A5031417330- Extracellular vesicles in disease
- RNA Interference and Gene Delivery
- Cellular transport and secretion
- Immunotherapy and Immune Responses
- Lipid Membrane Structure and Behavior
- MicroRNA in disease regulation
- Phagocytosis and Immune Regulation
- Advanced biosensing and bioanalysis techniques
- Immune cells in cancer
- Nanoplatforms for cancer theranostics
- Cancer Immunotherapy and Biomarkers
- Neuroscience and Neuropharmacology Research
- Biotin and Related Studies
- Wound Healing and Treatments
- CAR-T cell therapy research
- Immune Cell Function and Interaction
- Nanoparticle-Based Drug Delivery
- Tissue Engineering and Regenerative Medicine
- Calcium signaling and nucleotide metabolism
- Biochemical and Structural Characterization
- RNA regulation and disease
- Drug Transport and Resistance Mechanisms
- Viral Infections and Vectors
- Neurological disorders and treatments
- Mast cells and histamine
Korea Institute of Science and Technology
2016-2025
Korea University of Science and Technology
2017-2025
Bio-Medical Science (South Korea)
2017-2025
Sungkyunkwan University
2008-2016
Korean Association Of Science and Technology Studies
2014
Stanford University
2007
Macrophages (Mϕs) critically contribute to wound healing by coordinating inflammatory, proliferative, and angiogenic processes. A proper switch from proinflammatory M1 anti-inflammatory M2 dominant Mϕs accelerates the processes leading favorable wound-care outcomes. Herein, an exosome-guided cell reprogramming technique is proposed directly convert for effective management. The Mϕ-derived exosomes (M2-Exo) induce a complete conversion of in vitro. reprogrammed turn Arginase (M2-marker) iNOS...
Parkinson disease and dementia with Lewy bodies are featured the formation of composed mostly α-synuclein (α-Syn) in brain. Although evidence indicates that large oligomeric or protofibril forms α-Syn neurotoxic agents, detailed mechanisms toxic functions oligomers remain unclear. Here, we show efficiently inhibit neuronal SNARE-mediated vesicle lipid mixing. Large preferentially bind to N-terminal domain a vesicular SNARE protein, synaptobrevin-2, which blocks mixing by preventing complex...
Abstract Macrophages (Mφs) are characterized by remarkable plasticity, an essential component of chronic inflammation. Thus, appropriate and timely transition from proinflammatory (M1) to anti‐inflammatory (M2) Mφs during wound healing is vital promoting resolution acute inflammation enhancing tissue repair. Herein, exosomes derived M2‐Mφs (M2‐Exos), which contain putative key regulators driving Mφ polarization, used as local microenvironmental cues induce reprogramming M1‐Mφs toward for...
Abstract Lipid nanoparticles (LNPs) exhibit remarkable mRNA delivery efficiency, yet their majority accumulate in the liver or spleen after injection. Tissue‐specific can be achieved through modulating LNP properties, such as tuning PEGylation varying lipid components systematically. In this paper, a streamlined method is used for incorporating tumor‐targeting peptides into LNPs; programmed death ligand 1 (PD‐L1) binding are conjugated to PEGylated lipids via copper‐free click reaction, and...
An efficient system for direct delivery of integral membrane proteins is successfully developed using a new biocompatible exosome-based platform. Fusogenic exosomes harboring viral fusogen, vascular stomatitis virus (VSV)-G protein, can fuse with and modify plasma membranes in process called "membrane editing." This facilitate the transfer biologically active into target cell both vitro vivo.
Abstract Activation of T cell immune response is critical for the therapeutic efficacy cancer immunotherapy. Current immunotherapies have shown remarkable clinical success against several cancers; however, significant responses remain restricted to a minority patients. Here, we show strategy that combines enhancing phagocytic activity antigen-presenting cells with immunogenic death trigger efficient antitumour immunity. Rho-kinase (ROCK) blockade increases phagocytosis and induces immunity...
Boiling histotripsy is a promising non-invasive High-Intensity Focused Ultrasound (HIFU) technique that employs HIFU mechanical effects to fractionate solid tumours without causing any significant thermal damage. It has been suggested boiling may induce strong immune response due the absence of denatured antigenic protein at focus. The underlying immunological mechanisms this are, however, poorly understood. In study, we demonstrated feasibility using mechanically human breast adenocarcinoma...
