A5048142450- Pneumonia and Respiratory Infections
- SARS-CoV-2 and COVID-19 Research
- Bacterial Infections and Vaccines
- COVID-19 Clinical Research Studies
- Respiratory viral infections research
- Advanced Glycation End Products research
- Pneumocystis jirovecii pneumonia detection and treatment
- Bioinformatics and Genomic Networks
- Cancer Genomics and Diagnostics
- Microbial Fuel Cells and Bioremediation
- Malaria Research and Control
- Immune Response and Inflammation
- Monoclonal and Polyclonal Antibodies Research
- Gene expression and cancer classification
- Animal Virus Infections Studies
- Immunotherapy and Immune Responses
- Supercapacitor Materials and Fabrication
- Mosquito-borne diseases and control
- Long-Term Effects of COVID-19
- Gene Regulatory Network Analysis
- Head and Neck Cancer Studies
- MicroRNA in disease regulation
- SARS-CoV-2 detection and testing
- Chromatin Remodeling and Cancer
- Electrochemical sensors and biosensors
Yonsei University
2011-2025
Gangnam Severance Hospital
2021-2025
Samsung (South Korea)
2011-2024
Sungkyunkwan University
2024
Samsung Changwon Hospital
2024
Severance Hospital
2022
Yeungnam University
2020-2021
Inje University Busan Paik Hospital
2015-2020
Andong National University
2018
Pusan National University
2016-2017
Using two monoclonal antibodies, we found subtypes among pneumococcal isolates that are typed as serotype 6A by the quellung reaction. The prevalent subtype bound to both antibodies and was labeled here 6Aalpha, whereas minor only one antibody 6Abeta. To determine biochemical nature of serologically defined subtypes, purified capsular polysaccharides (PSs) from examined their chemical structures with gas-liquid chromatography mass spectrometry. study results for 6Aalpha PS consistent...
Abstract Purpose: Medulloblastoma, a malignant pediatric brain tumor, is incurable in about one third of patients despite multimodal treatments. In addition, current therapies can lead to long-term disabilities. Based on studies the extensive tropism neural stem cells (NSC) toward gliomas and secretion growth factors common glioma medulloblastoma, we hypothesized that NSCs could target medulloblastoma be used as cellular therapeutic delivery system. Experimental Design: The migratory ability...
We have recently reported a new pneumococcal serotype (6C), which is closely related to 6A (I. H. Park et al., J. Clin. Microbiol. 45:1225-1233, 2007). To investigate the genetic basis for 6C, we studied capsule gene loci of 14 6C isolates from three different continents, including one isolated in Alabama 27 years ago. The wciN region all has 1,029-bp-long sequence that replaces 1,222-bp-long region. This recombination event created 1,125-bp-long open reading frame encodes product also...
Nuclear protein HMGB1 is secreted in response to various stimuli and functions as a danger-associated molecular pattern. Extracellular induces inflammation, cytokine production, immune cell recruitment via activation of receptors. As does not contain an endoplasmic reticulum-targeting signal peptide, the reticulum-Golgi independently unconventional secretion pathway. However, mechanism underlying remains largely unknown. Here, we investigated role secretory autophagy machinery vesicular...
Abstract Purpose: Brainstem gliomas are usually inoperable and have a dismal prognosis. Based on the robust tropisms of neural stem cells (NSC) mesenchymal (MSC) to brain tumors, we compared tumor-tropic migratory capacities these evaluated therapeutic potential genetically engineered human NSCs encoding cytosine deaminase (CD) IFNβ against brainstem gliomas. Experimental Design: The directed MSCs glioma (F98) were both in vitro vivo. (HB1.F3) various MSCs, such as bone marrow–derived...
ABSTRACT Although virulence of Streptococcus pneumoniae is associated with its capsule, some pathogenic S. isolates lack capsules and are serologically nontypeable (NT). We obtained 64 that were identified as NT “pneumococci” (i.e., bacteria satisfying the conventional definition but without multilocus sequence typing [MLST]-based ) by traditional criteria. All optochin sensitive had lytA , 63 ply . Twelve cpsA suggesting presence a defective capsular polysaccharide synthesis ( cps locus....
The nuclear protein HMGB1 (high mobility group box 1) is secreted by monocytes-macrophages in response to inflammatory stimuli and serves as a danger-associated molecular pattern. Acetylation phosphorylation of are implicated the regulation its nucleocytoplasmic translocation for secretion, although known induce H2O2 production. Here we show that H2O2-induced oxidation HMGB1, which results formation an intramolecular disulfide bond between Cys23 Cys45, necessary sufficient secretion....
With the increasing use of culture-expanded mesenchymal stromal cells (MSCs) for cell therapies, factors that regulate cellular characteristics MSCs have been major interest. Oxygen concentration has shown to influence functions MSCs, as well other normal and malignant stem cells. However, underlying mechanisms hypoxic responses precise role hypoxia-inducible factor-1α (Hif-1α), master regulatory protein hypoxia, in remain unclear, due limited span Hif-1α stabilization complex network...
