A5074612002- Genomics and Phylogenetic Studies
- Enzyme Production and Characterization
- Microbial Community Ecology and Physiology
- Enzyme Structure and Function
- Salmonella and Campylobacter epidemiology
- Molecular Biology Techniques and Applications
- Bacterial Genetics and Biotechnology
- Microbial Metabolites in Food Biotechnology
- DNA Repair Mechanisms
- Biofuel production and bioconversion
- Bacteriophages and microbial interactions
- Vibrio bacteria research studies
- Protist diversity and phylogeny
- Pneumonia and Respiratory Infections
- Redox biology and oxidative stress
- Food Quality and Safety Studies
- Algal biology and biofuel production
- Microbial Metabolism and Applications
- Plant Pathogens and Fungal Diseases
- Cancer Research and Treatments
- Microbial Metabolic Engineering and Bioproduction
- Photosynthetic Processes and Mechanisms
- Neonatal and Maternal Infections
- Streptococcal Infections and Treatments
- Aquaculture disease management and microbiota
Korea Atomic Energy Research Institute
2016-2025
Korea University of Science and Technology
2017-2025
Republic of Korea Army
2025
Seoul National University
2001-2013
Methicillin‑resistant Staphylococcus aureus (MRSA) is difficult to treat using available antibiotic agents. Honeybee venom has been widely used as an oriental treatment for several inflammatory diseases and bacterial infections. The contains predominantly biologically active compounds, however, the therapeutic effects of such materials when MRSA infections have not investigated extensively. present study evaluated bee its principal component, melittin, in terms their antibacterial activities...
The main protease (Mpro) is a major having an important role in viral replication of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), novel that caused pandemic 2020. Here, active Mpro was obtained as 34.5 kDa protein by overexpression E. coli BL21 (DE3). optimal pH and temperature were 7.5 37 °C, respectively. displayed Km value 16 μM with Dabcyl-KTSAVLQ↓SGFRKME-Edans. Black garlic extract 49 polyphenols studied for their inhibitory effects on purified Mpro. IC50 values 137...
We have examined expression of the genes on Salmonella pathogenicity island 1 (SPI1) during growth under physiologically well defined standard condition Luria-Bertani medium with aeration. found that central regulator hilA and its control are expressed at onset stationary phase. Interestingly, two-component regulatory hilC/hilD, sirA/barA, ompR, which known to modulate from promoter (hilAp) so-called "inducing conditions" (Luria-Bertani containing 0.3 m NaCl without aeration), acted...
Abstract Berberine, an isoquinoline plant alkaloid, has been known to generate a wide variety of biochemical and pharmacological effects. In order elucidate the molecular mechanism for berberine‐induced enhancement radio‐sensitization, human hepatoma HepG2 cells were treated with berberine combined irradiation. The anti‐tumor effect gamma radiation was found be significantly enhanced by berberine. evidences apoptosis, such as apoptotic DNA fragmentation annexin V staining, observed in...
Brief CommunicationLancefield group B streptococci (GBS), also referred to as Streptococcus agalactiae, is a gram-positive, opportunistic pathogen that colonizes the gastrointestinal and genitourinary tracts of up 50% healthy adults [1][2][3].In 1938, it was first identified human pathogen, causing fatal puerperal sepsis [4], but remained relatively unknown sporadic asymptomatic cases were reported until 1960s.By 1970s, GBS had emerged predominant septicemia meningitis in neonates infants...
Bacterial cell-to-cell communication, termed quorum sensing (QS), controls bacterial behavior by using various signal molecules. Despite the fact that LuxS/autoinducer-2 (AI-2) QS system is necessary for normal expression of Salmonella pathogenicity island-1 (SPI-1), mechanism remains unknown. Here, we report LsrR protein, a transcriptional regulator known to be involved in LuxS/AI-2-mediated QS, also associated with regulation SPI-1-mediated virulence. We determined negatively SPI-1 and...
