Acinetobacter baumannii is an important opportunistic pathogen responsible for nosocomial infection. Despite considerable clinical and epidemiological data regarding the role of A. in infection, specific virulence factor or pathogenic mechanism this organism has yet to be elucidated. This study investigated molecular apoptosis on infection human laryngeal epithelial HEp-2 cells with examined contribution outer membrane protein 38 (Omp38) ability induce cells. induced through cell surface...

Abstract Background Acinetobacter baumannii is a nosocomial pathogen of increasing importance, but the pathogenic mechanism this microorganism has not been fully explored. This study investigated potential A. to invade epithelial cells and determined role outer membrane protein A (AbOmpA) in interactions with cells. Results invaded by zipper-like mechanism, which associated microfilament- microtubule-dependent uptake mechanisms. Internalized bacteria were located membrane-bound vacuoles....

Antimicrobial resistance and class 1 integrons found in Escherichia coli isolates from humans animals Korea were characterized.E. examined for susceptibility to antimicrobial agents. Integrase genes amplified. Gene cassette regions classes 2 amplified sequenced. Conjugal transfer Southern hybridization performed determine the genetic localization of integrons. The clonal relationship E. carrying an identical array was analysed by PFGE.Commensal highly resistant commonly used agents such as...

We have previously reported that Porphyromonas gingivalis infection of gingival epithelial cells (GEC) requires an exogenous danger signal such as ATP to activate inflammasome and caspase-1, thereby inducing secretion interleukin (IL)-1β. Stimulation with extracellular also stimulates production reactive oxygen species (ROS) in GEC. However, the mechanism by which ROS is generated response ATP, role different purinergic receptors may play activation, still unclear. In this study, we revealed...

Acinetobacter baumannii is an emerging opportunistic pathogen responsible for healthcare-associated infections. The outer membrane protein A of A. (AbOmpA) the most abundant surface that has been associated with apoptosis epithelial cells through mitochondrial targeting. nuclear translocation AbOmpA and subsequent pathology on host were further investigated. directly binds to eukaryotic cells. translocates nucleus by a novel monopartite localization signal (NLS). introduction rAbOmpA into or...

Bacteremia is a common systemic disease caused by Acinetobacter baumannii, an important hospital-acquired pathogen among critically ill patients. The complement system central to innate immune defense against invading bacteria in the blood. present study investigated susceptibility of clinical A. baumannii isolates normal human sera (NHS), and determined resistance mechanism complement-mediated lysis. survival from bacteremic patients was significantly decreased undiluted NHS, but they were...

Journal Article Phylogenetic group distributions, virulence factors and antimicrobial resistance properties of uropathogenic Escherichia coli strains isolated from patients with urinary tract infections in South Korea Get access J.H. Lee, Lee Department Pediatrics Chungnam National University Hospital Daejeon Search for other works by this author on: Oxford Academic Google Scholar B. Subhadra, Subhadra Microbiology College Medicine Y.‐J. Son, Son D.H. Kim, Kim Correspondence Hwa Jung Chul...

Ligation of P2X7 receptors with a 'danger signal', extracellular ATP (eATP), has recently been shown to result in production intracellular reactive-oxygen-species (ROS) macrophages. We show that primary gingival epithelial cells (GECs) produce sustained, robust cellular ROS upon stimulation by eATP. The induction was mediated receptor signalling coupled NADPH-oxidase activation, as determined pharmacological inhibition and RNA interference. Furthermore, Porphyromonas gingivalis, an oral...

Porphyromonas gingivalis, an opportunistic pathogen usurps gingival epithelial cells (GECs) as primary intracellular niche for its colonization in the oral mucosa. However, precise characterization of trafficking and fate P. gingivalis GECs remains incomplete. Therefore, we employed high-resolution three-dimensional-transmission-electron-microscopy to determine subcellular location human upon invasion. Serial sections infected-GECs their tomographic reconstruction depicted...

ABSTRACT Gene cassettes of class 1 integrons in Escherichia coli isolates from urine specimens collected Korea during the last 2 decades were characterized. intI1 was detected 54% isolates, yet gene cassette regions amplified only 43% isolates. intI2 29 (5%) and no intI3 this study. Twenty-one different genes, including genes encoding resistance to antibiotics, an alcohol dehydrogenase ( adhE ), unknown detected. The most commonly found those for aminoglycoside trimethoprim resistance....

The traditional markerless gene deletion technique based on overlap extension PCR has been used for generating deletions in multidrug-resistant Acinetobacter baumannii. However, the method is time-consuming because it requires restriction digestion of products DNA cloning and construction new vectors containing a suitable antibiotic resistance cassette selection A. baumannii merodiploids. Moreover, availability sites recombinant bacteria harboring desired chimeric plasmid are limited, making...

Abstract Mycobacterium abscessus (MAB) is a species of nontuberculous mycobacteria (NTM) and major causative pathogen pulmonary diseases especially in patients with cystic fibrosis. MAB infection notoriously difficult to treat because its intrinsic or inducible resistance most antibiotics. The rough (R) morphotype MAB, lacking cell surface glycopeptidolipids (GPLs), associated more severe persistent than the smooth (S) type; however, mechanisms underlying R type’s virulence relation GPLs...

Acute cognitive impairments termed delirium often occur after inflammatory insults in elderly patients. While previous preclinical studies suggest mitochondria as a target for reducing neuroinflammation and LPS injection, fewer have evaluated the effects of low-grade systemic inflammation aged brain. Thus, to identify significance mitochondrial dysfunction clinically relevant stimulus, we injected old-aged mice (18–20 months) with low-dose lipopolysaccharide (LPS, 0.04 mg/kg). injection...

Antibody-recruiting molecules (ARMs) have emerged as a promising strategy for enhancing immune responses against pathogens and cancer cells. In this study, we developed novel class of antibacterial ARMs utilizing siderophores, small iron-chelating compounds, targeting motifs. Siderophores naturally exhibit high specificity bacterial due to their role in iron acquisition, making them ideal candidates selective targeting. We identified potent ARM, GNP3, comprising MECAM, siderophore mimetic,...

ABSTRACT Outer membrane protein A (OmpA) plays a vital role in the interactions between Acinetobacter baumannii and host cells. Autophagy is defense mechanism that hinders intracellular replication of bacteria, thereby safeguarding cells against microbial infections. While it has been observed A. triggers cellular autophagy, precise its virulence OmpA this process remains uncertain. In study, we investigated effects (AbOmpA) on autophagy explored underlying molecular mechanisms. We found...

ObjectivesTo investigate the differences in antimicrobial susceptibility and resistance mechanisms against imipenem between Acinetobacter baumannii genomic species 13TU.