High-mobility group box 1 (HMGB1), a well-known danger-associated molecular pattern molecule, acts as pro-inflammatory molecule when secreted by activated immune cells or released after necrotic cell damage. HMGB1 binds to immunogenic bacterial components and augments septic inflammation. In this study, we show how mediates complement activation, promoting sterile We that activates the classical pathway of system in an antibody-independent manner binding C1q. The C3a activation product human...
ABSTRACT Streptococcus pneumoniae clinical isolates were recently described that produced capsular polysaccharide with properties of both serotypes 6A and 6B. Their hybrid serological property correlated mutations affecting the glycosyltransferase WciP, which links rhamnose to ribitol by an α(1-3) linkage for 6C α(1-4) 6B 6D. The had in triad residues WciP have been enzyme specificity. canonical are Ala192-Ser195-Arg254 Ser192-Asn195-Gly254 To prove responsible serotype, we introduced...
High mobility group box-1 (HMGB1) is involved in various diseases and associated with the resistance of many types human cancers to chemotherapy; however, its role cancer metastasis remains unexplored. This study examined HMGB1 status both highly poorly metastatic cells response genotoxic stress. The weakly mouse melanoma cell lines (B16 vs. B16-F10), (SK-MEL-28 SK-MEL-24), colon (DLD-1 LS174T), wild-type (WT) knockout (KO) embryonic fibroblasts (MEFs) were treated doxorubicin (Dox)...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the etiological agent of COVID-19, causes life-threatening disease. This novel enters host cells via tract, promoting formation severe pulmonary lesions and systemic Few animal models can simulate clinical signs pathology COVID-19 patients. Diverse preclinical studies using K18-hACE2 mice Syrian golden hamsters, which are highly permissive to SARS-CoV-2 in emerging; however, pathogenesis cellular tropism these remain obscure. We...
Purpose We evaluated serum procalcitonin (PCT) as a diagnostic marker of neonatal sepsis, and compared PCT levels with C-reactive protein (CRP) levels. Methods retrospectively reviewed the medical records 269 neonates suspected infection, admitted to Wonkwang University School Medicine & Hospital between January 2011 December 2012, for whom CRP values had been obtained. Neonates were categorized into 4 groups according infection severity. analyzed compared, their effectiveness markers was...
Since nasopharyngeal carriage of pneumococcus precedes invasive pneumococcal disease, characteristics isolates could be incorrectly assumed to reflect those isolates. While most pneumococci express a capsular polysaccharide, nontypeable are sometimes isolated. Carriage nontypeables tend encode novel surface proteins in place polysaccharide synthetic locus, the cps locus. In contrast, is believed indispensable for and from population-based disease surveillance have not been extensively...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection induces excessive pro-inflammatory cytokine release and cell death, leading to organ damage mortality. High-mobility group box 1 (HMGB1) is one of the damage-associated molecular patterns that can be secreted by stimuli, including viral infections, its secretion levels are related a variety inflammatory diseases. Here, aim study was show SARS-CoV-2 induced HMGB1 via active passive release. Active mediated...
The coronavirus pandemic has accelerated the development of next-generation vaccination technology to combat future outbreaks. Mucosal effectively protects mucosal surfaces, primary sites viral entry, by inducing secretion immunoglobulin A (IgA) and humoral IgG. Here, a dissolving microneedle (DMN) is adopted as vaccine delivery platform directly penetrate sublingual site, which rich in antigen-presenting cells (APCs) lymphoid tissues. (SLDMN) comprised micropillar-based compartment...
Cancer stem cells (CSCs), a subpopulation in tumors, are known to cause drug resistance, tumor recurrence and metastasis. Based on the characteristic formation of mammospheres vitro conditions, mammosphere assay has become an essential tool for quantifying CSC activity breast cancer research. However, manual counting is time-consuming process that not amenable high-throughput screening, there occasional inaccuracies determining diameter. In this study, we proposed novel automated method...
Abstract Alzheimer’s disease (AD) is a progressive neurodegenerative associated with complex genetic etiology. Besides the apolipoprotein E ε4 ( APOE ε4) allele, few dozen other loci AD have been identified through genome-wide association studies (GWAS) conducted mainly in individuals of European ancestry. Recently, several GWAS performed ethnic groups shown importance replicating that identify previously established risk and searching for novel loci. -stratified yielded might be masked by,...
Abstract Background C1q has been reported to reveal complement-independent roles in immune and non-immune cells. binds its specific receptors regulate distinct functions that rely on the environment cell types. Discoidin domain receptor 2 (DDR2) is activated by collagen wound healing controlling matrix metalloproteinase (MMP) expression. Since exhibits a collagen-like structure, we hypothesized might engage DDR2 extracellular (ECM) remodeling. Methods Cell-based assay, proximity ligation...