A5024384656- Silk-based biomaterials and applications
- Microbial Metabolic Engineering and Bioproduction
- Biochemical and Structural Characterization
- RNA and protein synthesis mechanisms
- Electrospun Nanofibers in Biomedical Applications
- Bacterial Genetics and Biotechnology
- Biofuel production and bioconversion
- Monoclonal and Polyclonal Antibodies Research
- Bacteriophages and microbial interactions
- Enzyme Catalysis and Immobilization
- Advanced Materials and Mechanics
- Antimicrobial Peptides and Activities
- Advanced Sensor and Energy Harvesting Materials
- Protein Hydrolysis and Bioactive Peptides
- Supramolecular Self-Assembly in Materials
- Viral Infectious Diseases and Gene Expression in Insects
- Cancer Research and Treatments
- Plant responses to water stress
- Plant Taxonomy and Phylogenetics
- Cellular Mechanics and Interactions
- Neurobiology and Insect Physiology Research
- Fungal Biology and Applications
- Enzyme Production and Characterization
- Polymer Surface Interaction Studies
- RNA Interference and Gene Delivery
Shanghai Jiao Tong University
2016-2025
Clemson University
2008-2025
Shandong University of Science and Technology
2021-2024
Tianjin Agricultural University
2022
Tufts University
2011-2016
Amarillo College
2012
Oak Ridge National Laboratory
2012
Korea Advanced Institute of Science and Technology
2008-2012
Intelligent Synthetic Biology Center
2008-2010
CAE (Canada)
2006
Spider dragline silk is a remarkably strong fiber that makes it attractive for numerous applications. Much has thus been done to make similar fibers by biomimic spinning of recombinant proteins. However, success limited in part due the inability successfully express native-sized proteins (250–320 kDa). Here we show 284.9 kDa protein spider Nephila clavipes produced and spun into displaying mechanical properties comparable those native silk. The protein, predominantly rich glycine (44.9%),...
Abstract Soft robots capable of efficiently implementing tasks in fluid-immersed environments hold great promise for diverse applications. However, it remains challenging to achieve robotization that relies on dynamic underwater adhesion and morphing capability. Here we propose the construction such with designer protein materials. Firstly, a resilin-like is complexed polyoxometalate anions form hydrogels can rapidly switch between soft adhesive stiff non-adhesive states aqueous response...
A four carbon linear chain diamine, putrescine (1,4-diaminobutane), is an important platform chemical having a wide range of applications in industry. Biotechnological production from renewable feedstock promising alternative to the synthesis that originates non-renewable petroleum. Here we report development metabolically engineered strain Escherichia coli produces at high titer glucose mineral salts medium. First, base was constructed by inactivating degradation and utilization pathways,...
Silk--elastin-like protein polymers (SELPs), consisting of the repeating units silk and elastin blocks, combine a set outstanding physical biological properties elastin. Because unique properties, SELPs have been widely fabricated into various materials for applications in drug delivery tissue engineering. However, little is known about fundamental self-assembly characteristics these remarkable polymers. Here we propose two-step process aqueous solution first time report importance ratio...
A five carbon linear chain diamine, cadaverine (1,5-diaminopentane), is an important platform chemical having many applications in industry. Bio-based production of from renewable feedstock a promising and sustainable alternative to the petroleum-based synthesis. Here, we report development metabolically engineered strain Escherichia coli that overproduces glucose mineral salts medium. First, degradation utilization pathways were inactivated. Next, L-lysine decarboxylase, which converts...
Elucidation of a genotype-phenotype relationship is critical to understand an organism at the whole-system level. Here, we demonstrate that comparative analyses multi-omics data combined with computational modeling approach provide framework for elucidating phenotypic characteristics organisms whose genomes are sequenced.We present comprehensive analysis genome-wide measurements incorporating multifaceted holistic - genome, transcriptome, proteome, and phenome determine differences between...
Abstract Self‐powered electronics using triboelectric nanogenerators (TENGs) is drawing increasing efforts and rapid advancements in eco/biocompatible energy harvesting, intelligent sensing, biomedical applications. Currently, the performances are mainly determined by pair materials’ inherent electron affinity difference, merely tuned chemical or physical methods, which significantly limit optional variety output capability, especially for natural‐biomaterial‐based TENGs. Herein, a...
