A5013047009- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Enzyme Catalysis and Immobilization
- Amino Acid Enzymes and Metabolism
- Enzyme Structure and Function
- Bacterial Genetics and Biotechnology
- CRISPR and Genetic Engineering
- Enzyme Production and Characterization
- Gene Regulatory Network Analysis
- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- Microbial metabolism and enzyme function
- Plant biochemistry and biosynthesis
- Viral Infectious Diseases and Gene Expression in Insects
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Glycosylation and Glycoproteins Research
- Polyamine Metabolism and Applications
- Gut microbiota and health
- Christian Theology and Mission
- Microbial Community Ecology and Physiology
- Cancer Research and Treatments
- Innovative Microfluidic and Catalytic Techniques Innovation
- Biotin and Related Studies
- Biopolymer Synthesis and Applications
Korea Advanced Institute of Science and Technology
1994-2025
Korea Research Institute of Bioscience and Biotechnology
2016-2025
Korea University of Science and Technology
2016-2025
Chungnam National University
2008-2025
Intelligent Synthetic Biology Center
2011-2019
University of Science and Technology
2012-2016
Bioengineering Center
2015
Institut de Biologie systémique et synthétique
2012
Yuhan University
2012
Hoseo University
2010
Alkaline phosphatase (AP) catalyzes the hydrolytic cleavage of phosphate monoesters under alkaline conditions and plays important roles in microbial ecology molecular biology applications. Here, we report on first isolation biochemical characterization a thermolabile AP from metagenome.The gene encoding novel was isolated metagenomic library constructed with ocean-tidal flat sediments west coast Korea. The metagenome-derived (mAP) composed 1,824 nucleotides encodes polypeptide calculated...
The redox-dependent inhibition of thioredoxin (TRX) by thioredoxin-interacting protein (TXNIP) plays a pivotal role in various cancers and metabolic syndromes. However, the molecular mechanism this regulation is largely unknown. Here, we present crystal structure TRX–TXNIP complex demonstrate that TRX TXNIP mediated an intermolecular disulphide interaction resulting from novel bond-switching mechanism. Upon binding to TRX, undergoes structural rearrangement involves switching head-to-tail...
Repeat proteins have recently been of great interest as potential alternatives to immunoglobulin antibodies due their unique structural and biophysical features. We here present the development a binding scaffold based on variable lymphocyte receptors, which are nonimmunoglobulin composed Leucine-rich repeat modules in jawless vertebrates, by module engineering. A template was first constructed joining consensus between N- C-capping motifs receptors. The N-terminal domain redesigned...
The first line of defense in plants against pathogens is induced by the recognition microbe-associated molecular patterns (MAMP). Perception bacterial flagellin (flg22) pattern receptor flagellin-sensing 2 (FLS2) best characterized MAMP response, although underlying mechanisms are not fully understood. Here we studied relationship between salicylic acid (SA) or jasmonic (JA) signaling and FLS2-mediated monitoring flg22-triggered responses known SA JA related mutants Arabidopsis thaliana (L.)...
Biocatalytic cyclization is highly desirable for efficient synthesis of biologically derived chemical substances, such as the commodity chemicals ε-caprolactam and δ-valerolactam. To identify biocatalysts in lactam biosynthesis, we develop a caprolactam-detecting genetic enzyme screening system (CL-GESS). The Alcaligenes faecalis regulatory protein NitR adopted specific detection compounds against biosynthetic intermediates. We further systematically optimize components CL-GESS to enhance...
The gut microbiota is associated with diverse age-related disorders. Several rejuvenation methods, such as probiotic administration and faecal transplantation, have been applied to alter the microbiome promote healthy ageing. Nevertheless, prolongation of health span aged mice by remodelling remains challenging.Here, we report changes in microbial communities their functions mouse models during ageing three procedures including co-housing, serum-injection parabiosis. Our results showed that...
Although microRNA (miRNA) expression levels provide important information regarding disease states owing to their unique dysregulation patterns in tissues, translation of miRNA diagnostics into point-of-care (POC) settings has been limited by practical challenges. Here, we developed a hydrogel-based microfluidic platform for colorimetric profiling miRNAs, without the use complex external equipment fluidics and imaging. For sensitive reliable measurement risk sequence bias, employed gold...
