Boiling histotripsy is a promising non-invasive High-Intensity Focused Ultrasound (HIFU) technique that employs HIFU mechanical effects to fractionate solid tumours without causing any significant thermal damage. It has been suggested boiling may induce strong immune response due the absence of denatured antigenic protein at focus. The underlying immunological mechanisms this are, however, poorly understood. In study, we demonstrated feasibility using mechanically human breast adenocarcinoma...

ABSTRACT As emerging therapeutic strategies for aging and age‐associated diseases, various biochemical approaches have been developed to selectively remove senescent cells, but how physical stimulus influences cells its possible application in senolytic therapy has not reported yet. Here we a method stimulate via low‐intensity pulsed ultrasound (LIPUS) treatment. LIPUS stimulation did affect the cell cycle, enhanced secretion of specific cytokines known as senescence‐associated secretory...

Background. Stroke affects widespread brain regions through interhemispheric connections by influencing bilateral motor activity. Several noninvasive stimulation techniques have proved their capacity to compensate the functional loss manipulating neural activity of alternative pathways. Over past few decades, therapies been tailored within theoretical framework modulation cortical excitability enhance adaptive plasticity after stroke. Objective. However, considering vast difference between...

Boiling histotripsy is a High Intensity Focused Ultrasound (HIFU) technique which uses number of short pulses with high acoustic pressures at the HIFU focus to induce mechanical tissue fractionation. In boiling histotripsy, two different types cavitation contribute towards destruction: vapour bubble and clouds. An understanding mechanisms underpinning these phenomena their dynamics therefore paramount predicting controlling overall size lesion produced for given exposure condition. A studies...

A phenomenological implementation of Classical Nucleation Theory (CNT) is employed to investigate the connection between high intensity focused ultrasound (HIFU) pressure and temperature fields with energetic requirements bubble nucleation. As a case study, boiling histotripsy in tissue-mimicking phantoms modelled. The physics key components CNT HIFU conditions such as derivation nucleation thresholds approximations regarding surface tension liquid are reviewed discussed. Simulations show...

This paper interrogates the intersections between bubble dynamics and classical nucleation theory (CNT) towards constructing a model that describes intermediary events extrema of cavitation boiling. We employ Zeldovich's hydrodynamic approach to obtain description nuclei grow simultaneously via excitation by acoustic field vapour transport. By quantifying relative dominance both mechanisms, it is then possible discern extent which viscosity, inertia, surface tension transport shape growth...

Obesity is known to increase the risk and severity of age-related macular degeneration (AMD). Increased inflamed metabolic activity visceral adipose tissue (VAT) considered as a crucial underlying mechanism for harmful effects obesity. In this study, we aimed investigate VAT with

Abstract Boiling histotripsy (BH) is a completely non‐invasive ultrasonic technique that can be used to mechanically destroy tumor tissues. Studies have shown BH has biological effects on immune responses, but the mechanisms involved in induction and enhancement of systemic anti‐tumor responses after treatment are poorly understood. The present study therefore investigates triggered by exposure vivo. In syngeneic model, results more dendritic cell maturation increased intra‐tumoral...

Abstract A blood–brain barrier (BBB) opening induced by focused ultrasound (FUS) has been widely studied as an effective way of treating brain diseases. We investigate the effect ultrasound’s incidence angle at caudate putamen ( Cp ) and thalamus Th rat inducing same power that corresponds to acoustic pressure 0.65 MPa in free field. The BBB permeability (K trans was quantitatively evaluated with dynamic contrast-enhanced magnetic resonance imaging. group averaged (n = 11) maximum K (0.021 ±...

Hepatocyte transplantation has been considered and investigated as a promising alternative method to liver for treating liver-based metabolic disorder in newborns over the past two decades. Although some clinical trials have conducted shown benefits outcomes, it is difficult deliver achieve desired level of integration hepatocytes parenchyma. To overcome this problem, work introduces an portal-infused-hepatocyte cell transplantation. improve engraftment transplantable hepatocytes, these are...

Abstract Liver fibrosis is a hallmark of chronic liver disease which could lead to cirrhosis or cancer. However, there currently lack direct treatment for fibrosis. Boiling histotripsy (BH) an emerging non-invasive high-intensity focused ultrasound technique that can be employed mechanically destruct solid tumour at the focus via acoustic cavitation without significant adverse effect on surrounding tissue. Here, we investigated whether BH fractionate fibrotic tissue thereby exhibiting...

In the present study, a novel hybrid method was considered to identify and measure inertial cavitation activity using acoustic optical emissions from violent bubble collapses. A photomultiplier (PMT) tube calibrated cylindrical needle hydrophone were used simultaneously detect sonochemical luminescence (SCL) signals emissions, respectively, during sonication. focusing ultrasound transducer operating at 398.4 kHz employed produce dense cloud focus. The results clearly showed that similar...

Boiling histotripsy is a promising High-Intensity Focused Ultrasound (HIFU) technique that can be used to induce mechanical tissue fractionation at the HIFU focus via cavitation. Two different types of cavitation produced during boiling exposure contribute towards destruction: (1) vapour bubble and (2) clouds in between source. Control extent degree damage by necessary when treating solid tumour adjacent normal or major blood vessels. This is, however, difficult achieve with due stochastic...

Introduction Boiling histotripsy (BH) is a promising High Intensity Focused Ultrasound (HIFU) technique that can be used to mechanically fractionate solid tumours at the HIFU focus noninvasively, promoting tumour immunity. Because of occurrence shock scattering phenomenon during BH process, treatment accuracy is, however, somewhat limited. To induce more localised and selective tissue destruction, concept pressure modulation has recently been proposed in our previous vitro phantom study. The...

Abstract Boiling histotripsy (BH) is a promising High-Intensity Focused Ultrasound technique that can be employed to mechanically fractionate solid tumours. Whilst studies have shown the feasibility of BH destroy liver cancer, no study has reported on healing process BH-treated tissue. We therefore extensively investigated evolution response in order provide an insight into mechanisms. In present study, 14 Sprague Dawley rats underwent treatment and were sacrificed days 0, 3, 7, 14, 28 for...

Whilst a number of studies have demonstrated that low-intensity pulsed ultrasound (LIPUS) is promising therapeutic technique can be used for delivering mild mechanical stimuli to target tissue non-invasively, the underlying biophysical mechanisms still remain unclear. Most mechanism focused explicitly on effects LIPUS cell membrane and mechanosensitive receptors. In present study, we propose an additional by which propagation through living cells may directly impact intracellular dynamics,...

This work aims to establish a theoretical framework for the modeling of bubble nucleation in histotripsy. A phenomenological version classical theory was parametrized with histotripsy experimental data, fitting temperature-dependent activity factor that harmonizes predictions and data at both high low temperatures. Simulations pressure temperature fields are then used order understand spatial temporal properties varying sonication conditions. offers thermodynamic understanding on role...

Whilst sonothrombolysis is a promising and noninvasive ultrasound technique for treating blood clots, bleeding caused by thrombolytic agents used dissolving clots potential obstruction of flow detached (i.e., embolus) are the major limitations current approach. In present study, new method proposed embolus without use drugs. Our involves (a) generating spatially localised acoustic radiation force in vessel against to trap moving generation an net), (b) producing cavitation mechanically...