A5068494013- Antibiotic Resistance in Bacteria
- Bacterial Genetics and Biotechnology
- Escherichia coli research studies
- Bacterial biofilms and quorum sensing
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Vibrio bacteria research studies
- CRISPR and Genetic Engineering
- Bacterial Infections and Vaccines
- Genomics and Phylogenetic Studies
- Cancer Research and Treatments
- Virus-based gene therapy research
- Neuroinflammation and Neurodegeneration Mechanisms
- Agriculture, Soil, Plant Science
- Trace Elements in Health
- bioluminescence and chemiluminescence research
- Immune cells in cancer
- Nanoplatforms for cancer theranostics
- Microbial Metabolic Engineering and Bioproduction
- Cancer Mechanisms and Therapy
- Phagocytosis and Immune Regulation
- Heme Oxygenase-1 and Carbon Monoxide
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Lipid Membrane Structure and Behavior
- DNA and Nucleic Acid Chemistry
Kyungpook National University
2016-2025
University of Washington
2025
Oregon State University
2025
Chonnam National University Hospital
2015
Kyoto University
2010-2014
Chonnam National University
2001-2014
Korea Center for Disease Control and Prevention
2012
Kangwon National University
2005
Seoul National University
2005
There is a growing appreciation for fundamental connection between lipid metabolism and the immune response. Macrophage phagocytosis signature innate response to pathogen exposure, cytoplasmic membrane expansion required engulf phagocytic target. The sterol regulatory element binding proteins (SREBPs) are key transcriptional that sense intracellular environment modulate expression of genes fatty acid cholesterol maintain homeostasis. In this study, we show TLR4-dependent stimulation...
Bacterial cancer therapies aim to manipulate bacteria effectively deploy therapeutic payloads tumors. Attenuated alone often cannot eradicate solid Salmonella can be engineered deliver cytotoxic drugs either trigger an immune response or increase antitumor efficacy when combined with chemotherapeutic drugs. However, the extracellular matrix (ECM) surrounding cells forms a barrier that limits ability of and penetrate eliminate To overcome this limitation, we developed strategy combine...
Transcription initiation by RNA polymerase (RNP) carrying the house-keeping σ subunit, 70 (Eσ ), is repressed H-NS at a number of promoters including hdeAB p in Escherichia coli , while with RNP stationary phase σ, 38 not. We investigated molecular mechanism selective repression to identify differences transcription two forms RNPs, which show indistinguishable promoter selectivities vitro. Using as model promoter, we observed purified components that H-NS, acting sequence centered -118,...
We have examined expression of the genes on Salmonella pathogenicity island 1 (SPI1) during growth under physiologically well defined standard condition Luria-Bertani medium with aeration. found that central regulator hilA and its control are expressed at onset stationary phase. Interestingly, two-component regulatory hilC/hilD, sirA/barA, ompR, which known to modulate from promoter (hilAp) so-called "inducing conditions" (Luria-Bertani containing 0.3 m NaCl without aeration), acted...
Significance Bacteria can repel invader DNA and RNA molecules by using an adaptive immunity mechanism called clustered regularly interspaced short palindromic repeats (CRISPRs)-Cas. CRISPR loci in a host genome are repository of fragments obtained from previous encounters with invader, which be transcribed activated into (crRNA) sequences complementary to or RNA. In some CRISPR-Cas systems, crRNA is assembled targeting complex “Cascade” that seeks form R-loop triggers recruitment...
The spread of multidrug-resistant Acinetobacter baumannii in hospitals and nursing homes poses serious healthcare challenges. Therefore, we aimed to isolate characterize lytic bacteriophages targeting carbapenem-resistant (CRAB). Of the 21 isolated A. phages, 11 exhibited potent activities against clinical isolates CRAB. Based on host spectrum RAPD-PCR results, phages were categorized into four groups. Three (vB_AbaP_W8, vB_AbaSi_W9, vB_AbaSt_W16) further characterized owing their...
Abstract Sterol regulatory element-binding protein (SREBP)-1c is involved in cellular lipid homeostasis and cholesterol biosynthesis highly increased nonalcoholic steatohepatitis (NASH). However, the molecular mechanism by which SREBP-1c regulates hepatic stellate cells (HSCs) activation NASH animal models patients have not been fully elucidated. In this study, we examined role of regulation LCN2 gene expression. Wild-type knockout (1cKO) mice were fed a high-fat/high-sucrose diet, treated...
Acinetobacter baumannii is a challenging multidrug-resistant pathogen in healthcare. Phage vB_AbaSi_W9 (GenBank: PP146379.1), identified our previous study, shows lytic activity against 26 (89.66%) of 29 carbapenem-resistant (CRAB) strains with various sequence types (STs). It promising candidate for CRAB treatment; however, its efficiency insufficient complete bacterial lysis. Therefore, this study aimed to investigate the clinical utility phage by identifying antimicrobial agents that show...
