A5103189528- Algal biology and biofuel production
- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Enzyme Catalysis and Immobilization
- Photosynthetic Processes and Mechanisms
- biodegradable polymer synthesis and properties
- Biodiesel Production and Applications
- Aquatic Ecosystems and Phytoplankton Dynamics
- Membrane Separation Technologies
- Microplastics and Plastic Pollution
- Catalysis and Hydrodesulfurization Studies
- Marine and coastal ecosystems
- Membrane-based Ion Separation Techniques
- Biopolymer Synthesis and Applications
- Catalysis for Biomass Conversion
- Microbial Metabolites in Food Biotechnology
- Viral Infectious Diseases and Gene Expression in Insects
- Enzyme Production and Characterization
- Process Optimization and Integration
- Innovative Microfluidic and Catalytic Techniques Innovation
- Electrohydrodynamics and Fluid Dynamics
- Fungal and yeast genetics research
- Extraction and Separation Processes
- 3D Printing in Biomedical Research
- Advanced Control Systems Optimization
Korea Advanced Institute of Science and Technology
2015-2024
Hanjiang Normal University
2024
Anyang Normal University
2024
Houston Methodist Sugar Land Hospital
2024
Advanced Biomass R&D Center (South Korea)
2015-2023
Daejeon University
2017-2018
GS Caltex (South Korea)
2015
Schlumberger (British Virgin Islands)
2008-2014
Washington Center
2014
University of Washington
2014
Abstract Genome editing is crucial for genetic engineering of organisms improved traits, particularly in microalgae due to the urgent necessity next generation biofuel production. The most advanced CRISPR/Cas9 system simple, efficient and accurate some organisms; however, it has proven extremely difficult including model alga Chlamydomonas . We solved this problem by delivering Cas9 ribonucleoproteins (RNPs) comprising protein sgRNAs avoid cytotoxicity off-targeting associated with...
Abstract Alcaligenes eutrophus NCIMB 11599 was cultivated to produce poly(3‐hydroxybutyric acid) (PHB) from glucose by the automatic fed‐batch culture technique. The concentration of broth controlled at 10 20 g/L two methods: using exit gas data obtained a mass spectrometer and an on‐line analyzer. effect ammonium limitation on PHB synthesis different phases studied. final cell concentration, productivity increased as ammonia feeding stopped higher concentration. High concentrations (121...
Optimization was carried out for the recovery of microbiol poly(3-hydroxybutyrate) (PHB) from Alcaligenes eutrophus. This process involved use a dispersion made sodium hypochlorite solution and chloroform. The enabled us to take advantage both differential digestion by solvent extraction PHB (%) cell powder maximized using 30% concentration, 90-min treatment time, 1:1 (v/v) chloroform-to-aqueous-phase ratio. Under these optimal conditions, about 91% purity recovered higher than 97%. number...
Microalgae are considered promising alternative energy sources because they consume CO2 and accumulate large amounts of lipids that can be used as biofuel. Nannochloropsis is a particularly microalga due to its high growth rate lipid content, the availability genomic information. Transcription factors (TFs) global regulators biological pathways by up- or down-regulation related genes. Among these, basic helix-loop-helix (bHLH) TFs regulate growth, development, stress responses in plants...
Microalgae are considered as excellent platforms for biomaterial production that can replace conventional fossil fuel-based fuels and chemicals. Genetic engineering of microalgae is prerequisite to maximize materials reduce costs the production. Transcription factors (TFs) emerging key regulators metabolic pathways enhance molecules biofuels other materials. TFs with basic leucine zipper (bZIP) domain have been known stress associated lipid metabolism in plants. We overexpressed a bZIP TF,...
Genetic engineering of microalgae is necessary to produce economically feasible strains for biofuel production. Current efforts are focused on the manipulation individual metabolic genes, but outcomes not sufficiently stable and/or efficient large-scale production biofuels and other materials. Transcription factors (TFs) emerging as good alternatives microalgae, only increase biomaterials enhance stress tolerance. Here, we investigated an AP2 type TF Wrinkled1 in Arabidopsis (AtWRI1) known a...
Lignocellulosic raw materials have extensively been examined for the production of bio-based fuels, chemicals, and polymers using microbial platforms. Since xylose is one major components hydrolyzed lignocelluloses, it being considered a promising substrate in lignocelluloses based fermentation process. Ralstonia eutropha, most powerful natural producers polyhydroxyalkanoates (PHAs), has polymers. However, to best our knowledge, lignocellulosic feedstock not employed R. eutropha probably due...
A dibenzothiophene (DBT)-desulfurizing bacterial strain was isolated and identified as Gordona CYKS1. Strain CYKS1 found to transform DBT 2-hydroxybiphenyl via the 4S pathway be able also use organic sulfur compounds other than a sole source. Its desulfurization activity susceptible sulfate repression. Active resting cells for could prepared only in early growth phase. When two types of diesel oils, middle distillate unit feed (MDUF) light gas oil (LGO) containing various including DBT, were...
We studied recovery of poly(3-hydroxybutyric acid) (PHB) from Alcaligenes eutrophus and a recombinant Escherichia coli strain harboring the A. poly(3-hydroxyalkanoic biosynthesis genes. The amount PHB degraded to lower-molecular-weight compound in during process was significant when sodium hypochlorite used, but E. negligible. However, there no difference between two microorganisms patterns molecular weight change recovered by using dispersions solution chloroform. To understand these...
Abstract This study describes the microstructure and thermal mechanical properties of poly(hydroxybutyrate‐co‐hydroxyvalerate) (PHB/HV)–organoclay nanocomposites prepared by melt intercalation using Cloisite 30B, a monotallow bis‐hydroxyethyl ammonium‐modified montmorillonite clay. X‐ray diffractometry transmission electron microscopy analyses clearly confirm that an intercalated is formed finely distributed in PHB/HV copolymer matrix because has strong hydrogen bond interaction with...
It is important to produce L(+)-lactic acid at the lowest cost possible for lactic become a candidate monomer material promising biodegradable polylactic acid. In an effort develop high-rate bioreactor that provides high productivity along with concentration of acid, performance membrane cell-recycle (MCRB) was investigated via experimental studies and simulation optimization. Due greatly increased cell density, productivity, 21.6 g L−1 h−1, obtained in reactor. The concentration, however,...