A5052736910- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant Gene Expression Analysis
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Plant Physiology and Cultivation Studies
- Plant tissue culture and regeneration
- Plant Genetic and Mutation Studies
- Epigenetics and DNA Methylation
- Chromosomal and Genetic Variations
- Legume Nitrogen Fixing Symbiosis
- Seed Germination and Physiology
- Plant Stress Responses and Tolerance
- Soybean genetics and cultivation
- Flowering Plant Growth and Cultivation
- Plant responses to water stress
- Polysaccharides and Plant Cell Walls
- RNA modifications and cancer
- Plant Disease Resistance and Genetics
- Advanced biosensing and bioanalysis techniques
- Plant-Microbe Interactions and Immunity
- Pluripotent Stem Cells Research
- Neuroethics, Human Enhancement, Biomedical Innovations
- Polyamine Metabolism and Applications
- Genetic Syndromes and Imprinting
University of Edinburgh
2014-2024
Michigan Department of Health and Human Services
2023
University of Michigan
2023
Wellcome Centre for Cell Biology
2016
McMaster University
2016
Technische Universität Berlin
2016
Institute of Plant Molecular Biology
2013
Laboratoire Reproduction et Développement des Plantes
2013
École Normale Supérieure de Lyon
2013
Edinburgh College
2011
Trimethylation of histone H3 lysine 27 (H3K27me3) plays critical roles in regulating animal development, and several cases, H3K27me3 is also required for the proper expression developmentally important genes plants. However, extent to which regulates plant on a genome-wide scale remains unknown. In addition, it not clear whether establishment spreading occur through same mechanisms plants animals. We identified regions containing genome flowering Arabidopsis thaliana using high-density...
In Arabidopsis, the EMBYRONIC FLOWER2 (EMF2), VERNALISATION2 (VRN2) and FERTILISATION INDEPENDENT ENDOSPERM2 (FIS2) genes encode related Polycomb-group (Pc-G)proteins. Their homologues in animals act together with other Pc-G proteins as part of a multimeric complex, Polycomb Repressive Complex 2 (PRC2), which functions histone methyltransferase. Despite similarities between fis2 mutant phenotype those some plant members, it has remained unclear how FIS2/EMF2/VRN2 class interact others. We...
Polycomb repressive complex 2 (PRC2) is a key regulator of epigenetic states catalyzing histone H3 lysine 27 trimethylation (H3K27me3), chromatin mark. PRC2 composition conserved from humans to plants, but the function during early stage plant life unclear beyond fact that it required for development endosperm, nutritive tissue supports embryo growth. Circumventing requirement in endosperm allowed us generate viable homozygous null mutants FERTILIZATION INDEPENDENT ENDOSPERM (FIE), which...
Floral stem cells produce a defined number of floral organs before ceasing to be maintained as cells. Therefore, offer an ideal model study the temporal control cell maintenance within developmental context. AGAMOUS (AG), MADS domain transcription factor essential for termination fate, has long been thought repress gene WUSCHEL (WUS) indirectly. Here, we uncover role Polycomb Group (PcG) genes in temporally precise repression WUS expression and fate. We show that AG directly represses by...
The unusual floral organs (ufo) mutant of Arabidopsis has flowers with variable homeotic organ transformations and inflorescence-like characteristics. To determine the relationship between UFO previously characterized meristem identity genes, we cloned determined its expression pattern. gene shows extensive homology FIMBRIATA (FIM), a mediating genes in Antirrhinum. All three alleles that sequenced are predicted to produce truncated proteins. transcripts were first detected early meristems,...
Summary Leaf shape is determined by polar cell expansion and proliferation along the leaf axes. However, genes controlling during morphogenesis are largely unknown. We identified a dominant mutant of Arabidopsis thaliana , rotundifolia4‐1D (rot4‐1D ), which possessed short leaves floral organs. showed that altered caused reduced proliferation, specifically in longitudinal (proximal–distal) axis leaf, suggesting ROT4 gene controls lateral The open‐reading frame (ORF) encodes novel small...
During Arabidopsis seed development, the growing embryo invades and consumes surrounding endosperm tissue. The signalling pathways that coordinate separation of from concomitant breakdown are poorly understood. We have identified a novel bHLH transcription factor, ZHOUPI (ZOU), which mediates these processes. ZOU is expressed exclusively in developing seeds. It activated central cell immediately after fertilization initially uniformly endosperm, subsequently resolving to region (ESR)....
Seed production in angiosperms requires tight coordination of the development embryo and endosperm. The endosperm-specific transcription factor ZHOUPI has previously been shown to play a key role this process, by regulating both endosperm breakdown formation embryonic cuticle. To what extent these processes are functionally linked is, however, unclear. In order address issue we have concentrated on subtilisin-like serine protease encoding gene ABNORMAL LEAF-SHAPE1. Expression LEAF-SHAPE1 is...
In Arabidopsis, mutations in the Pc-G gene CURLY LEAF (CLF) give early flowering plants with curled leaves. This phenotype is caused by mis-expression of floral homeotic AGAMOUS (AG) leaves, so that ag largely suppress clf phenotype. Here, we identify three despite maintaining high AG expression. We show suppressors correspond to FPA and FT, two genes promoting flowering, SEPALLATA3 (SEP3) which encodes a co-factor for protein. The suppression correlated low SEP3 expression all case reveals...
The Polycomb group (PcG) and trithorax (trxG) genes play crucial roles in development by regulating expression of homeotic other controlling cell fate. Both groups catalyse modifications chromatin, particularly histone methylation, leading to epigenetic changes that affect gene activity. trxG antagonizes the function PcG activating target genes, consequently mutants suppress mutant phenotypes. We previously identified ANTAGONIST OF LIKE HETEROCHROMATIN PROTEIN1 (ALP1) as a genetic suppressor...
Abstract The Polycomb Group (PcG) pathway represses transcription through a mechanism conserved among plants and animals. PcG-mediated repression can determine spatial territories of gene expression, but it remains unclear whether is regulatory requirement for all targets. Here, we show the role PcG proteins in regulation FLOWERING LOCUS T (FT), main activator flowering Arabidopsis thaliana exclusively expressed vasculature. Strikingly, loss causes down-regulation FT. In addition, our...
Summary Arabidopsis guard cell ( GC ) fate is conferred via a transient pulse of expression FAMA that encodes bHLH transcription factor. Stomata often function for years, suggesting the window stabilizes long‐term identity or additional factors operate. Transgenic lines harboring copy transgene were found to induce resetting mature s lineage‐specific stem cells causing new stomata arise within shells old, Stoma‐in‐Stoma SIS phenotype. These disrupt normal trimethylation on lysine 27 histone3...
Seed development in angiosperms demands the tightly co-ordinated of three genetically distinct structures. The embryo is surrounded by endosperm, which turn enclosed within maternally derived seed coat. In Arabidopsis final size determined early expansion coenocytic then cellularizes and subsequently undergoes developmental Programmed Cell Death, breaking down as grows. Endosperm breakdown requires endosperm-specific basic Helix Loop transcription factor ZHOUPI. However, to date mechanism...