The basidiomycetous fungus Cryptococcus neoformans has been known to be highly radiation resistant and found in fatal radioactive environments such as the damaged nuclear reactor at Chernobyl. To elucidate mechanisms underlying resistance phenotype of C. neoformans, we identified genes affected by gamma through genome-wide transcriptome analysis characterized their functions. We that involved DNA damage repair systems were upregulated response radiation. Particularly, deletion recombinase...
Deinococcus radiodurans shows extreme resistance to a range of remarkable environmental stresses. Deinococcal exopolysaccharide (DeinoPol) is component the cell wall, but its role in stress has not yet been well-described. In this study, we isolated and characterized DeinoPol from R1 strain investigated application as an antioxidant agent. Bioinformatic analysis indicated that dra0033, encoding ExoP-like protein, was involved biosynthesis, dra0033 mutation significantly decreased survival...
A Gram-stain-positive, strictly aerobic, spherical, non-motile red-pigmented bacterial strain, designated MJ27(T), was isolated from a sludge sample of the Daejeon sewage disposal plant in South Korea. polyphasic approach used to study taxonomic position strain MJ27(T). Strain MJ27(T) shared highest 16S rRNA gene sequence similarity with Deinococcus grandis DSM 3963(T) (98.8 %), caeni Ho-08(T) (97.5 %) and aquaticus PB314(T) (96.6 %.); levels type strains other species were less than 96.0 %....
We present a single molecule visualization approach for the quantitative analysis of reactive oxygen species (ROS) induced DNA damage, such as base oxidation and stranded breaks in large molecules. utilized Fenton reaction to generate damage with subsequent enzymatic treatment using mixture three types glycosylases remove oxidized bases, then fluorescent labeling on damaged lesions via nick translation. This analytical platform provided capability count one or two sites per λ (48.5 kb),...
Lignocellulosic biomass has long been recognized as a potential sustainable source of sugars for biofuels. However, many physicochemical structural and compositional factors inhibit the enzymatic digestibility lignocellulosic biomass. In this study, efficient pretreatment method rice straw (RS) was developed RS hydrolysate applied in cultivation microalgae lipid production.Gamma ray irradiation (GRI) alkali solution were used pretreatment, saccharification carried out with lignocellulolytic...
Streptococcus agalactiae (group B Streptococcus, GBS) is a leading cause of severe invasive disease in neonate, elderly, and immunocompromised patients worldwide. Despite recent advances the diagnosis intrapartum antibiotic prophylaxis (IAP) GBS infections, it remains one most common causes neonatal morbidity mortality, causing serious infections. Furthermore, studies reported an increasing number infections pregnant women elderly. Although IAP effective, has several limitations, including...
Orthohantavirus hantanense (HTNV) poses a substantial global public health threat due to its role in causing hemorrhagic fever with renal syndrome (HFRS). HTNV outbreaks are particularly prevalent the Gyeonggi and Gangwon Provinces of Republic Korea (ROK). This study aimed evaluate application advanced nanopore sequencing bioinformatics generate complete genome sequences HTNV, objective accurately identifying infection sources analyzing their genetic diversity. In 2022 2023, we collected 579...
A Gram-staining-positive, strictly aerobic, spherical, non-motile, red-pigmented bacterium, designated strain MK03(T), was isolated from a soil sample collected in South Korea. The taxonomic position of the novel investigated using polyphasic approach. In phylogenetic analyses based on 16S rRNA gene sequences, MK03(T) placed clade formed by members genus Deinococcus family Deinococcaceae and appeared to be most closely related aerolatus 5516T-9(T) (97.4% sequence similarity), marmoris...
Genome instability is detrimental for living things because it induces genetic disorder diseases and transfers incorrect genome information to descendants. Therefore, organisms have evolutionarily conserved signaling networks sense repair DNA damage. However, how the damage response pathway regulated maintaining integrity of fungal pathogens this contributes their pathogenicity remain elusive. In study, we investigated in basidiomycete pathogen Cryptococcus neoformans , which causes...