A5100360844- Algal biology and biofuel production
- Korean Urban and Social Studies
- Aquatic Ecosystems and Phytoplankton Dynamics
- Photosynthetic Processes and Mechanisms
- Trace Elements in Health
- Enzyme Catalysis and Immobilization
- Enzyme Production and Characterization
- Microbial Metabolic Engineering and Bioproduction
- Heavy Metal Exposure and Toxicity
- Enzyme Structure and Function
- Toxic Organic Pollutants Impact
- Protein Hydrolysis and Bioactive Peptides
- Prion Diseases and Protein Misfolding
- Water Quality Monitoring and Analysis
- Chromium effects and bioremediation
- Iron Metabolism and Disorders
- Synthetic Organic Chemistry Methods
- Amino Acid Enzymes and Metabolism
- Biochemical and Molecular Research
- Aluminum toxicity and tolerance in plants and animals
- Evolution and Genetic Dynamics
- CRISPR and Genetic Engineering
- Electrochemical sensors and biosensors
- Urbanization and City Planning
- Carbohydrate Chemistry and Synthesis
Korea Advanced Institute of Science and Technology
1996-2019
Advanced Biomass R&D Center (South Korea)
2015-2019
Catholic University of Korea
1999
Abstract Genome editing is crucial for genetic engineering of organisms improved traits, particularly in microalgae due to the urgent necessity next generation biofuel production. The most advanced CRISPR/Cas9 system simple, efficient and accurate some organisms; however, it has proven extremely difficult including model alga Chlamydomonas . We solved this problem by delivering Cas9 ribonucleoproteins (RNPs) comprising protein sgRNAs avoid cytotoxicity off-targeting associated with...
Genetic engineering of microalgae is necessary to produce economically feasible strains for biofuel production. Current efforts are focused on the manipulation individual metabolic genes, but outcomes not sufficiently stable and/or efficient large-scale production biofuels and other materials. Transcription factors (TFs) emerging as good alternatives microalgae, only increase biomaterials enhance stress tolerance. Here, we investigated an AP2 type TF Wrinkled1 in Arabidopsis (AtWRI1) known a...
Four mutations—R778L, A874V, L1083F, and 2304delC—in the copper-transporting enzyme, P-type ATPase (ATP7B), were identified in Korean Patients with Wilson disease. Arg778Leu, most frequently reported mutation of this was found six eight unrelated patients studied, an allele frequency 37.5%, which is considerably higher than those other Asian populations. The novel single nucleotide deletion, 2304delC, one patient. Since a at cDNA 2302 (2302insC) had been previously described, region ATP7B...
Oleaginous microalgae of the Nannochloropsis genus are considered excellent candidates for biofuels and value-added products owing to their high biomass productivity lipid content. Here, we report first overexpression detection a heterologous sfCherry fluorescent protein in salina order develop transformation toolbox future genetic improvements. Particle bombardment was employed transformation, expression Shble under control TUB UEP promoters, cloned from N. salina, used confer resistance...
Abstract Microalgae are promising feedstocks for sustainable and eco‐friendly production of biomaterials, which can be improved by genetic engineering. It is also necessary to optimize the processes produce biomaterials from engineered microalgae. We previously reported that improvements an industrial microalga Nannochloropsis salina overexpressing a basic helix‐loop‐helix transcription factor (NsbHLH2). These transformants showed growth lipid particularly during early phase culture under...
A lipase gene, lipK, and a modulator limK, of Pseudomonas sp. strain KFCC 10818 have been cloned, sequenced, expressed in Escherichia coli. The limK gene is located immediately downstream the lipK gene. Enzymatically active was produced only presence effect LimK on expression similar to those subfamily I.1 I.2 lipase-specific foldases (Lifs). deduced amino acid sequence shares low homology (17 19%) with known Lifs, suggesting that distantly related species. Surprisingly, variant does not...
Wilson disease (WD), an inherited disorder affecting copper metabolism, is characterized by hepatic cirrhosis and neuronal degeneration, which result from toxic levels of that accumulate in the liver brain, respectively. We reported previously ≈ 1.3‐kb promoter WD gene contains four metal response elements (MREs). Among MREs, MREa plays most important role transcriptional activation promoter. Electrophoretic mobility shift assays (EMSAs) using synthetic oligonucleotide containing binding...
Production of functional biocompounds from microalgae garnered interests different industrial sectors; however, the overall productivity these is required to be substantially improved realize their commercialization. Herein, laboratory adaptation Scenedesmus deserticola was performed using glucose as an organic carbon source with aim improving its heterotrophic capability and production potential loliolide. Serial S. on agar plates led selecting HS4 high loliolide potential; subjected...
국가균형발전정책은 한국 국토정책의 중요한 목표 중 하나다. 세종시 건설과 공공기관 이전 등이 추진되었지만, 수도권과 비수도권의 격차는 오히려 확대되고 있다. 저출산과 함께 지방의 인구가 감소하면서 지방소멸론도 대두되고 격차 극복을 목적으로 하는 한국의 프랑스 국토균형발전정책의 경험에서 정책적 시사점을 얻을 수 프랑스는 1940년대부터 국토균형발전정책을 추진해 왔기 때문이다. 국가균형발전은 국가가 포기할 없는 전략적 가치이다. 공간 불평등은 소득 불평등과 마찬가지로 공공이 해결해야 시장실패이기 본 연구의 목적은 프랑스의 경험을 분석하여 국가균형발전정책에 대한 도출하는 것이다. 국가균형발전정책의 시사점은 다음과 같다. 첫째, 일관성과 지속성을 유지하면서 지방분권에 기반한 국가균형발전정책을 추진해야 한다. 둘째, 특정 지역의 발전이 아니라 전 국토의 발전을 목표로 하고 낙후지역에 지원은 특히 강화해야 셋째, 정책효과를 높이기 위해 객관적이고 투명한 다양한 지표를 개발하여 국토...