A5022522651- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- Anaerobic Digestion and Biogas Production
- Gas Sensing Nanomaterials and Sensors
- Genomics and Phylogenetic Studies
- Advanced Chemical Sensor Technologies
- Advanced Thermoelectric Materials and Devices
- Advanced materials and composites
- RNA and protein synthesis mechanisms
- Gut microbiota and health
- ZnO doping and properties
- Microbial Fuel Cells and Bioremediation
- Microbial metabolism and enzyme function
- Clostridium difficile and Clostridium perfringens research
- Graphene research and applications
- Thermal properties of materials
- Analytical Chemistry and Sensors
- Thermal Radiation and Cooling Technologies
- CRISPR and Genetic Engineering
- Bacterial Genetics and Biotechnology
- High Entropy Alloys Studies
- Diet and metabolism studies
- Algal biology and biofuel production
- Advanced Sensor and Energy Harvesting Materials
- Solar-Powered Water Purification Methods
Korea Institute of Industrial Technology
2020-2025
Massachusetts Institute of Technology
2023-2024
Korea Advanced Institute of Science and Technology
2014-2023
Hanyang University
2016-2021
Ansan University
2017-2018
Government of the Republic of Korea
2017-2018
Intelligent Synthetic Biology Center
2013
Among CO 2 -fixing metabolic pathways in nature, the linear Wood–Ljungdahl pathway (WLP) phylogenetically diverse acetate-forming acetogens comprises most energetically efficient pathway, requires least number of reactions, and converts to formate then into acetyl-CoA. Despite two genes encoding glycine synthase being well-conserved WLP gene clusters, functional role under autotrophic growth conditions has remained uncertain. Here, using reconstructed genome-scale model i SL771 based on...
Monodispersed nano Pt catalyzed linear dense assembly of SWCNTs bundles on a noble multi-layered electrode system for cost-effective hydrogen sensor.
The gut microbiota is associated with diverse age-related disorders. Several rejuvenation methods, such as probiotic administration and faecal transplantation, have been applied to alter the microbiome promote healthy ageing. Nevertheless, prolongation of health span aged mice by remodelling remains challenging.Here, we report changes in microbial communities their functions mouse models during ageing three procedures including co-housing, serum-injection parabiosis. Our results showed that...
Acetogenic bacteria use cellular redox energy to convert CO2 acetate using the Wood-Ljungdahl (WL) pathway. Such can be derived from electrons generated H2 as well inorganic materials, such photoresponsive semiconductors. We have developed a nanoparticle-microbe hybrid system in which chemically synthesized cadmium sulfide nanoparticles (CdS-NPs) are displayed on cell surface of industrial acetogen Clostridium autoethanogenum The converts into without need for additional sources, H2, and...
Acetogenic bacteria are a unique biocatalyst that highly promises to develop the sustainable bioconversion of carbon oxides (e.g., CO and 2 ) into multicarbon biochemicals. Genotype–phenotype relationships important for engineering their metabolic capability enhance biocatalytic performance; however, systemic investigation on fitness contribution individual gene has been limited. Here, we report genome-scale CRISPR interference screening using 41,939 guide RNAs designed from E. limosum...
Acetogens are naturally capable of metabolizing carbon monoxide (CO), a component synthesis gas (syngas), for autotrophic growth in order to produce biomass and metabolites such as acetyl-CoA via the Wood-Ljungdahl pathway. However, acetogens is often inhibited by presence high CO concentrations because toxicity, thus limiting their biosynthetic potential industrial applications. Herein, we implemented adaptive laboratory evolution (ALE) improvement Eubacterium limosum ATCC 8486 under...
Eubacterium limosum is one of the important bacteria in C1 feedstock utilization as well human gut microbiota. Although E. has recently garnered much attention and investigation on a genome-wide scale, bottleneck for systematic engineering lack available genetic tools an efficient genome editing platform. To overcome this limitation, we here report expanded CRISPR-Cas9 system. We have developed inducible promoter system that enables implementation to precisely manipulate target genes...
Exploiting biological processes to recycle renewable carbon into high value platform chemicals provides a sustainable and greener alternative current reliance on petrochemicals. In this regard Cupriavidus necator H16 represents particularly promising microbial chassis due its ability grow wide range of low-cost feedstocks, including the waste gas dioxide, whilst also naturally producing large quantities polyhydroxybutyrate (PHB) during nutrient-limited conditions. Understanding complex...
Power generation technologies based on water movement and evaporation use water, which covers more than 70% of the Earth’s surface can also generate power from moisture in air. Studies are conducted to diversify materials increase performance validate energy mechanisms. In this study, a water-based generator was fabricated by coating cellulose acetate with carbon black. To optimize generator, Fourier-transform infrared spectroscopy, specific area, zeta potential, particle size, electrical...
Autotrophic conversion of CO2 to value-added biochemicals has received considerable attention as a sustainable route replace fossil fuels. Particularly, anaerobic acetogenic bacteria are naturally capable reducing or CO various metabolites. To fully utilize their biosynthetic potential, an understanding acetogenesis-related genes and regulatory elements is required. Here, we completed the genome sequence syngas fermenting Eubacterium limosum ATCC 8486 determined its transcription start sites...
Inkjet-printed electronics using metal particles typically lack electrical conductivity and interfacial adhesion with an underlying substrate. To address the inherent issues of printed materials, this Research Article introduces advanced materials processing methodologies. Enhanced inkjet-printed copper (Cu) on a flexible polyimide film is achieved by new surface modification technique, nanostructured self-assembled monolayer (SAM) (3-mercaptopropyl)trimethoxysilane. A standardized test...
Abstract Background Acetogenic bacteria constitute promising biocatalysts for the conversion of CO 2 /H or synthesis gas (H /CO/CO ) into biofuels and value-added biochemicals. These microorganisms are naturally capable autotrophic growth via unique acetogenesis metabolism. Despite their biosynthetic potential commercial applications, a systemic understanding transcriptional translational regulation metabolism remains unclear. Results By integrating genome-scale transcriptomic translatomic...
RNA sequencing techniques have enabled the systematic elucidation of gene expression (RNA-Seq), transcription start sites (differential RNA-Seq), transcript 3′ ends (Term-Seq), and post-transcriptional processes (ribosome profiling). The main challenge transcriptomic studies is to remove ribosomal RNAs (rRNAs), which comprise more than 90% total in a cell. Here, we report low-cost robust bacterial rRNA depletion method, RiboRid, based on enzymatic degradation by thermostable RNase H. This...
Nitrogen doped TiO2 (N-TiO2) nanoparticles were synthesized via a novel plasma enhanced electrolysis method using bulk titanium (Ti) as source material and nitric acid the nitrogen dopant. This possesses remarkable merits with regard to direct-metal synthesis of its one-step process, eco-friendliness, ability be mass produced. The from Ti metal dipped in 5 – 15 mmol electrolyte under application AC 500 V, minimum range voltage generate plasma. By controlling concentration, nanoparticle size...
Global lithium demand is expected to increase from 500,000 tons in 2021 2 million 2030, and the shortage reach 220,000 tons. This dramatic largely due widespread use of ion batterie, or electronic devices. In recent years, there has been a global effort extract ions dissolved seawater wastewater. We also developed extraction system consisting an anion exchange membrane (AEM) lanthanum titanium oxide (LLTO) membrane. The produced concentrated solutions with concentration 10,000 ppm...