A5076565717- Mesenchymal stem cell research
- Nerve injury and regeneration
- High-Energy Particle Collisions Research
- Chinese history and philosophy
- Particle physics theoretical and experimental studies
- Muscle Physiology and Disorders
- Pneumonia and Respiratory Infections
- Japanese History and Culture
- Immunodeficiency and Autoimmune Disorders
- Antimicrobial Resistance in Staphylococcus
- Quantum Chromodynamics and Particle Interactions
- Neurogenesis and neuroplasticity mechanisms
- Respiratory viral infections research
- Tissue Engineering and Regenerative Medicine
- MicroRNA in disease regulation
- Monoclonal and Polyclonal Antibodies Research
- Streptococcal Infections and Treatments
- Pluripotent Stem Cells Research
- Pneumocystis jirovecii pneumonia detection and treatment
- Hereditary Neurological Disorders
- Philippine History and Culture
- Glycosylation and Glycoproteins Research
- Autophagy in Disease and Therapy
- Genetic Neurodegenerative Diseases
- Bacterial biofilms and quorum sensing
Kyungpook National University
2024-2025
Ewha Womans University
2016-2024
University of Massachusetts Chan Medical School
2023-2024
Pusan National University
2023-2024
Seoul National University
2003-2023
Ewha Womans University Medical Center
2017
Brigham and Women's Hospital
2013-2016
Harvard University
2013-2016
University of Alabama at Birmingham
2007-2014
Davidson College
2014
We have recently reported a new pneumococcal serotype (6C), which is closely related to 6A (I. H. Park et al., J. Clin. Microbiol. 45:1225-1233, 2007). To investigate the genetic basis for 6C, we studied capsule gene loci of 14 6C isolates from three different continents, including one isolated in Alabama 27 years ago. The wciN region all has 1,029-bp-long sequence that replaces 1,222-bp-long region. This recombination event created 1,125-bp-long open reading frame encodes product also...
Staphylococcus aureus is a leading cause of superficial and invasive human disease that often refractory to antimicrobial therapy. Vaccines have the potential reduce morbidity, mortality, economic impact associated with staphylococcal infections. However, single-component vaccines targeting S. failed show efficacy in clinical trials.A novel glycoengineering technology for creation multicomponent vaccine described. Genes encoding capsular polysaccharide (CP) biosynthesis, PglB (a...
Since the 23-valent pneumococcal polysaccharide vaccine (PPV23) is less effective for older adults than young adults, it important to investigate immunologic basis reduced efficacy of PPV23 among adults. We determined effectiveness (n = 55) and 44) by measuring serum IgG, IgM, IgA concentrations opsonic capacities against serotypes 14, 18C, 23F. While showed no difference in levels IgG antibodies (PPS), had lower IgM antibody all three serotypes. In both age groups, anti-PPS or were much...
Stem cells are regarded as an important source of which may be used to promote the regeneration skeletal muscle (SKM) has been damaged due defects in organization tissue caused by congenital diseases, trauma or tumor removal. In particular, mesenchymal stem (MSCs), require less invasive harvesting techniques, represent a valuable for cell therapy. present study, we demonstrated that human tonsil-derived MSCs (T-MSCs) differentiate into myogenic vitro and transplantation myoblasts myocytes...
Schwann cells (SCs), which produce neurotropic factors and adhesive molecules, have been reported previously to contribute structural support guidance during axonal regeneration; therefore, they are potentially a crucial target in the restoration of injured nervous tissues. Autologous SC transplantation has performed shown promising clinical results for treating nerve injuries donor site morbidity, insufficient production considered as major issue. Here, we differentiation tonsil-derived...
Charcot-Marie-Tooth (CMT) disease is the most common hereditary peripheral neuropathy. CMT type 1A (CMT1A) accounts for approximately 50% of patients and linked to PMP22 gene duplication. Histone deacetylase-6 (HDAC6) has pleiotropic effects, such as regulating lipid homeostasis cellular stress. Although HDAC6 been regarded a promising drug target neurodegenerative diseases, its inhibition not yet tested in CMT1A. Here we have therapeutic potential CKD-504, clinical stage inhibitor, mouse...
Abstract The vaccine elicitation of HIV tier-2-neutralization antibodies has been a challenge. Here, we report the isolation and characterization CD4-binding site (CD4bs) specific monoclonal antibody, HmAb64, from human volunteer immunized with polyvalent DNA prime-protein boost vaccine. HmAb64 is derived heavy chain variable germline gene IGHV1-18 light IGKV1-39. It third complementarity-determining region (CDR H3) 15 amino acids. On cross-clade panel 208 HIV-1 pseudo-virus strains,...
BACKGROUND: Recently, human embryonic stem (hES) cells have become very important resources for basic research on cell replacement therapy and other medical applications. The purpose of this study was to test whether pluripotent hES lines could be successfully derived from frozen–thawed embryos that were destined discarded after 5 years in a routine IVF‐embryo transfer programme an STO feeder layer can used the culture cells. METHODS: Donated frozen (blastocysts or pronuclear) thawed,...