Human hair dermal papillary (DP) cells comprising mesenchymal stem in follicles contribute critically to growth and cycle regulation. The transition of from telogen anagen phase is the key regulating growth, which relies heavily on activation DP cells. In this paper, we suggested exosomes derived bovine colostrum (milk exosomes, Milk-exo) as a new effective non-surgical therapy for loss. Results showed that Milk-exo promoted proliferation rescued dihydrotestosterone (DHT, androgen...
In this study, we examined the potentially beneficial effects of bovine colostrum-derived exosomes on UV-induced aging and damage in three major resident skin cells including keratinocytes, melanocytes, fibroblasts. The treatment with colostrum prevented generation intracellular reactive oxygen species epidermal keratinocytes. UV-stimulated could also significantly reduce production protective skin-darkening pigment melanin, which may help to risk excessive melanin formation causing...
Despite the rise in global burden of inflammatory bowel disease, there is a lack safe and effective therapies that can meet needs clinical patients. In this study, we investigated beneficial effects bovine milk, especially colostrum-derived exosomes (Col-exo) murine model ulcerative colitis induced by dextran sodium sulfate (DSS). Col-exo activated proliferation colonic epithelial cells macrophages, created an environment to relieve inflammation effectively removing reactive oxygen species...
Abstract While proteolysis‐targeting chimeras (PROTACs) hold great potential for persistently reprogramming the immunosuppressive tumor microenvironment via targeted protein degradation, precisely activating them in tissues and preventing uncontrolled proteolysis at off‐target sites remain challenging. Herein, a light‐triggered PROTAC nanoassembly (LPN) photodynamic indoleamine 2,3‐dioxygenase (IDO) is reported. The LPN derived from self‐assembly of prodrug conjugates, which comprise PROTAC,...
While mesalamine, a 5-aminosalicylic acid (5-ASA), is pivotal in the management of inflammatory bowel disease (IBD) through both step-up and top-down approaches clinical settings, its widespread utilization limited by low bioavailability at desired site action due to rapid extensive absorption upper gastrointestinal (GI) tract. Addressing mesalamine's pharmacokinetic challenges, here, we introduce nanoassemblies composed exclusively mesalamine prodrug that pairs 5-ASA with mucoadhesive...
In neurons, synaptotagmin 1 (Syt1) is thought to mediate the fusion of synaptic vesicles with plasma membrane when presynaptic Ca2+ levels rise. However, in vitro reconstitution experiments have failed recapitulate key characteristics Ca2+-triggered fusion. Using an single-vesicle assay, we found that membrane-anchored Syt1 enhanced sensitivity and speed. This stimulatory activity dropped as level rose beyond physiological levels. Thus, requires anchor stimulate vesicle at may function a...
Alzheimer's disease (AD) is closely associated with synaptic dysfunction, and thus current treatments often aim to stimulate neurotransmission improve cognitive impairment. Whereas the formation of soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex essential for transmission, correlation between SNAREs AD neuropathology unknown. Here, we report that intracellular amyloid-β (Aβ) oligomers directly inhibit SNARE-mediated exocytosis by impairing SNARE...
Abstract A growing appreciation of the relationship between immune system and tumorigenesis has led to development strategies aimed at “re‐editing” kill tumors. Here, a novel tactic is reported for overcoming activation‐energy threshold immunosuppressive tumor microenvironment mediating delivery presentation neoantigens host's system. This nature‐derived nanocage not only efficiently presents ligands that enhance cancer cell phagocytosis, but also delivers drugs induce immunogenic death. The...
Adv. Funct. Mater. 2018, 28, 1703074 The acknowledgements as initially published for this manuscript are missing a funding statement. corrected should read follows: This work was supported by grant from the National R&D Program Cancer Control, Ministry of Health and Welfare, Republic Korea (1420390), Research Foundation (NRF) funded government (2017R1A2B2010292 2017R1A3B1023418), KU-KIST Graduate School Converging Science Technology Program, KIST Institutional Program. authors apologize any...
As wound healing is an extremely complicated process, consisting of a cascade interlocking biological events, successful requires multifaceted approach to support appropriate and rapid transitions from the inflammatory proliferative remodeling phases. In this regard, here potential use bovine milk extracellular vesicles (EVs) enhance investigated. The results show that EVs promote fibroblast proliferation, migration, endothelial tube formation. particular, derived colostrum (Colos EVs)...