// Daejin Lim 1, 2 , Kwang Soo Kim Hyun-Ju Kyong-Cheol Ko 4 Jae Jun Song Jong Hyun Choi Minsang Shin 5 Jung-Joon Min 2, 3 Jae-Ho Jeong Hyon E. Choy 1 Department of Microbiology, Chonnam National University Medical School, Gwangju, Republic Korea Molecular Medicine, BK21 Plus, Graduate Nuclear Applied Microbiology Research Center, Bio-Materials Institute, Institute Bioscience and Biotechnology, Jeongeup, Jeonbuk, Kyungpook Daegu, Correspondence to: Choy, email: hyonchoy@jnu.ac.kr Min,...
A comprehensive cognitive science requires broad sampling of human behavior to justify general inferences about the mind. For example, field psycholinguistics relies on a rich history comparative study, with many available resources that systematically document languages. Surprisingly, despite longstanding interest in questions universality and diversity, psychology music has few such resources. Here, we report Expanded Natural History Song Discography, an open-access corpus vocal (n = 1007...
Acanthamoeba is an opportunistic pathogen responsible for granulomatous amoebic encephalitis and keratitis. Despite its clinical significance, effective treatments remain challenging due to a limited understanding of pathogenic mechanism. This study developed genetic manipulation system in facilitate gene function drug screening studies. We applied the Cre/loxP integrate encoding tdTomato fluorescent protein into genome castellanii via homologous recombination. The polyubiquitin untranslated...
Being distinct from bacteria and eukaryotes, Archaea constitute a third domain of living things. The DNA replication, transcription, translation machineries are more similar to those whereas the genes involved in metabolic processes show similarity their bacterial counterparts. We report here that TK0471/TrmB-like 2 (TrmBL2), addition histone, is novel type abundant chromosomal protein model euryarchaeon Thermococcus kodakarensis . chromosome T. can be separated into regions enriched either...
Abstract Background Acinetobacter baumannii is an important opportunistic pathogen responsible for various nosocomial infections. The BfmRS two-component system plays a role in pathogenesis and antimicrobial resistance of A. via regulation bacterial envelope structures. This study investigated the sensor kinase, BfmS, localization outer membrane protein A (OmpA) production vesicles (OMVs) using wild-type ATCC 17978, Δ bfmS mutant, -complemented strains. Results mutant showed hypermucoid...
Most clinical isolates of Acinetobacter baumannii , a nosocomial pathogen, are multidrug-resistant (MDR), fueling the search for alternative therapies. Bacteriophage-derived endolysins have potent antibacterial activities and considered as alternatives to antibiotics against A. infection. Gram-negative bacteria possess outer lipid membrane that prevents direct contact between cell wall. We hypothesized fusion antimicrobial peptide (AMP) with endolysin could help reduce bacterial resistance...
Treatment of infections caused by Acinetobacter baumannii nosocomial strains has become increasingly problematic owing to their resistance antibiotics. ppGpp is a secondary messenger involved in growth control and various stress responses bacteria. The mechanism for inhibition antibiotic via still unidentified pathogenic bacteria including A. baumannii. Here, we investigated the effects on efflux pump (EP)-related genes baumannii.ppGpp-deficient -complementary were constructed conjugation...
Caveolar endocytosis has an important function in the cellular uptake of some bacterial toxins, viruses, and circulating proteins. However, molecular machinery involved caveolae-dependent is poorly defined. In present study, we identify a new mechanism for caveolin-1–dependent entry Salmonella into host cells via direct regulation actin reorganization. contrast to interaction caveolae with other pathogens, did not form Salmonella-containing vesicles or endosomes cells. Instead, rapidly moved...
Acinetobacter baumannii expresses various virulence factors to adapt hostile environments and infect susceptible hosts. This study investigated the regulatory network of BfmRS two-component AbaIR quorum sensing (QS) systems in expression virulence-associated genes A. ATCC 17978. The ΔbfmS mutant exhibited a significant decrease surface motility, which presumably resulted from low pilT A1S_0112-A1S_0119 gene cluster. ΔbfmR displayed reduction biofilm pellicle formation due csu operon....
Summary In the modern concept of gene regulation, ‘ DNA looping’ is most common underlying mechanism in interaction between RNA polymerase ( RNAP ) and transcription factors acting at a distance. This study demonstrates an additional by which ‐bound proteins communicate with each other, analysing bacterial histone‐like nucleoid‐structuring protein H‐NS ), general transcriptional silencer. The LEE5 promoter p enteropathogenic Escherichia coli was used as model system to investigate ‐mediated...
Metal ions at the active site of an enzyme act as cofactors, and their dynamic fluctuations can potentially influence activity. Here, we use λ-exonuclease a model with two Mg2+ binding sites probe activity various concentrations magnesium by single-molecule-FRET. We find that while MgA2+ MgB2+ have similar constants, dissociation rate is order magnitude lower than due to kinetic-barrier-difference. At physiological concentration, ion near 5'-terminal side scissile phosphate dissociates...