A5023485682- CRISPR and Genetic Engineering
- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant Virus Research Studies
- Plant Gene Expression Analysis
- Diverse Approaches in Healthcare and Education Studies
- RNA and protein synthesis mechanisms
- Chromosomal and Genetic Variations
- Insect symbiosis and bacterial influences
- Microbial Metabolic Engineering and Bioproduction
- Innovation in Digital Healthcare Systems
- Education and Learning Interventions
- Banana Cultivation and Research
- Electrocatalysts for Energy Conversion
- Plant Stress Responses and Tolerance
- Algal biology and biofuel production
- Flowering Plant Growth and Cultivation
- HVDC Systems and Fault Protection
- Plant Physiology and Cultivation Studies
- Enzyme Catalysis and Immobilization
- Innovation and Socioeconomic Development
- Evolution and Genetic Dynamics
- Photosynthetic Processes and Mechanisms
- Education, Safety, and Science Studies
- Cancer-related gene regulation
Seoul National University
2002-2024
Sogang University
2022
Institute for Basic Science
2016-2021
Seoul National University of Science and Technology
2021
University of Wisconsin–Madison
2008-2015
Abstract Genome editing is crucial for genetic engineering of organisms improved traits, particularly in microalgae due to the urgent necessity next generation biofuel production. The most advanced CRISPR/Cas9 system simple, efficient and accurate some organisms; however, it has proven extremely difficult including model alga Chlamydomonas . We solved this problem by delivering Cas9 ribonucleoproteins (RNPs) comprising protein sgRNAs avoid cytotoxicity off-targeting associated with...
As the most abundant internal modification of mRNA, N6 -methyladenosine (m6 A) methylation RNA is emerging as a new layer epitranscriptomic gene regulation in cellular processes, including embryo development, flowering-time control, microspore generation and fruit ripening, plants. However, role m6 A plant responses to environmental stimuli remains largely unexplored. In this study, we show that plays an important salt stress tolerance Arabidopsis. All mutants writer components, MTA, MTB,...
Abstract In the winter-annual accessions of Arabidopsis thaliana, presence an active allele FRIGIDA (FRI) elevates expression FLOWERING LOCUS C (FLC), a repressor flowering, and thus confers vernalization requirement. FLC activation by FRI involves methylation Lys 4 histone H3 (H3K4) at chromatin. Many multicellular organisms that have been examined contain two classes H3K4 methylases, yeast (Saccharomyces cerevisiae) Set1 class related to Drosophila melanogaster Trithorax. this work, we...
The epigenetic regulation of the floral repressor Flowering Locus C (FLC) is one critical factors that determine flowering time in Arabidopsis thaliana. Although many FLC regulators, and their effects on chromatin, have been extensively studied, resetting has not yet thoroughly characterized. Here, we investigate expression during gametogenesis embryogenesis using FLC::GUS transgenic plants RNA analysis. Regardless state adult plants, disappeared gametophytes. Subsequently, was reactivated...
The winter-annual habit of Arabidopsis thaliana requires active alleles flowering locus C (FLC), which encodes a potent repressor, and FRIGIDA (FRI), an activator FLC. FLC activation by FRI is accompanied increase in specific histone modifications, such as tri-methylation H3 at lysine 4 (H3K4me3), three H3K4 methyltransferases, the Drosophila Trithorax-class trithorax1 (ATX1) ATX2, yeast Set1-class ATX-related7/set domain group25 (ATXR7/SDG25). However, lesions all these genes failed to...
Significance Proper timing of flowering is a key factor in reproductive success. Certain plant species require prolonged cold exposure during winter to flower the spring; however, how plants measure duration not well understood. In this study, we describe identification methyltransferase that required for proper measurement exposure.
Abstract CRISPR/Cas9-mediated genome editing is an important and versatile technology in modern biological research. Recent advancements include base-editing CRISPR tools that enable targeted nucleotide substitutions using a fusion protein comprising nickase variant of Cas9 base deaminase. Improvements efficiencies inheritable edited loci need to be made make viable system plants. Here, we report efficiency cytosine editors (CBEs) Arabidopsis thaliana by applying the strong endogenous RPS5a...
