Gram-negative bacteria produce outer membrane vesicles that play a role in the delivery of virulence factors to host cells. However, little is known about membrane-derived (MVs) produced by Gram-positive bacteria. The present study examined production MVs from Staphylococcus aureus and investigated cells subsequent cytotoxicity. Four S. strains tested, two type clinical isolates, spherical nanovesicles during vitro culture. were also vivo infection isolate mouse pneumonia model. Proteomic...

Lytic bacteriophages (phages) have been investigated as treatments for bacterial infectious diseases. An induced phage, SAP-26, was isolated from a clinical isolate of Staphylococcus aureus. It belongs to the family Siphoviridae and its genome consists double-stranded 41,207 bp DNA coding 63 open reading frames. The phage SAP-26 showed wide spectrum lytic activity against both methicillin-resistant S. aureus methicillin-susceptible S.aureus. Furthermore, combined treatment with antimicrobial...

Multidrug-resistant (MDR) bacteria are a major public-health concern. Bacteriophage endolysins (lysins) can be used as novel antimicrobial agents against bacterial infections. In this study, endolysin (LysSS) containing lysozyme-like domain was evaluated for its antibacterial activity various species of bacteria. The LysSS-encoding gene analyzed and cloned the LysSS recombinant protein expressed purified. Purified to determine in vitro measure protection rate Acinetobacter baumannii systemic...

The collection of whole microbial communities (bacteria, archaea, fungi, and viruses) together constitutes the gut microbiome. Diet, age, stress, host genetics, diseases cause increases or decreases in relative abundance diversity bacterial species (dysbiosis). We aimed to investigate composition at different taxonomic levels healthy controls (HCs) with active Crohn’s disease (CD), ulcerative colitis (UC), ischemic (IC) using culture- non-culture-based approaches identify biomarkers...

The spread of multidrug-resistant Acinetobacter baumannii in hospitals and nursing homes poses serious healthcare challenges. Therefore, we aimed to isolate characterize lytic bacteriophages targeting carbapenem-resistant (CRAB). Of the 21 isolated A. phages, 11 exhibited potent activities against clinical isolates CRAB. Based on host spectrum RAPD-PCR results, phages were categorized into four groups. Three (vB_AbaP_W8, vB_AbaSi_W9, vB_AbaSt_W16) further characterized owing their...

Acinetobacter baumannii is a challenging multidrug-resistant pathogen in healthcare. Phage vB_AbaSi_W9 (GenBank: PP146379.1), identified our previous study, shows lytic activity against 26 (89.66%) of 29 carbapenem-resistant (CRAB) strains with various sequence types (STs). It promising candidate for CRAB treatment; however, its efficiency insufficient complete bacterial lysis. Therefore, this study aimed to investigate the clinical utility phage by identifying antimicrobial agents that show...

We isolated a new lytic Pseudomonas aeruginosa phage that requires type IV pili for infection. PA1Ø has broad bactericidal spectrum, covering Gram-positive and Gram-negative bacteria, can eradicate biofilm cells. may be developed as therapeutic agent biofilm-related mixed infections with P. Staphylococcus aureus.

The traditional markerless gene deletion technique based on overlap extension PCR has been used for generating deletions in multidrug-resistant Acinetobacter baumannii. However, the method is time-consuming because it requires restriction digestion of products DNA cloning and construction new vectors containing a suitable antibiotic resistance cassette selection A. baumannii merodiploids. Moreover, availability sites recombinant bacteria harboring desired chimeric plasmid are limited, making...

ObjectivesTo investigate the differences in antimicrobial susceptibility and resistance mechanisms against imipenem between Acinetobacter baumannii genomic species 13TU.

ABSTRACT Screening of 368 consecutive nonreplicate clinical isolates Enterobacteriaceae resistant to nalidixic acid and at least one extended-spectrum β-lactam revealed the presence qnrA , qnrB qnrS determinants, identified novel variants, in Citrobacter freundii isolates. This study also revealed, for first time, linkage armA and/or AmpC-type β-lactamase genes on large conjugative plasmids.