Multiplex control of metabolic pathway genes is essential for maximizing product titers and conversion yields fuels, chemicals, pharmaceuticals in engineering. To achieve this goal, artificial transcriptional regulators, such as clustered regularly interspaced short palindromic repeats (CRISPR) interference (CRISPRi), have been developed to specifically repress interest.In study, we deployed a tunable CRISPRi system multiplex repression competing and, thus, directed carbon flux toward...
Large-scale screening of enzyme libraries is essential for the development cost-effective biological processes, which will be indispensable production sustainable biobased chemicals. Here, we introduce a genetic circuit termed Genetic Enzyme Screening System that highly useful high-throughput from diverse microbial metagenomes. The consists two AND logics. first logic, inputs are target and its substrate, responsible accumulation phenol compound in cell. Then, inducible transcription factor,...
Clustered regularly interspaced short palindromic repeats interference (CRISPRi) is an emerging technology for artificial gene regulation. Type II CRISPR-Cas endonuclease Cas9 the most widely used protein regulation with CRISPRi. Here, we present type V-A Cpf1-based We constructed l-rhamnose-inducible CRISPRi system DNase-deactivated Cpf1 from Eubacterium eligens (EedCpf1) and compared its performance catalytically deactivated Streptococcus pyogenes (SpdCas9). In contrast to SpdCas9, EedCpf1...
Interleukin-6 (IL-6) is a multifunctional cytokine that regulates immune responses for host defense and tumorigenic process. Upregulation of IL-6 known to constitutively phosphorylate signal transducer activator transcription 3 (STAT3), leading activation multiple oncogene pathways inflammatory cascade. Here, we present the development high-affinity protein binder, termed repebody, which effectively suppresses non–small cell lung cancer in vivo by blocking IL-6/STAT3 signaling. We selected...
(-)-α-Bisabolol, also known as levomenol, is an unsaturated sesquiterpene alcohol that has mainly been used in pharmaceutical and cosmetic products due to its anti-inflammatory skin-soothing properties. (-)-α-Bisabolol currently manufactured by steam-distillation of the essential oils extracted from Brazilian candeia tree under threat because natural habitat constantly shrinking. Therefore, microbial production (-)-α-bisabolol plays a key role development sustainable renewable...
Isoprene is a valuable precursor for synthetic rubber and signature product of terpenoid pathways. Here, we developed an isoprene biosensor by employing TbuT transcriptional regulator Ralstonia pickettii to express fluorescent reporter gene in response intracellular engineered Escherichia coli. The recognizes as its less-preferred effector molecule; thus, amplified the expression using T7 RNA polymerase-mediated cascade iteratively tuned promoter transcribing tbuT improve sensitivity...
• Methane conversion to a value-added mevalonate using methanotrophs as biocatalysts. A powerful and tunable synthetic biology toolkit for was developed. CRISPR base editor enabled the efficient markerless single-step genome editing. significantly high production of (2,089 mg/L) achieved. These versatile tools can realize methanotrophic biorefineries. Methanotrophs are promising sustainable cell factory platforms owing their ability convert most potent greenhouse gas, methane valuable...
Abstract Background As our understanding of gut microbiota’s metabolic impacts on health grows, the interest in engineered probiotics has intensified. This study aimed to engineer probiotic Escherichia coli Nissle 1917 (EcN) produce indoleacetic acid (IAA) response inflammatory biomarkers thiosulfate and nitrate. Results Genetic circuits were developed initiate IAA synthesis upon detecting signals, optimizing a heterologous biosynthetic pathway, incorporating RiboJ insulator enhance...
Flash-spun filaments (FSFs) made from high-density polyethylene (HDPE) are widely used in industrial nonwovens due to their unique morphology and mechanical robustness. In this study, we investigated the effect of polymer concentration (5-15 wt%) on FSF formation using a laboratory-scale flash-spinning system operating under supercritical conditions. Morphological, mechanical, crystallographic analyses were conducted understand underlying mechanisms. As increased, filament thickness,...