A5082021838- Monoclonal and Polyclonal Antibodies Research
- Advanced Fluorescence Microscopy Techniques
- HER2/EGFR in Cancer Research
- Protein Degradation and Inhibitors
- Chronic Lymphocytic Leukemia Research
- Cancer Treatment and Pharmacology
- Biotin and Related Studies
- Bioinformatics and Genomic Networks
- Cell death mechanisms and regulation
- Microtubule and mitosis dynamics
- Advanced Biosensing Techniques and Applications
- Antenna Design and Analysis
- 14-3-3 protein interactions
- Cellular transport and secretion
- Agriculture, Soil, Plant Science
- Smart Grid and Power Systems
- Antenna Design and Optimization
- ATP Synthase and ATPases Research
- Lipid Membrane Structure and Behavior
- Multiple Myeloma Research and Treatments
- Artificial Intelligence in Games
- Fungal Biology and Applications
- Video Analysis and Summarization
- Speech and Audio Processing
- Enzyme Structure and Function
Chungnam National University
2023
Chungnam National University Hospital
2023
Seoul National University
2018
Korea Advanced Institute of Science and Technology
2010-2015
Eulji University
2014
Korea Research Institute of Bioscience and Biotechnology
2014
Soongsil University
2014
Inha University
2006
In neurons, synaptotagmin 1 (Syt1) is thought to mediate the fusion of synaptic vesicles with plasma membrane when presynaptic Ca2+ levels rise. However, in vitro reconstitution experiments have failed recapitulate key characteristics Ca2+-triggered fusion. Using an single-vesicle assay, we found that membrane-anchored Syt1 enhanced sensitivity and speed. This stimulatory activity dropped as level rose beyond physiological levels. Thus, requires anchor stimulate vesicle at may function a...
Co-immunoprecipitation (co-IP) has become a standard technique, but its protein-band output provides only static, qualitative information about protein-protein interactions. Here we demonstrate real-time single-molecule co-IP technique that generates videos of individual interactions as they occur in unpurified cell extracts. By analysing single Ras-Raf with 50-ms time resolution, have observed transient intermediates the interaction and determined all essential kinetic rates. Using this...
Abstract Introduction: Pharmacological inhibition of anti-apoptotic proteins, especially with BCL2 inhibitors (i), has proven its clinical efficacy for treating patients hematological cancers. However, there are not yet widely available tools to predict the sensitivity individual different drugs targeting BCL2, MCL1, or BCLXL. A new technology, Single-molecule Protein Interaction Detection (SPID), based on immunoprecipitation proteins followed by addition fluorescently labeled probes and...
Abstract Introduction: Acute myeloid leukemia (AML) is a genetically heterogeneous malignancy, leading to varied therapeutic outcomes with targeted therapies. Venetoclax, BCL2 inhibitor, has shown efficacy in treating AML patients by disrupting functions. Genomic mutations like TP53, IDH1/2, PTPN11, and NRAS are associated response rates venetoclax. However, profiling the association between these BCL2-family protein interactions remains limited clinical specimens. This study utilized...
Abstract The BCL2 protein family controls apoptosis by shifting the dynamic equilibrium state of complex formation. BH3 mimetics disrupt this balance disturbing interaction a specific anti-apoptotic protein, leading to initiation apoptosis. However, relying solely on molecular data for can be challenging due functional redundancy within and their network. Therefore, comprehensive profiling information considering complexes is essential determine apoptotic dependencies, particularly in...
Abstract B-cell-lymphoma-2 (BCL2) homology-3 (BH3) mimetics are inhibitors of protein–protein interactions (PPIs) that saturate anti-apoptotic proteins in the BCL2 family to induce apoptosis cancer cells. Despite success BH3-mimetic ABT-199 for treatment haematological malignancies, only a fraction patients respond drug and most eventually develop resistance it. Here we show efficacy can be predicted by profiling rewired status PPI network via single-molecule pull-down co-immunoprecipitation...
Abstract BH3 mimetics are protein-protein interaction (PPI) inhibitors that saturate anti-apoptotic proteins in the BCL2 family to induce apoptosis cancer cells, a prominent example of protein complex-targeting therapies. Despite remarkable success mimetic ABT-199 treating hematological malignancies, only fraction patients respond and eventually develop resistance, necessitating predictive biomarkers for both initial responses resistance development. We here used single-molecule pull-down...
Although the research for computer-based Go AI engines started at about 1980's, is currently considered as last board game which need to be implemented using computer systems. Most current implementations are tree-search technique find best moves, however it nearly impossible review all possible candidate moves in games. In this paper, we propose a totally different approach: data-driven engine. Hugh amount of log database stored Hadoop servers support parallel pattern retrieval. each...
The conventional co-immunoprecipitation provides static and qualitative information about protein-protein interactions. Lee et al. report real-time imaging of process with single-molecule resolution, allowing for characterization the native Ras proteins derived from individual cancers.
Topic: 13. Myeloma and other monoclonal gammopathies - Biology & Translational Research Background: The protein-protein interactions (PPIs) between members of the BCL2 family is underlying mechanism for regulation apoptosis pathway. In various types cancers, cancer cells evade by upregulating anti-apoptotic proteins like BCL2. development PPI inhibitors has enabled selective disruption target proteins’ PPIs, leading to initiation signaling in cancers. As a result, an assessment changes...
Micrococcus luteus (M. luteus)-induced endophthalmitis is very rare and may present as either acute or chronic postoperative endophthalmitis. The aim of this study was to report a case delayed-onset M. luteus-induced that occurred several months after cataract surgery.A 78-year-old man presented with decreased vision, pain redness in the right eye had begun 3 days prior. He undergone surgery 4 mo Visual acuity counting fingers; slit-lamp examination revealed conjunctival injection, posterior...