A5091473274- Algal biology and biofuel production
- Photosynthetic Processes and Mechanisms
- Microbial Metabolic Engineering and Bioproduction
- Aquatic Ecosystems and Phytoplankton Dynamics
- 3D Printing in Biomedical Research
- Enzyme Catalysis and Immobilization
- Water Quality Monitoring and Analysis
- Biofuel production and bioconversion
- Additive Manufacturing and 3D Printing Technologies
- Protist diversity and phylogeny
- Ultrasound and Cavitation Phenomena
- CRISPR and Genetic Engineering
- Carbon Dioxide Capture Technologies
- Microbial Metabolism and Applications
- Catalytic Processes in Materials Science
- Plasma Applications and Diagnostics
- Metabolomics and Mass Spectrometry Studies
- Biochemical and biochemical processes
- Nerve injury and regeneration
- Marine and coastal ecosystems
- Biodiesel Production and Applications
- Phytochemical Studies and Bioactivities
- Innovative Microfluidic and Catalytic Techniques Innovation
- Electrocatalysts for Energy Conversion
- Nanofabrication and Lithography Techniques
Hongik University
2024-2025
University of Illinois Urbana-Champaign
2020-2024
Bridge University
2024
Chapman University
2024
University of Cambridge
2024
Advanced Biomass R&D Center (South Korea)
2018-2021
Korea Advanced Institute of Science and Technology
2015-2021
Daejeon University
2017
Amorepacific (South Korea)
2011
Abstract Genome editing is crucial for genetic engineering of organisms improved traits, particularly in microalgae due to the urgent necessity next generation biofuel production. The most advanced CRISPR/Cas9 system simple, efficient and accurate some organisms; however, it has proven extremely difficult including model alga Chlamydomonas . We solved this problem by delivering Cas9 ribonucleoproteins (RNPs) comprising protein sgRNAs avoid cytotoxicity off-targeting associated with...
Microalgae are considered promising alternative energy sources because they consume CO2 and accumulate large amounts of lipids that can be used as biofuel. Nannochloropsis is a particularly microalga due to its high growth rate lipid content, the availability genomic information. Transcription factors (TFs) global regulators biological pathways by up- or down-regulation related genes. Among these, basic helix-loop-helix (bHLH) TFs regulate growth, development, stress responses in plants...
Microalgae are considered as excellent platforms for biomaterial production that can replace conventional fossil fuel-based fuels and chemicals. Genetic engineering of microalgae is prerequisite to maximize materials reduce costs the production. Transcription factors (TFs) emerging key regulators metabolic pathways enhance molecules biofuels other materials. TFs with basic leucine zipper (bZIP) domain have been known stress associated lipid metabolism in plants. We overexpressed a bZIP TF,...
Nature uses fibrous structures for sensing and structural functions as observed in hairs, whiskers, stereocilia, spider silks, hagfish slime thread skeins. Here, we demonstrate multi-nozzle printing of 3D hair arrays having freeform trajectories at a very high rate, with fiber diameters fine 1.5 µm, continuous lengths reaching tens centimeters, wide range materials elastic moduli from 5 MPa to 3500 MPa. This is achieved via by rapid solvent exchange yield stress micro granular gel, leading...
Algal biofuel feedstocks are excellent candidates for sustainable and eco-friendly fuels the next generation, which can be improved by genetic modifications their maximal production of biomaterials. However, currently available involve introduction foreign DNA into algal genome, this may face legal public conflicts due to risk environmental, economic, and/or health problems. In regard, we employed an old concept crop improvement that has been accepted in long history agriculture, i.e....
Chlorophylls play important roles in photosynthesis, and thus are critical for growth related metabolic pathways photosynthetic organisms. They particularly microalgae, emerging as the next generation feedstock biomass biofuels. Nannochloropsis industrial microalgae these purposes, but peculiar that they lack accessory chlorophylls. In addition, localization of heterologous proteins to chloroplast has not been fully studied, due secondary plastid surrounded by four membranes. This study...
Microalgae's low tolerance to high CO
Microalgal strain development through genetic engineering has received much attention as a way to improve the traits of microalgae suitable for biofuel production. However, there are still some limitations in application genetically modified organisms. In this regard, been recent interest isolation and characterization superior strains naturally and/or adapted under certain condition on interpretation phenotypic changes whole genome sequencing. study, we isolated characterized novel...
Microalgae, valued for their sustainability and CO2 fixation capabilities, are emerging as promising sources of biofuels high-value compounds. This study aimed to boost lipid production in C. reinhardtii by overexpressing chloroplast glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a key enzyme the Calvin cycle glycolysis, under control nitrogen-inducible NIT1 promoter, positively impact overall carbon metabolism. The standout transformant, PNG#7, exhibited significantly increased nitrogen...
Most cosmetic and therapeutic applications of Clostridium botulinum neurotoxin (BoNT) are related to muscle paralysis caused by the blocking neurotransmitter release at neuromuscular junction. BoNT specifically cleaves SNARE proteins nerve terminal impairs neuroexocytosis. Recently, we have shown that several polyphenols inhibit from neuronal PC12 cells interfering with complex formation. Based on our previous result, report here myricetin, delphinidin, cyanidin indeed paralyze inhibiting...
Fructophilic behavior in microalgae is a rare trait that could benefit biorefineries by enabling substitution of carbon source with fructose, and our previous study identified Ettlia sp. prefers fructose relative to glucose. In this study, analyzing the transcription levels genes related sugar transport glycolysis pathway, utilization was investigated. fructose-containing medium, expression fructokinase (EttFRK3) glucokinase (EttGCK1 EttGCK2) were significantly upregulated heterotrophic...
A small and efficient DNA mutation-inducing machine was constructed with an array of microplasma jet devices (7 × 1) that can be operated at atmospheric pressure for microbial mutagenesis. Using this machine, we report disruption a plasmid generation mutants oleaginous yeast Rhodosporidium toruloides. Specifically, compact-sized channel (25 20 2 mm3) capable generating electron density greater than 1013 cm-3 to produce reactive species (N2*, N2+, O, OH, Hα) under helium conditions induce The...