Phenethyl isothiocyanate (PEITC), a constituent of edible cruciferous vegetables such as watercress, not only affords significant protection against chemically induced cancer in experimental rodents but also inhibits growth human cells by causing apoptotic and autophagic cell death. However, the underlying mechanism PEITC-induced death is fully understood. Using LNCaP PC-3 prostate model, we demonstrate that initiated production reactive oxygen species (ROS) resulting from inhibition...

BackgroundEfficacy of withaferin A (WA), an Ayurvedic medicine constituent, for prevention mammary cancer and its associated mechanisms were investigated using mouse tumor virus–neu (MMTV-neu) transgenic model.

Current dogma favors elimination of therapy-resistant cancer stem cells for chemoprevention breast cancer. We showed recently that mammary development in a transgenic mouse model (mouse tumor virus-neu; MMTV-neu) was inhibited significantly upon treatment with withaferin A (WA), steroidal lactone derived from medicinal plant. Herein, we demonstrate the prevention by WA is accompanied vivo suppression (bCSC). In vitro mammosphere formation dose-dependently MCF-7 and SUM159 human cells. Other...

Celecoxib, the first US FDA-approved selective cyclooxygenase-2 (COX-2) inhibitor initially developed for treatment of adult rheumatoid arthritis and osteoarthritis, was reported to reduce polyp burden in patients with familial adenomatous polyposis. This specific COX-2 also protects against experimentally induced carcinogenesis, but molecular mechanisms underlying its chemopreventive activities remain largely unresolved. In present work, we found that celecoxib inhibited...

Benzyl isothiocyanate (BITC), a constituent of edible cruciferous vegetables, inhibits growth breast cancer cells but the mechanisms underlying inhibitory effect BITC are not fully understood. Here, we demonstrate that treatment causes FoxO1-mediated autophagic death in cultured human cells. The BITC-treated (MDA-MB-231, MCF-7, MDA-MB-468, BT-474, and BRI-JM04) MDA-MB-231 xenografts from mice exhibited several features characteristic autophagy, including appearance double-membrane vacuoles...

Increased de novo synthesis of fatty acids is a rather unique and targetable mechanism human prostate cancer. We have shown previously that oral administration sulforaphane (SFN) significantly inhibits the incidence and/or burden prostatic intraepithelial neoplasia well-differentiated adenocarcinoma in TRansgenic Adenocarcinoma Mouse Prostate (TRAMP) mice. The present study used cellular models cancer archived plasma/adenocarcinoma tissues sections from TRAMP to demonstrate inhibition acid...

Despite advances in therapeutic approaches, the five-year survival rate for head and neck squamous cell carcinoma (HNSCC) patients is still less than fifty percent. Research has indicated that consumption of Allium vegetables or processed garlic containing diallyl trisulfide (DATS) can lower risk multiple types cancer. Nevertheless, effectiveness underlying mechanisms DATS against HNSCC have not been thoroughly explored until current study. In this research, it was found notably curtailed...

Genistein, a soy‐derived isoflavone, inhibits growth of tumor cells from various malignancies. Here we investigated the effect genistein on cervical cancer (HeLa and CaSki) its possible mechanism. Genistein significantly suppressed cell HeLa CaSki at concentrations 20 60 μmol/L, respectively, for 24 h. Western blotting analysis showed that reduced phosphorylation AKT extracellular signal–regulated kinase (ERK)‐1/2 induced p38 mitogen‐activated protein (MAPK) c‐Jun N‐terminal (JNK). Moreover,...

A small subset of mammary tumor-initiating cells (also known as breast cancer stem cells; bCSC), characterized by expression different markers [CD44(high)/CD24(low)/epithelial-specific antigen (ESA)+], aldehyde dehydrogenase-1 (ALDH1) activity, and ability to form mammospheres under ultra-low attachment culture conditions, are suspected evade conventional therapies leading disease recurrence. Elimination both therapy-sensitive epithelial tumor therapy-resistant bCSC is therefore necessary...

A nontoxic chemopreventive intervention efficacious against different subtypes of breast cancer is still a clinically unmet need. The present study was undertaken to determine the efficacy an Ayurvedic medicine phytochemical (Withaferin A, [WA]) for chemoprevention and elucidate its mode action.Chemopreventive WA (4 8 mg/kg body weight) determined using rat model induced by N-methyl-N-nitrosourea (MNU; n = 14 control group, 15 4 18 group). mechanisms underlying were elucidated...

Abstract d,l-Sulforaphane (SFN), a synthetic analogue of cruciferous vegetable–derived l-isomer, inhibits the growth human prostate cancer cells in culture and vivo retards development transgenic mouse model cancer. We now show that SFN treatment causes transcriptional repression androgen receptor (AR) LNCaP C4-2 at pharmacologic concentrations. Exposure to resulted concentration-dependent time-dependent decrease protein levels total AR as well Ser210/213-phosphorylated AR. The SFN-mediated...

We study how a machine based on deep learning algorithms learns Krylov spread complexity in quantum systems with N x random Hamiltonians drawn from the Gaussian unitary ensemble. Using thermofield double states as initial conditions, we demonstrate that convolutional neural network-based algorithm successfully across all timescales, including late-time plateaus where appear nearly featureless and random. Performance strongly depends basis choice, performing well energy eigenbasis or but...

Genistein, a naturally occurring isoflavonoid abundant in soy products, has anticancer activity multiple tumor cells. In this study, we evaluated the apoptotic effect of genistein on cervical cancer cells and its mechanism apoptosis. Genistein inhibited proliferation (HeLa, CaSki, C33A). HeLa were most sensitive to genistein, whereas CaSki C33A less sensitive. Sub‐G 1 analysis showed that increased up 45% at concentration 60 μmol/L cells, it produced 21% 17% respectively, same concentration....

Abstract We have shown previously that garlic constituent diallyl trisulfide (DATS) inhibits growth of cultured and xenografted human prostate cancer cells in association with apoptosis induction, but the mechanism cell death is not fully understood. The present study systematically investigates role inhibitor (IAP) family proteins regulation DATS-induced using PC-3 LNCaP dorsolateral from control DATS-treated transgenic adenocarcinoma mouse (TRAMP) mice. Level X-linked (XIAP) protein was...

Withaferin A (WA) is a promising phytochemical exhibiting in vitro and vivo anticancer activities against prostate other cancers, but the mechanism of its action not fully understood. In this study, we performed RNA-seq analysis using 22Rv1 human cancer cell line to identify mechanistic targets WA. Kyoto Encyclopedia Genes Genomes pathway differentially expressed genes showed most significant enrichment associated with metabolism. These results were validated LNCaP cells Hi-Myc transgenic...

Larissa Akemi Kido, Eun-Ryeong Hahm, Su-Hyeong Kim, Andressa Mara Baseggio, Valeria Helena Alves Cagnon, Shivendra V. Singh, Mário Roberto Maróstica Jr.. J Cancer Prev 2020;25:87-99. https://doi.org/10.15430/JCP.2020.25.2.87

Abstract We have shown previously that cruciferous vegetable constituent benzyl isothiocyanate (BITC) suppresses viability of cultured MCF-7 and MDA-MB-231 human breast cancer cells retards mammary development in MMTV-neu mice by causing apoptosis, but the mechanism cell death is not fully understood. now show whereas p53 dispensable for BITC-induced death, proapoptotic response to this promising chemopreventive agent mediated suppression X-linked inhibitor apoptosis (XIAP) protein...