A5007435746- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- biodegradable polymer synthesis and properties
- Bacterial Genetics and Biotechnology
- Enzyme Catalysis and Immobilization
- Enzyme Production and Characterization
- Viral Infectious Diseases and Gene Expression in Insects
- Protein purification and stability
- Bacteriophages and microbial interactions
- Cancer Research and Treatments
- Magnetic Bearings and Levitation Dynamics
- Electric Motor Design and Analysis
- Escherichia coli research studies
- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- Heat Transfer Mechanisms
- Microbial Metabolites in Food Biotechnology
- Monoclonal and Polyclonal Antibodies Research
- Advanced Adaptive Filtering Techniques
- Virus-based gene therapy research
- Magnetic Properties and Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Glycosylation and Glycoproteins Research
- Blind Source Separation Techniques
- Carbon dioxide utilization in catalysis
Korea Research Institute of Bioscience and Biotechnology
2013-2025
Kyung Hee University
1998-2024
Yonsei University
2001-2016
Pohang University of Science and Technology
2013-2015
Korea Advanced Institute of Science and Technology
1997-2010
Jeonbuk Development Institute
2009
Intelligent Synthetic Biology Center
2008
Korea Atomic Energy Research Institute
2003
Malonyl-CoA is an important central metabolite for the production of diverse valuable chemicals including natural products, but its intracellular availability often limited due to competition with essential cellular metabolism. Several malonyl-CoA biosensors have been developed high-throughput screening targets increasing pool. However, they are use only in Escherichia coli and Saccharomyces cerevisiae require multiple signal transduction steps. Here we report development a colorimetric...
Liver cancer is one of the leading causes death worldwide. To identify novel target genes that are related to liver carcinogenesis, we examined new differentially expressed in human hepatocellular carcinoma (HCC) cell lines and tissues based on sequence tag (EST) frequency. Eleven libraries were constructed from seven HCC three normal tissue samples obtained Korean patients. An analysis gene expression profiles for was performed using frequency ESTs these cDNA libraries. Genes identified...
Abstract Extracellular production of recombinant proteins in Escherichia coli has several advantages over cytoplasmic or periplasmic production. However, nonpathogenic laboratory strains E. generally excrete only trace amounts into the culture medium under normal growth conditions. Here we report a systematic proteome‐based approach for developing system high‐level extracellular . First, analyzed proteome an B strain, BL21(DE3), to identify naturally excreted proteins, assuming that these...
// Daejin Lim 1, 2 , Kwang Soo Kim Hyun-Ju Kyong-Cheol Ko 4 Jae Jun Song Jong Hyun Choi Minsang Shin 5 Jung-Joon Min 2, 3 Jae-Ho Jeong Hyon E. Choy 1 Department of Microbiology, Chonnam National University Medical School, Gwangju, Republic Korea Molecular Medicine, BK21 Plus, Graduate Nuclear Applied Microbiology Research Center, Bio-Materials Institute, Institute Bioscience and Biotechnology, Jeongeup, Jeonbuk, Kyungpook Daegu, Correspondence to: Choy, email: hyonchoy@jnu.ac.kr Min,...
Recent interest has been focused on the production of platform chemicals from renewable biomass due to increasing concerns global warming and depletion fossil fuel reserves. Microbial in biorefineries suggested be a promising solution for these problems. Gamma-aminobutyrate (GABA), versatile bulk chemical used food pharmaceutical industry, is also as key monomer nylon 4. GABA can biologically produced by decarboxylation glutamate. In this study, we examined high glutamate-producing...
Asthma is a chronic allergic respiratory disease lacking effective therapies. The present study investigated the anti‑asthmatic properties of lipid mediators using an ovalbumin (OVA)‑induced asthma model. Lipid (LM; 17S‑monohydroxy docosahexaenoic acid, resolvin D5 and protectin DX at ratio 3:47:50) were derived from acid through soybean lipoxygenase. LM treatment significantly alleviated major features asthma, including inflammatory cell infiltration, with particular reduction in...
Aspergillus terreus cadA, encoding cis-aconitate decarboxylase, is an essential gene for itaconic acid (IA) biosynthesis, but it primarily expressed as insoluble aggregates in most industrial hosts. This has been a hurdle the development of recombinant strategies IA production. Here, we created library synonymous codon variants (scv) cadA containing codons first 10 (except ATG) and screened Escherichia coli. Among positive clones, E. coli scvCadA_No8 showed more than 95% CadA soluble...
Abstract The fermentative production of platform chemicals in biorefineries is a sustainable alternative to current petroleum‐refining processes. Industrial microorganisms, such as Escherichia coli , Saccharomyces cerevisiae and Corynebacterium glutamicum, have been engineered microbial cell factories that are able utilize biomass for the value‐added polymers. Compared E. S. C. glutamicum displays weak carbon catabolite repression can co‐utilize mixed sugars sources, without any significant...
Although the tumor bulk is initially reduced by 5-fluorouracil (5-FU), chemoresistance developed due to prolonged chemotherapy in colorectal cancer (CRC). The enrichment of stem cells (CSCs) and infiltration tumor-associated macrophages (TAMs) contribute poor outcomes. A docosahexaenoic acid derivative our group, 7S,15R-dihydroxy-16S,17S-epoxy-docosapentaenoic (diHEP-DPA), exerts antitumor effects against TAMs CSCs previous study. current study aimed investigate whether diHEP-DPA was able...
The transcriptome profiles of recombinant Escherichia coli producing human insulin-like growth factor I fusion protein (IGF-I(f)) during the high-cell-density fed-batch culture were analyzed using DNA microarrays. expression levels 529 genes significantly altered after induction. About 200 down-regulated production IGF-I(f) Among these genes, we rationally selected and coexpressed in E. prsA gene (encoding a phosphoribosyl pyrophosphate synthetase) glpF glycerol transporter), which are...
The present study is conducted to investigate flow and heat/mass transfer characteristics in an impingement/effusion cooling system with crossflow. To simulate the system, two perforated plates are placed parallel staggered arrangements a gap distance of times hole diameter, initial crossflow passes between plates. Both injection effusion diameters 10 mm, Reynolds number based on diameter hole-to-hole pitch fixed 10,000 six respectively. effect crossflow, rate changed from 0.5 2 that...
<para xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> This paper deals with analysis and experiment of an axial flux permanent magnet (AFPM) brushless direct current (BLDC) motor minimized cogging torque. Recently, many optimal designs for the AFPM have been done by finite-element (FE) analysis, but such generally is time-consuming. In this study, equation magnetic lines existing between PMs cores assumed mathematically minimum torque calculated...
Recent studies using heterologous protein expression systems suggest that synonymous codons affect not only the but also properties of expressed protein. However, practical application this information is challenging, and to date, efforts employ bioinformatics tools design codon mixes have been marginally successful. Here, we sought enhance functional in Escherichia coli through completely random substitution first ten with codons, a previously isolated exocellulase CelEdx-SF301 as model...
A Gram-negative, motile and rod-shaped bacterial strain, BIO-TAS2-2(T), of the class Alphaproteobacteria, was isolated from a soil in Korea studied using polyphasic taxonomic approach. Strain BIO-TAS2-2(T) grew optimally at pH 7.5-8.5 30 degrees C presence 0-1.0 % (w/v) NaCl. neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain fell within clade comprising species genus Brevundimonas, forming coherent cluster with Brevundimonas terrae KSL-145(T) diminuta...