A5100676174- Immune Response and Inflammation
- Agriculture, Soil, Plant Science
- Immune Cell Function and Interaction
- Enzyme Structure and Function
- Diverse Approaches in Healthcare and Education Studies
- Glycosylation and Glycoproteins Research
- Metabolism and Genetic Disorders
- Immunotherapy and Immune Responses
- Plant Virus Research Studies
- Plant tissue culture and regeneration
- Technology and Data Analysis
- T-cell and B-cell Immunology
- Alcoholism and Thiamine Deficiency
- Electron and X-Ray Spectroscopy Techniques
- Genomics, phytochemicals, and oxidative stress
- Infectious Encephalopathies and Encephalitis
- Ecology and Conservation Studies
- Food composition and properties
- Advanced Electron Microscopy Techniques and Applications
- NF-κB Signaling Pathways
- Myofascial pain diagnosis and treatment
- Innovation in Digital Healthcare Systems
- Phytase and its Applications
- Protein Hydrolysis and Bioactive Peptides
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
Novarex (South Korea)
2022-2023
Institut Pasteur Korea
2014-2022
Kongju National University
2022
University of Seoul
2022
Korea Basic Science Institute
2020-2022
Stanford University
2018
Kyung Hee University
2013-2017
Korea Advanced Institute of Science and Technology
2003-2014
Korea Research Institute of Bioscience and Biotechnology
2003-2014
Kyungpook National University
2004-2013
Repeat proteins have recently been of great interest as potential alternatives to immunoglobulin antibodies due their unique structural and biophysical features. We here present the development a binding scaffold based on variable lymphocyte receptors, which are nonimmunoglobulin composed Leucine-rich repeat modules in jawless vertebrates, by module engineering. A template was first constructed joining consensus between N- C-capping motifs receptors. The N-terminal domain redesigned...
Monocyte chemoattractant protein-1 (MCP-1) is associated with various inflammatory diseases involving bone loss, and expressed along its receptor by marrow-derived macrophages (BMM), which are osteoclast (OC) precursors. To investigate the role of MCP-1 in remodeling, we compared MCP-1-knockout (KO) mice wild-type (WT) mice. The absence increased mass lowered serum collagen type I fragments (CTX-1) TRACP 5b, but had no significant effect on N-terminal propeptide procollagen, suggesting that...
Abstract Human monocytes and neutrophils play major roles in clearing bacteria from human blood tissues. We found that the herpes virus entry mediator (HVEM) was highly expressed neutrophils, its interaction with “homologous to lymphotoxins, shows inducible expression, competes simplex glycoprotein D for HVEM/tumor necrosis factor (TNF)-related 2” (LIGHT) enhanced bactericidal activity against Listeria monocytogenes Staphylococcus aureus. The LIGHT-HVEM increased levels of phagocytosis,...
Genistein, a soy‐derived isoflavone, inhibits growth of tumor cells from various malignancies. Here we investigated the effect genistein on cervical cancer (HeLa and CaSki) its possible mechanism. Genistein significantly suppressed cell HeLa CaSki at concentrations 20 60 μmol/L, respectively, for 24 h. Western blotting analysis showed that reduced phosphorylation AKT extracellular signal–regulated kinase (ERK)‐1/2 induced p38 mitogen‐activated protein (MAPK) c‐Jun N‐terminal (JNK). Moreover,...
Genistein, a naturally occurring isoflavonoid abundant in soy products, has anticancer activity multiple tumor cells. In this study, we evaluated the apoptotic effect of genistein on cervical cancer cells and its mechanism apoptosis. Genistein inhibited proliferation (HeLa, CaSki, C33A). HeLa were most sensitive to genistein, whereas CaSki C33A less sensitive. Sub‐G 1 analysis showed that increased up 45% at concentration 60 μmol/L cells, it produced 21% 17% respectively, same concentration....
4-1BB costimulates T cells to carry out effector functions such as eradication of established tumours. (CD137) is a member the TNF receptor family, and its triggering by either ligand or antibody ligation induces T-cell activation growth. We analysed tumour-infiltrating lymphocytes (TIL) in experimental B16F10 melanoma model determine mechanisms involved 4-1BB-mediated tumour suppression. 4-1BB(+/+) mice survived longer than 4-1BB(-/-) mice, survival was further prolonged with an agonistic...
Thiamine pyrophosphate (TPP) is an essential cofactor for various pivotal cellular processes in all living organisms, including bacteria. biosynthesis occurs bacteria but not humans; therefore, the enzymes this pathway are attractive targets antibiotic development. Among these enzymes, thiamine monophosphate kinase (ThiL) catalyzes final step of pathway, phosphorylating to produce TPP. Here, we extensively investigated ThiL Pseudomonas aeruginosa, a major pathogen responsible...
