A5080325866- Microbial Metabolic Engineering and Bioproduction
- biodegradable polymer synthesis and properties
- Enzyme Catalysis and Immobilization
- Asymmetric Synthesis and Catalysis
- Biofuel production and bioconversion
- Synthesis of Organic Compounds
- Chemical Synthesis and Analysis
- Chemical synthesis and alkaloids
- GABA and Rice Research
- Cancer Research and Treatments
- Oxidative Organic Chemistry Reactions
- Biochemical and biochemical processes
- Microplastics and Plastic Pollution
- Carbohydrate Chemistry and Synthesis
- Axial and Atropisomeric Chirality Synthesis
- Synthetic Organic Chemistry Methods
- Amino Acid Enzymes and Metabolism
- Biopolymer Synthesis and Applications
- Educational Systems and Policies
- Toxic Organic Pollutants Impact
- Synthesis and Reactions of Organic Compounds
- Alkaloids: synthesis and pharmacology
- Probiotics and Fermented Foods
- Bacterial Genetics and Biotechnology
- Carcinogens and Genotoxicity Assessment
Korea Research Institute of Chemical Technology
2015-2024
Konkuk University
2007-2015
Myongji University
2013
Fermentative production of cadaverine from renewable resources may support a sustainable biorefinery process to produce carbon-neutral nylons such as biopolyamide 510 (PA510). Cost-competitive is key factor in the successful commercialization PA510. In this study, an integrated biological and chemical involving biosynthesis, purification, its polymerization with sebacic acid was developed bio-PA510. To stably express ldcC Escherichia coli engineered Corynebacterium glutamicum PKC strain,...
Lignocellulosic raw materials have extensively been examined for the production of bio-based fuels, chemicals, and polymers using microbial platforms. Since xylose is one major components hydrolyzed lignocelluloses, it being considered a promising substrate in lignocelluloses based fermentation process. Ralstonia eutropha, most powerful natural producers polyhydroxyalkanoates (PHAs), has polymers. However, to best our knowledge, lignocellulosic feedstock not employed R. eutropha probably due...
Lignin valorization depends on microbial upcycling of various aromatic compounds in the form a complex mixture, including p-coumaric acid and ferulic acid. In this study, an engineered Pseudomonas putida strain utilizing lignin-derived monomeric via biological funneling was developed to produce 2-pyrone-4,6-dicarboxylic (PDC), which has been considered promising building block for bioplastics. The biosynthetic pathway PDC production established by introducing heterologous ligABC genes under...
To develop the risk prediction technology for mixture toxicity, a reliable and extensive dataset of experimental results is required. However, most published literature only provides data on combinations containing two or three substances, resulting in limited predicting toxicity complex mixtures. Complex mixtures may have different mode actions (MoAs) due to their varied composition, posing difficulty using conventional models, such as concentration addition (CA) independent action (IA)...
Recent interest has been focused on the production of platform chemicals from renewable biomass due to increasing concerns global warming and depletion fossil fuel reserves. Microbial in biorefineries suggested be a promising solution for these problems. Gamma-aminobutyrate (GABA), versatile bulk chemical used food pharmaceutical industry, is also as key monomer nylon 4. GABA can biologically produced by decarboxylation glutamate. In this study, we examined high glutamate-producing...
We report metabolic engineering of Corynebacterium glutamicum (C. glutamicum) for high-level production 5-hydroxyvaleric acid (5-HV), an important C5 platform chemical covering a wide range industrial applications, using glucose as sole carbon source. To derive 5-HV, artificial 5-HV biosynthesis pathway, composed the first three reaction steps l-lysine catabolic pathway via 5-aminovaleramide along with subsequent intracellular reduction step, was constructed: converted to glutarate...
γ-Aminobutyrate (GABA) is an important chemical by itself and can be further used for the production of monomer synthesis biodegradable polyamides. Until now, GABA usingCorynebacterium glutamicum harboring glutamate decarboxylases (GADs) has been limited due to discrepancy between optimal pH GAD activity (pH 4.0) cell growth 7.0). In this study, we developed recombinant C. strains expressing mutated from Escherichia coli (EcGADmut) GADs Lactococcus lactis CICC20209 (LlGAD) Lactobacillus...
Lysine decarboxylases (LDCs) from Escherichia coli, Lactobacillus saerimneri, Streptomyces coelicolor, Selemonas ruminantium, Hafnia alvei, and Vibrio vulnificus were examined for their ability to enhance the fermentative production of cadaverine in Corynebacterium glutamcium. Among these LDCs, plasmid-based expression H. alvei LDC gene (ldcCHa) under strong promoters (PH30, PH36) produced high concentrations (11.4–11.5 g/L), which is similar 12.5 g/L by E. coli (ldcCEc) PH30 promoter our...
A novel method for asymmetric synthesis of trans-2,3-disubstituted indolines has been developed. The strategy involves the (−)-sparteine-mediated electrophilic substitution 2-benzyl N-pivaloylaniline with aromatic or α,β-unsaturated aldehydes and subsequent intramolecular nucleophilic substitution. simple protocol two-step process can produce highly enantioenriched 3a–o up to 98:2 er.
Cadaverine is a C5 diamine monomer used for the production of bio-based polyamide 510. produced by decarboxylation l-lysine using lysine decarboxylase (LDC). In this study, we developed recombinant Escherichia coli strains expression LDC from Hafnia alvei. The resulting XBHaLDC strain was as whole cell biocatalyst high-level bioconversion into cadaverine without supplementation isopropyl β-d-1-thiogalactopyranoside (IPTG) induction protein and pyridoxal phosphate (PLP), key cofactor an...
An all-inclusive bio-chemical route from the fermentation process to downstream for C5 plasticizer synthesis was developed using fermentation-derived glutaric acid produced by metabolically engineered Corynebacterium glutamicum .
Corynebacterium glutamicum is an industrial strain used for the production of valuable chemicals such as L-lysine and L-glutamate. Although C. has various applications, a limited number tunable systems are available to engineer it efficient platform chemicals. Therefore, in this study, we developed novel promoter system based on repeats Vitreoscilla hemoglobin (Pvgb). Tunable expression green fluorescent protein (GFP) was investigated under one, four, eight Pvgb (Pvgb, Pvgb4, Pvgb8). The...
Several CoA transferases from Clostridium beijerinckii, C. perfringens and Klebsiella pneumoniae were examined for biosynthesis of lactate-containing polyhydroxyalkanoates (PHAs) in recombinant Escherichia coli XL1-Blue strain. The CB3819 gene the CB4543 pct K. pneumoniae, which encodes putative transferase gene, respectively, was co-expressed with Pseudomonas sp. MBEL 6-19 phaC1437 encoding engineered PHA synthase 1 (<TEX>$PhaC1_{Ps6-19}$</TEX>) to examine its activity construction key...
Biosynthesis of lactate-containing polyhydroxyalkanoates (PHAs) was examined in recombinant Escherichia coli W strain from sucrose. The Pseudomonas sp. MBEL 6-19 phaC1437 gene and the Clostridium propionicum pct540 gene, which encode engineered PHA synthase 1 (PhaC1 Ps6-19 ) C. propionyl-CoA transferase (Pct Cp ), respectively, were expressed E. to construct key metabolic pathway produce poly(3-hydroxybutyrate-co-lactate) [P(3HB-co-LA)]. expressing could synthesize P(3HB-co-13mol%LA) up...