Developmental capacity of human multipronuclear (PN) zygotes cryopreserved using an ultra-rapid freezing method and electron microscope (EM) grids was studied. Multipronuclear obtained from a IVF programme were used as alternative to normal 2PN zygotes; they divided into 3PN or >or =4PN their in-vitro development cryo-injury compared according PN number. EFS30, which consisted 30% ethylene glycol, 18% Ficoll, 0. 5 mol/l sucrose 10% fetal bovine serum with added modified Dulbecco's phosphate...
Treatment of Staphylococcus aureus infections has become increasingly difficult because the emergence multidrug-resistant isolates. Development a vaccine to prevent staphylococcal remains priority. To determine whether clumping factor A (ClfA) is good target protein for inclusion in multivalent vaccine, we evaluated its efficacy variety relevant infection models, challenging with different S. strains. ClfA adsorbed Alhydrogel and mixed Sigma Adjuvant System was more immunogenic stimulated...
Mesenchymal stem cells (MSCs) are capable of self-renewal and differentiation thus a valuable source for the replacement diseased or damaged organs. Previously, we reported that tonsils can be an excellent reservoir MSCs regeneration skeletal muscle (SKM) damage. However, mechanisms involved in from tonsil-derived (T-MSCs) to myocytes via myoblasts remain unclear. To clarify these mechanisms, analyzed gene expression profiles T-MSCs during into compared with human (hSKMCs). Total RNA was...
Abstract Placenta mesenchymal stem cells (PMSCs) have the characteristic features of including renewability in vitro, surface expression, differentiation potency and ability to adhere culture surface. PMSCs expressed genes are normally found embryonic tissues before onset gastrulation, indicating multipotency. However, stemness can depend on stages placenta from which were isolated. isolated two different for comparison, that is first third trimesters. Both sets had very similar patterns...
Long Live the Tributary System!The Future of Studying East Asian Foreign Relations Saeyoung Park Keywords international relations, Westphalian system, Sinocentric world order 中華世界秩序, IR turn in history, David Kang, Asia before West, China's rise, hegemony, Sino-speak, imperialism Almost a half century has passed since publication John King Fairbank's edited volume, The Chinese World Order.1 Since then, tributary system come to serve as something synecdoche for pre-1900 relations. Briefly,...
Abstract Laser-induced graphene (LIG) is a three-dimensional structure fabricated through the irradiation of polymer substrate with laser energy (or fluence, equivalently). This methodology offers cost-effective and facile means producing 3D nanostructures, yielding materials characterized by extremely high surface area superior electrical properties, rendering them advantageous for various electrochemical applications. Nonetheless, it imperative to acknowledge that structures material...
Charcot-Marie-Tooth disease (CMT) is a hereditary with heterogeneous phenotypes and genetic causes. CMT type 1A (CMT1A) of affecting the peripheral nerves caused by duplication myelin protein 22 (PMP22) gene. Human tonsil-derived mesenchymal stem cells (TMSCs) are useful for cell therapy in various diseases can be differentiated into Schwann cell-like (TMSC-SCs). We investigated potential TMSC-SCs called neuronal regeneration-promoting (NRPCs) nerve muscle regeneration C22 mice, model CMT1A....
Streptococcus pneumoniae, a major human pathogen, expresses at least 91 serologically distinct carbohydrate capsules. Since pneumococcal vaccines are designed to elicit antibodies against many different capsular polysaccharides (PSs), it is important identify the epitopes involved in eliciting anti-capsular PS antibodies. We investigated recognized by Dob1, which hybridoma-secreting immunoglobulin G2 antibody of serotype 6B (Y. Sun et al., Infect. Immun. 67:1172-1179, 1999). found that Dob1...
Staphylococcal infections are a major source of global morbidity and mortality. Currently there exists no antistaphylococcal vaccine in clinical use. Previous animal studies suggested possible role for purified lipoteichoic acid as target eliciting protective IgG to several Gram-positive pathogens. Since the highly conserved (poly)glycerolphosphate backbone is antigenic humoral immune system during staphylococcal infections, we developed synthetic method producing glycerol phosphoramidites...
The capsular polysaccharide (CP) produced by Staphylococcus aureus is a virulence factor that allows the organism to evade uptake and killing host neutrophils. Polyclonal antibodies serotype 5 (CP5) type 8 (CP8) polysaccharides are opsonic protect mice against experimental bacteremia provoked encapsulated staphylococci. Thus, passive immunotherapy using CP has been considered for prevention or treatment of invasive antibiotic-resistant S. infections. In this report, we generated monoclonal...
Most Staphylococcus aureus isolates produce either a serotype 5 (CP5) or 8 (CP8) capsular polysaccharide, and the CP antigens are targets for vaccine development. Since CP5 CP8 have similar trisaccharide repeating units, it is important to identify an epitope shared by both CP8. To characterize cross-reactivity between CP8, immunogenicity of conjugate vaccines in mice rabbits was evaluated serological assays. Immune sera were also tested functional activity vitro opsonophagocytic-killing...