Vernalization, the promotion of flowering in response to low temperatures, is one best characterized examples epigenetic regulation plants. The proportional duration cold period, but mechanism by which plants measure time at temperatures has been a long-standing mystery. We show that quantitative induction first gene Arabidopsis vernalization pathway, VERNALIZATION INSENSITIVE 3 (VIN3), regulated components Polycomb Response Complex 2, trimethylates histone H3 lysine 27 (H3K27me3). In...
Abstract Recently, new types of base editors, C-to-G editors (CGBEs), that enable cytosine transversions are unachievable with (CBEs) and adenosine (ABEs), have been developed in human cells. However, despite their importance crop genome editing, the efficacy CGBEs has not yet extensively evaluated. In our study, based on previously reported plant-compatible CBE CGBE, we demonstrated monocot (PcCGBEs) (C-to-G) rice protoplasts. For all targets tested, PcCGBEs (monocot CGBEs) appeared to...
The floral regulatory gene SUPERMAN (SUP) encodes a C2H2 type zinc finger protein that is required for maintaining boundaries between organs in Arabidopsis. It has been proposed the main function of SUP to balance cell proliferation third and fourth whorl developing flowers, thereby two whorls. To gain further insight into SUP, we have ectopically expressed using promoter APETALA1 (AP1), initially throughout meristems later becomes restricted first second Flowers AP1::SUP plants fewer...
The demand for vegetable oil, which is mainly used dietary purposes and cooking, steadily increasing worldwide. It often desirable to reduce unsaturation levels of fatty acids in order increase storage stability trans-fat generation during cooking. Functional disruption FATTY ACID DESATURASE 2 (FAD2) prevents the conversion monounsaturated oleic acid polyunsaturated linoleic acid, thereby enhancing production acid. However, FAD2 null alleles, due growth defects under stress conditions, are...
신종 코로나 바이러스 감염증이 전 세계적으로 확산한 2020년을 기점으로 비대면 문화가 가속화되면서 대규모의 인프라를 소유한 사업자로부터 합리적인 비용으로 언제 어디서든 인터넷을 통해 데이터의 연결, 교환, 저장이 가능한 가상화된 컴퓨팅 리소스를 제공하는 서비스로 접근한 후, 해당 필요한 만큼 빌려서 사용한 뒤 비용을 지불하는 방식인 클라우드 서비스에 대한 수요가 폭증하고 대부분의 조직은 이러한 기반 환경으로 완전히 전환하거나 전환을 고려하고 있다. 이런 시대적 흐름 속에서 사용자 측면의 기술 수용 연구는 필연적이다. 본 논문은 아마존의 퍼블릭 서비스인 AWS의 가상 서버(Instance) 생성 절차를 중심으로 소비자의 이용이라는 맥락에 초점을 맞춘 모형인 확장된 통합기술수용모형(UTAUT2)을 사용하여 사용자의 수용에 어떤 변인들이 영향을 미치는지 측정 모형을 수립, 검증하고 직접적인 영향력이 발견되지 않은 변인의 추가 경로를 탐색하여 최종 도출하였다. 이를 기술과 조직...
자연어 처리 기술의 발달로 AI 스피커에서 수행할 수 있는 기능이 확대 되었음에도 불구하고 음성인식 기술은 아직 미흡한 부분이 있으며 사용자들은 자연스러운 대화가 안 되는 등 일상 사용 환경에서의 불편을 경험한다. 이러한 대화의 음성인식이 어려운 주요 이유 중 하나는 “음…”, “어…” 등의 간투사와 같은 비정형 발성에 기인한다. 배경을 토대로 본 연구는 스피커 환경에서 간투사 시 스피커의 반응 대기 시간 조절(1초, 2초, 3초, 4초, 5초, 6초, 7초)과 방법(피드백 없음, 빛 피드백, 빛과 음성 피드백)이 사용자 경험에 어떠한 차이를 보이는지, 또 사용자가 가장 적정하다고 생각하는 길이가 무엇인지 알아보고자 하였다. 실험은 프로토타입을 제작하여 오즈의 마법사(Wizard of Oz)방식으로 총 50명을 대상으로 진행되었으며, 참가자는 조절에 따른 세 가지 조절변인을 모두 경험한 후 각각에 대하여 사용성 평가와 인터뷰를 진행하였다. 실험 결과, 조절은 모든 경험...