP1779) is a prolific producer of oil and considered viable sustainable resource for biofuel feedstocks. Nitrogen (N) availability has strong impact on the physiological status metabolism microalgal cells, but exact nature this response poorly understood. To fill gap we performed transcriptomic profiling combined with cellular molecular analyses N. CCMP1779 during transition from quiescence to autotrophy. N deprivation-induced was accompanied...
Natural light environments are highly variable. Flexible adjustment between energy utilization and photoprotection is therefore of vital importance for plant performance fitness in the field. Short-term reactions to changing intensity triggered inside chloroplasts leaves within seconds minutes, whereas long-term adjustments proceed over hours days, integrating multiple signals. While mechanisms acclimation have been studied by constant growth during day, responses fluctuating rarely...
Summary Nannochloropsis species, unicellular industrial oleaginous microalgae, are model organisms for microalgal systems and synthetic biology. To facilitate community‐based annotation mining of the rapidly accumulating functional genomics resources, we have initiated an international consortium present a comprehensive multi‐omics resource database named Design Synthesis (NanDeSyn; http://nandesyn.single‐cell.cn ). Via Tripal toolkit, it features user‐friendly interfaces hosting genomic...
In Arabidopsis , the circadian clock regulates UV-B-mediated changes in gene expression. Here it is shown that components are able to inhibit UV-B-induced expression a gene-by-gene-specific manner and act downstream of initial UV-B sensing by COP1 (CONSTITUTIVE PHOTOMORPHOGENIC 1) UVR8 (UV RESISTANCE LOCUS 8). For example, induction ELIP1 ( EARLY LIGHT INDUCIBLE PROTEIN 1 ) PRR9 PSEUDO-RESPONSE REGULATOR 9 directly regulated LUX (LUX ARRYTHMO), ELF4 (EARLY FLOWERING 4), ELF3. Moreover,...