ABSTRACT Acinetobacter baumannii is a Gram-negative bacterium causing nosocomial infections worldwide. To gain quick insight into the molecular basis of biofilm formation in A. , we determined complete genome sequence strain 1656-2, which forms sturdy and resistant to multiple drugs.

Multidrug-resistant (MDR) bacteria are a major threat to public health. Bacteriophage endolysins (lysins) promising alternative treatment traditional antibiotics. However, the lysins currently under development still underestimated. Herein, we cloned lysin from SAP-26 bacteriophage genome. The recombinant LysSAP26 protein inhibited growth of carbapenem-resistant Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa, oxacillin-resistant Staphylococcus...

Most clinical isolates of Acinetobacter baumannii , a nosocomial pathogen, are multidrug-resistant (MDR), fueling the search for alternative therapies. Bacteriophage-derived endolysins have potent antibacterial activities and considered as alternatives to antibiotics against A. infection. Gram-negative bacteria possess outer lipid membrane that prevents direct contact between cell wall. We hypothesized fusion antimicrobial peptide (AMP) with endolysin could help reduce bacterial resistance...

Three bacterial strains, B5-R-101 T , TA-R-1 and BL-R-1 were isolated from the feces of a healthy Korean individual. Cells these strains Gram-stain-positive, facultatively anaerobic, oxidase-negative, catalase-positive, rod-shaped, non-motile. They able to grow within temperature range 10–42°C (optimum, 32–37°C), at pH 2.0–10.0 5.5–8.0), NaCl concentration 0.5–10.5% (w/v). All three exhibited 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities ranging 58 ± 1.62 79 1.46% (%...

Journal Article CTX-M and SHV-12 β-lactamases are the most common extended-spectrum enzymes in clinical isolates of Escherichia coli Klebsiella pneumoniae collected from 3 university hospitals within Korea Get access Jungmin Kim, Kim Department Microbiology, Dankook University College Medicine, San-29, Anseo-dong, Chonan City, Choongnam 330-715, Republic Search for other works by this author on: Oxford Academic PubMed Google Scholar Yu-Mi Lim, Lim *Corresponding author. Tel.: +82 53 420...

Using 58 isolates of Acinetobacter species recovered from a university hospital between August 2004 and March 2005, we performed genomic identification by amplified rRNA gene restriction analysis (ARDRA) investigated the existence metallo-beta-lactamase (MBL) producers extended-spectrum beta-lactamase (ESBL) producers. Genomic strains using ARDRA showed that 40 were 2 (Acinetobacter baumannii), 9 13 sensu Tjernberg Ursing (13TU), 5 phenon 6/ct 13TU, 4 genospecies 3. Among strains, MBL...

ABSTRACT The distribution of conjugative-plasmid-mediated 16S rRNA methylase genes among amikacin-resistant Enterobacteriaceae collected between 1995 and 1998 2001 2006 at a university hospital in South Korea was examined, conjugative plasmids carrying the were characterized by PCR-based replicon typing determination their antimicrobial resistance pattern. Among 7,127 isolates, 463 isolates showed high level to amikacin, 218 transferred amikacin conjugation. armA detected 153 (88 Klebsiella...

ABSTRACT The colonization of Staphylococcus aureus is one the most important aggravating factors atopic dermatitis (AD). Until now, importance S. in AD and a positive correlation between with clinical severity/skin barrier function has been demonstrated. aim this study was to determine whether there are certain clones which colonize skin patients. For purpose, genotype isolated from patients examined by newly‐developed typing methods. With 36 strains 35 AD, spa typing, multi‐locus sequence...

Polyene macrolides such as nystatin A1 and amphotericin B belong to a large family of very valuable antifungal polyketide compounds typically produced by soil actinomycetes. Recently, nystatin-like Pseudonocardia polyene (NPP) has been identified unique disaccharide-containing tetraene macrolide autotrophica. Despite its significantly increased water solubility decreased hemolytic activity, activity remains limited compared with that A1. In this study, we developed NPP B1, novel derivative...