A5003764510- Neurobiology and Insect Physiology Research
- Invertebrate Immune Response Mechanisms
- Insect and Arachnid Ecology and Behavior
- Alzheimer's disease research and treatments
- Physiological and biochemical adaptations
- Insect Utilization and Effects
- Monoclonal and Polyclonal Antibodies Research
- Trace Elements in Health
- MicroRNA in disease regulation
- Drug Transport and Resistance Mechanisms
- Plant biochemistry and biosynthesis
- Plant Molecular Biology Research
- Insect and Pesticide Research
- Photochromic and Fluorescence Chemistry
- Animal Behavior and Reproduction
- Cell Image Analysis Techniques
- Molecular spectroscopy and chirality
- Insect Pest Control Strategies
- Eicosanoids and Hypertension Pharmacology
- Receptor Mechanisms and Signaling
- Protein Structure and Dynamics
- Heat shock proteins research
- Silk-based biomaterials and applications
- Insect Resistance and Genetics
- HER2/EGFR in Cancer Research
Korea Advanced Institute of Science and Technology
2008-2019
Rockefeller University
2004-2006
Edith Cowan University
2003
Case Western Reserve University
2003
Massachusetts General Hospital
2001
Harvard University
2001
Abstract: Cu and Zn have been shown to accumulate in the brains of Alzheimer's disease patients. We previously reported that 2+ bind amyloid β (Aβ), explaining their enrichment plaque pathology. Here we detail stoichiometries binding affinities multiple cooperative ‐binding sites on synthetic Aβ1‐40 Aβ1‐42. developed a ligand displacement technique (competitive metal capture analysis) uses metal‐chelator complexes evaluate ion Aβ, notoriously self‐aggregating peptide. This analysis indicated...
We have previously reported that amyloid Aβ, the major component of senile plaques in Alzheimer's disease (AD), binds Cu with high affinity via histidine and tyrosine residues [Atwood, C. S., et al. (1998) J. Biol. Chem. 273, 12817−12826; Atwood, (2000) Neurochem. 75, 1219−1233] produces H2O2 by catalyzing reduction Cu(II) or Fe(III) [Huang, X., (1999) Biochemistry 38, 7609−7616; Huang, 274, 37111−37116]. Incubation induces SDS-resistant oligomerization Aβ 1219−1233], a feature...
Co-immunoprecipitation (co-IP) has become a standard technique, but its protein-band output provides only static, qualitative information about protein-protein interactions. Here we demonstrate real-time single-molecule co-IP technique that generates videos of individual interactions as they occur in unpurified cell extracts. By analysing single Ras-Raf with 50-ms time resolution, have observed transient intermediates the interaction and determined all essential kinetic rates. Using this...
Abstract MicroRNAs (miRNAs) regulate many physiological processes including body growth. Insulin/IGF signalling is the primary regulator of animal growth, but extent to which miRNAs act in insulin-producing cells (IPCs) unclear. Here we generate a UAS-miRNA library Drosophila stocks and perform genetic screen identify whose overexpression IPCs inhibits growth . Through this screen, miR-9a as an evolutionarily conserved insulin IPC-specific reduces size. Of predicted targets , find that loss...
Like most animals, insects rely on their olfactory systems for finding food and mates in avoiding noxious chemicals predators. Most insect neurons express an odorant-specific odorant receptor (OR) along with Orco, the co-receptor. Orco binds ORs permits trafficking to dendrites of antennal sensory (OSNs), where together, they are suggested form heteromeric ligand-gated non-selective cation channels. While amino acid residues well conserved across orders, one especially well-conserved region...
Here, we show that the enzymatic cofactor tetrahydrobiopterin (BH4) inhibits feeding in Drosophila. BH4 biosynthesis requires sequential action of conserved enzymes Punch, Purple, and Sepiapterin Reductase (Sptr). Although observe increased upon loss Punch Purple adult fat body, Sptr must occur brain. We found expression is required four neurons express neuropeptide F (NPF), fly homologue vertebrate appetite regulator Y (NPY). As expected, flies rescues body NPF neurons. Mechanistically,...
Animal olfactory systems detect volatile environmental chemicals and integrate this information to direct the discovery of food mates as well danger avoidance. Rather than remaining constant, response thresholds are modulated by internal external cues adapt odor-guided behaviors changing conditions.Here, we show in Drosophila melanogaster that neuropeptide F (NPF) modulates responses a specific population antennal sensory neurons (OSNs) food-derived odors. We knock-down NPF specifically...
Abstract Mammalian T-type Ca 2+ channels are encoded by three separate genes (Ca v 3.1, 3.2, 3.3). These reported to be sleep stabilizers important in the generation of delta rhythms deep sleep, but controversy remains. The identification precise physiological functions for has been hindered, at least part, potential compensation between products these and a lack specific pharmacological inhibitors. Invertebrates have only one channel gene, its even less well-studied. We cloned Ca-α1T, 3...
Abstract The Drosophila olfactory system is highly stereotyped in form and function; sensory neurons (OSNs) expressing a specific odorant receptor (OR) always appear the same antennal location axons of OSNs OR converge on lobe glomeruli. Although some transcription factors have been implicated combinatorial code specifying expression OSN identity, it clear other players remain unidentified. In hopes mitigating challenges genome-wide screening, we examined feasibility two-tiered approach...
Abstract Olfactory sensing and its modulation are important for the insects in recognizing diverse odors from environment making correct decisions to survive. Identifying new genes involved olfactory unveiling their mechanisms may lead us understand decision processes central nervous system. Here, we report a novel function of cyclic nucleotide‐gated (CNG) channel CG42260 modulating ab3A sensory neurons, which specifically respond food‐derived fruit fly Drosophila melanogaster . We found...
Animals use their odorant receptors to receive chemical information from the environment. Insect differ G protein-coupled in vertebrates and nematodes, very little is known about protein-protein interactions. Here, we introduce a mass spectrometric platform designed for large-scale analysis of insect receptor Using this platform, obtained first Orco interactome
Abstract In a recent paper, Cayirlioglu et al. report that the disruption of specific miRNA, miR‐279 , which normally acts to inhibit transcription factor Nerfin‐1, uncovers population hybrid CO 2 neurons in Drosophila maxillary palp. 1 Normally, fruit fly are found only antennae, while mosquito palps. The this miRNA mutant may, thus, recapitulate an evolutionary intermediate unseen since divergence these two dipteran lineages over 250 million years ago. BioEssays 30:621–623, 2008. © 2008...
The conventional co-immunoprecipitation provides static and qualitative information about protein-protein interactions. Lee et al. report real-time imaging of process with single-molecule resolution, allowing for characterization the native Ras proteins derived from individual cancers.
Summary Large scale genetic screening is tedious and time-consuming. To address this problem, we propose a novel two-tiered system comprising an initial “pooling” screen that identifies miRNAs whose tissue-specific over-expression causes phenotype of interest followed by more focused secondary uses gene-specific RNAi. As inhibit translation or direct the destruction their target mRNAs, any observed with miRNA can be attributed to loss-of-function one mRNAs. Since miRNA-target pairing...