The tumor necrosis factor receptor family molecule 4-1BB (CD137) has diverse roles in adaptive and innate immune responses. However, little is known of its role bacterial infections. Previously, we showed that 4-1BB-deficient mice have enhanced susceptibility to Listeria monocytogenes infection, pretreated with agonistic anti-4-1BB antibody (3E1) were much more resistant L. infection than treated control antibody. In this study, report stimulating by administering 3E1 the early phase...
Repeat proteins are increasingly attracting much attention as alternative scaffolds to immunoglobulin antibodies due their unique structural features. Nonetheless, engineering interaction interface and understanding molecular basis for affinity maturation of repeat still remain a challenge. Here, we present structure-based rational design protein with high binding target protein. As model protein, Toll-like receptor4 (TLR4) decoy receptor composed leucine-rich (LRR) modules was used, its...
Repeat proteins have recently attracted much attention as alternative scaffolds to immunoglobulin antibodies due their unique structural and biophysical features. In particular, repeat show high stability against temperature chaotic agents. Despite many studies, features for the of remain poorly understood. Here we present an interesting result from in silico analyses pursuing factors which affect proteins. Previously developed repebody structure based on variable lymphocytes receptors...
ABSTRACT Severe sepsis and septic shock caused mainly by bacterial infections are life-threatening conditions that urge the development of novel therapies. However, host responses to pathophysiology have not been clearly understood, which remains a major obstacle for effective therapeutics. Recently, we shown stimulation costimulatory molecule, CD137, enhanced survival mice infected with Gram-positive (G + ) intracellular bacterium Listeria monocytogenes but decreased in polymicrobial model....
4-1BB (CD137), a member of the tumor necrosis factor receptor superfamily, is expressed on activated T-cells, and signaling due to interaction with ligand or ligation anti-4-1BB monoclonal antibody (mAb) costimulates T cells. It has been shown that administration mAb induces anti-tumor immunity in mice, but nature cellular subsets responsible for this uncertain. In study we found B16F10 melanoma-bearing mice induced marked expansion CD11c+CD8+ T-cells parallel suppression pulmonary tumors....
The therapeutic efficacy and in vivo biological function of a glycoprotein is significantly affected by its glycosylation profile. For the development glycoproteins with applications, selection cell lines producing adequate glycoform crucial. Here, we demonstrate an array-based analysis secreted for rapid efficient single desirable glycosylation. Our approach relies on microengraving interrogation produced individual cells microwell array terms profile as well amount. On basis statistical...
ABSTRACT Sepsis, a leading cause of death worldwide, involves proinflammatory responses and inefficient bacterial clearance. Previously, we have shown that CD137 (4-1BB), member the tumor necrosis factor receptor superfamily, plays critical roles in eradicating infective Listeria monocytogenes , gram-positive bacterium, stimulation protects mice from sepsis-induced death. In this study, unexpectedly found activation aggravated polymicrobial sepsis due to mixed gram-negative infection induced...
Rabies virus relies on cellular machinery for its replication while simultaneously evading the host immune response. Despite their importance, little is known about key factors required rabies infection. Here, we focused human kinome, at core of many pathways, to unveil a new understanding infectious cycle and discover potential therapeutic targets in small interfering RNA screening. The mitogen-activated protein kinase pathway phosphatidylinositol metabolism were identified as prominent...
Abstract Quorum sensing is a cell–cell communication mechanism that involved in the regulation of biological functions such as luminescence, virulence, and biofilm formation. Quorum‐quenching enzymes, which interrupt quorum‐sensing signaling through degradation molecules, have emerged new approach to controlling preventing bacterial virulence pathogenesis. In an effort develop quorum‐quenching enzymes with improved catalytic activities, genetic circuit system based on acylhomoserine‐lactone...
Halocidin is an antimicrobial peptide found in the tunicate. A series of experiments were previously conducted attempt to develop a novel antibiotic derived from halocidin, as was determined evidence profound activity against variety antibiotic-resistant microbes, with significantly less toxicity human blood cells. In this study, we assessed validity one halocidin congeners, called Khal, new for treatment systemic bacterial infections. Our vitro tests showed that MICs Khal several...
Herpes virus entry mediator (HVEM) is a member of the TNF receptor (TNFR) superfamily and expressed on many immune cells, including T B NK monocytes, neutrophils. Interaction HVEM with its ligand, LIGHT, costimulates cells increases bactericidal activity monocytes The interaction recruits cytoplasmic TNFR-associated factor adaptor proteins to intracellular domain HVEM. This leads NFkappaB activation as result IkappaBalpha degradation and/or JNK/AP-1 activation, ultimately results in...