A5060356548- Hippo pathway signaling and YAP/TAZ
- CRISPR and Genetic Engineering
- Cancer Genomics and Diagnostics
- Wnt/β-catenin signaling in development and cancer
- Pluripotent Stem Cells Research
- Advanced biosensing and bioanalysis techniques
- Monoclonal and Polyclonal Antibodies Research
- Cancer Cells and Metastasis
- Melanoma and MAPK Pathways
- Advanced Fluorescence Microscopy Techniques
- Pancreatic and Hepatic Oncology Research
- Gene Regulatory Network Analysis
- Ubiquitin and proteasome pathways
- Protein Degradation and Inhibitors
- Pancreatic function and diabetes
- PI3K/AKT/mTOR signaling in cancer
- 14-3-3 protein interactions
- Cancer-related gene regulation
- Advanced Biosensing Techniques and Applications
- Hematopoietic Stem Cell Transplantation
- Neonatal Respiratory Health Research
- Zebrafish Biomedical Research Applications
- Heat shock proteins research
- Histone Deacetylase Inhibitors Research
- Cell death mechanisms and regulation
Korea University
2023-2025
Korea Institute of Science and Technology
2020-2025
Bio-Medical Science (South Korea)
2023-2025
Massachusetts Institute of Technology
2019-2025
Korea University of Science and Technology
2022-2025
Korea Advanced Institute of Science and Technology
2009-2017
Creative Research
2013
Initiatives (Denmark)
2013
The switch between stem/progenitor cell expansion and differentiation is critical for organ homeostasis. mammalian Hippo pathway effector oncoprotein YAP expands undifferentiated cells in various tissues. However, the YAP-associated transcription factors downstream targets underlying this stemness-promoting activity are poorly understood. Here we show that SRF-IL6 axis mediator of YAP-induced stemness mammary epithelial breast cancer. Specifically, serum response factor (SRF)-mediated...
Mammalian sterile 20-like kinases 1 and 2 (Mst1 Mst2, respectively) are potent serine/threonine that involved in cell proliferation death. To investigate the physiological functions of Mst1 we generated Mst2 mutant mice. Mst1(-/-) Mst2(-/-) mice were viable fertile developed normally, suggesting possible functional overlaps between two genes. A characterization heterozygous homozygous combinations showed containing a single copy either gene underwent normal organ development; however,...
Co-immunoprecipitation (co-IP) has become a standard technique, but its protein-band output provides only static, qualitative information about protein-protein interactions. Here we demonstrate real-time single-molecule co-IP technique that generates videos of individual interactions as they occur in unpurified cell extracts. By analysing single Ras-Raf with 50-ms time resolution, have observed transient intermediates the interaction and determined all essential kinetic rates. Using this...
Respiratory distress syndrome (RDS), which is induced by insufficient production of surfactant, the leading cause mortality in preterm babies. Although several transcription factors are known to be involved surfactant protein expression, molecular mechanisms and signaling pathways upstream these have remained elusive. Here, using mammalian Hippo kinases (Mst1/2, sterile 20-like kinase 1/2) conditional knockout mice, we demonstrate that Mst1/2 critical for orchestration homeostasis prevention...
Tyrosine kinases play a crucial role in cell proliferation and survival are extensively investigated as targets for cancer treatment. However, the efficacy of most tyrosine kinase inhibitors (TKIs) therapy is limited due to resistance. In this study, we identify synergistic combination involving TKIs treatment triple negative breast cancer. By employing pairwise knockout CRISPR screens, FYN KDM4 critical whose inhibition enhances effectiveness TKIs, such NVP-ADW742 (IGF-1R inhibitor),...
Human pancreatic ductal adenocarcinoma (PDAC) is a cancer with dismal prognosis. The efficacy of PDAC anticancer therapies often short-lived; however, there little information on how this disease entity so frequently gains resistance to treatment. We adopted the concept stem cells (CSCs) explain mechanism and evaluated candidate drug target these therapy-resistant CSCs. identified subpopulation in CSC features that were enriched for aldehyde dehydrogenase (ALDH), marker expressed certain...
// Gun-Soo Park 1 , Hyangyee Oh 2 Minchul Kim Tackhoon Randy L. Johnson 3 Kenneth D. Irvine Dae-Sik Lim National Creative Research Center for Cell Division and Differentiation, Department of Biological Sciences, Korea Advanced Institute Science Technology (KAIST), Daejeon, South Howard Hughes Medical Institute, Waksman Molecular Biology Biochemistry, Rutgers University, Brunswick, New Jersey, USA Cancer Biology, University Texas, M.D. Anderson Center, Houston, Correspondence to: Lim, e-mail:...
Abstract Background BRAF inhibitors are widely employed in the treatment of melanoma with V600E mutation. However, development resistance compromises their therapeutic efficacy. Diverse genomic and transcriptomic alterations found inhibitor resistant melanoma, posing a pressing need for convergent, druggable target that reverse therapy tumor different mechanisms. Methods CRISPR-Cas9 screens were performed to identify novel gene whose inhibition selectively targets A375VR, mutant cell line...
The Ets transcription factor Er71 is an important regulator of endothelial and hematopoietic development during mammalian embryogenesis. However, the role in adult hematopoiesis has remained unknown. We now first show that conditional deletion system mice results a marked reduction (55%) number stem cells (HSCs) likely due to increased cell death. Bone marrow transplantation (BMT) experiments further confirmed required for repopulation HSCs. In addition, Er71(+/-) exhibited slight decrease...
Abstract Temporal modulation of the expression multiple genes underlies complex biological phenomena. However, there are few scalable and generalizable gene circuit architectures for programming sequential genetic perturbations. Here, we describe a modular recombinase-based architecture, comprising tandem perturbation cassettes (GPCs), that enables in defined temporal order by alternating treatment with just two orthogonal ligands. We use GPCs to sequentially express single-guide RNAs encode...
Fine regulation of differentiation and expansion adult stem cells is required for the maintenance tissue homeostasis. Deregulation toward a common characteristic poorly differentiated cancer cells. In this context, YAP (Yes associated protein) has gained considerable research attention as critical mediator involved in promoting inhibiting often highly expressed stem/progenitor compartments its overexpression leads to uncontrolled various epithelial tissues, such lung, skin, intestine.1...
Abstract Background CRISPR-based screens are revolutionizing drug discovery as tools to identify genes whose ablation induces a phenotype of interest. For instance, CRISPR-Cas9 screening has been successfully used novel therapeutic targets in cancer where disruption leads decreased viability malignant cells. However, low-activity guide RNAs may give rise variable changes phenotype, preventing easy identification hits and leading false negative results. Therefore, correcting the effects bias...
Tyrosine kinases play a crucial role in cell proliferation and survival are extensively investigated as targets for cancer treatment. However, the efficacy of most tyrosine kinase inhibitors (TKIs) therapy is limited due to resistance. In this study, we identify synergistic combination involving TKIs treatment triple negative breast cancer. By employing massively parallel combinatorial CRISPR screens, FYN KDM4 critical whose inhibition enhances effectiveness TKIs, such NVP-ADW742 (IGF-1R...