Silk-elastin-like protein polymers (SELPs) combine the mechanical and biological properties of silk elastin. These have led to development various SELP-based materials for drug delivery. However, SELPs rarely been developed into nanoparticles, partially due complicated fabrication procedures, nor assessed potential as an anticancer delivery system. We recently constructed a series (SE8Y, S2E8Y, S4E8Y) with ratios elastin blocks described their capacity form micellar-like nanoparticles upon...
Background Some of the most frequent deficits seen in children with fetal alcohol spectrum disorders ( FASD ) and animal models are spatial memory impairments impaired executive functioning, which likely related to alcohol‐induced alterations hippocampus prefrontal cortex PFC ), respectively. Choline, a nutrient supplement, has been shown rat model ameliorate some alcohol's teratogenic effects, this effect may be mediated through choline's effects on DNA methylation. Methods Alcohol was...
The C6 dicarboxylic acid, adipic is an important platform chemical in industry. Biobased production of acid a promising alternative to the current petrochemical route. Here, we report biosynthesis using artificial pathway inspired by reversal beta-oxidation acids. biosynthetic comprises condensation acetyl-CoA and succinyl-CoA form backbone subsequent reduction, dehydration, hydrogenation, release from its thioester. was first tested vitro with reconstituted enzymes then functionally...
Abstract Flexible biocompatible mechanical energy harvesters are drawing increasing interest because of their high energy‐harvesting efficiency for powering wearable/implantable devices. Here, a type “self‐matched” tribo‐piezoelectric nanogenerators composed genetically engineered recombinant spider silk protein and piezoelectric poly(vinylidene fluoride) (PVDF)‐decorated poly(ethylene terephthalate) (PET) layers is reported. The PET layer serves as shared structure electrification both...
Elastomeric, robust, and biocompatible hydrogels are rare, while the need for these types of biomaterials in biomedical‐related uses remains high. Here, a new family genetically engineered silk–elastin‐like proteins (SELPs) with encoded enzymatic crosslinking sites is developed generation stimuli‐responsive yet robust hydrogels. Input into designs guided by simulation realized via genetic engineering strategies. The avoidance gamma irradiation or chemical during gel fabrication, lieu an...
Abstract Spiders achieve superior silk fibres by controlling the molecular assembly of proteins and hierarchical structure fibres. However, current wet-spinning process for recombinant spidroins oversimplifies natural spinning process. Here, water-soluble spider dragline protein (with a low weight 47 kDa) was adopted to prepare aqueous dope. Artificial silks were spun via microfluidic wet-spinning, using continuous post-spin drawing (WS-PSD). By mimicking apparatus, shearing elongational...
Abstract Silk protein fibres produced by silkworms and spiders are renowned for their unparalleled mechanical strength extensibility arising from high-β-sheet crystal contents as natural materials. Investigation of β-sheet-oriented conformational transitions in silk proteins at the nanoscale remains a challenge using conventional imaging techniques given limitations chemical sensitivity or limited spatial resolution. Here, we report on electron-regulated polymorphic revealed near-field...
The conserved microRNA396 (miR396) is involved in plant growth, development, and abiotic stress response multiple species through regulating its targets, Growth Regulating Factor (GRF) transcription factor genes. However, the role of miR396 has not yet been characterized perennial monocot species. In addition, molecular mechanism miR396-mediated remains unclear. To elucidate species, we generated transgenic creeping bentgrass (Agrostis stolonifera) overexpressing Osa-miR396c, a rice miRNA396...
Integrating multifunctionality such as stretchability, adhesiveness, and electroconductivity on a single protein hydrogel is highly desirable for various applications, remains challenge. Here we present the development of multifunctional hydrogels based resilin, natural rubber-like material with remarkable extensibility resilience. First, genetically engineered reslin-like proteins (RLPs) varying molecular weight were biosynthesized to tune mechanical strength stiffness cross-linked RLP...