A5013686175- Adenosine and Purinergic Signaling
- Microbial infections and disease research
- Toxoplasma gondii Research Studies
- Mechanisms of cancer metastasis
- Streptococcal Infections and Treatments
- Peptidase Inhibition and Analysis
- Neuroscience of respiration and sleep
- Oral microbiology and periodontitis research
- Reproductive tract infections research
- Transplantation: Methods and Outcomes
- Vagus Nerve Stimulation Research
- Aquaculture disease management and microbiota
- Veterinary medicine and infectious diseases
- Pancreatic function and diabetes
- Tryptophan and brain disorders
- Heme Oxygenase-1 and Carbon Monoxide
- Animal health and immunology
- Oral Health Pathology and Treatment
- Proteoglycans and glycosaminoglycans research
- Neuropeptides and Animal Physiology
- Effects of Environmental Stressors on Livestock
- Reproductive Physiology in Livestock
- Pneumonia and Respiratory Infections
- MXene and MAX Phase Materials
- Dental Radiography and Imaging
United States Marine Corps
2020
United States Navy
2020
Medical University of South Carolina
2016-2020
University of Florida
2011-2014
University of St Andrews
1990-1992
Andrews University
1990
Ligation of P2X7 receptors with a 'danger signal', extracellular ATP (eATP), has recently been shown to result in production intracellular reactive-oxygen-species (ROS) macrophages. We show that primary gingival epithelial cells (GECs) produce sustained, robust cellular ROS upon stimulation by eATP. The induction was mediated receptor signalling coupled NADPH-oxidase activation, as determined pharmacological inhibition and RNA interference. Furthermore, Porphyromonas gingivalis, an oral...
Porphyromonas gingivalis and Filifactor alocis are fastidious oral pathogens etiological agents associated with chronic periodontitis. Although previous studies showed increased levels of the two obligate anaerobic species in periodontitis patients, methodologies for this knowledge were primarily limited to sampling subgingival plaque, saliva, or gingival crevicular fluid. To evaluate extent which P. F. may invade periodontal tissues, an situ cross-sectional study was comparatively conducted...
Abstract Porphyromonas gingivalis and Filifactor alocis are fastidious anaerobic bacteria strongly associated with chronic forms of periodontitis. Our understanding the growth activities these microorganisms in situ is very limited. Previous studies have shown that copy numbers ribosomal-RNA precursor (pre-rRNA) specific pathogen species relative to genomic-DNA (gDNA) same (P:G ratios) greater actively growing bacterial cells than resting cells. The method, so-called steady-state...
Extracellular signaling during inflammation and chronic diseases involves molecules referred to as 'Danger Signals' (DS), including the small molecule adenosine. We demonstrate that primary gingival epithelial cells (GEC) express a family of G-protein coupled receptors known adenosine receptors, high-affinity A1 A2a low-affinity A2b A3. Treatment Porphyromonas gingivalis-infected GEC with receptor-specific agonist CGS-21680 resulted in elevated intracellular bacterial replication determined...
Ureaplasma urealyticum strains of all serotypes express a specific human immunoglobulin A1 protease that cleaves to produce intact Fab and Fc fragments. The use variety inhibitors suggests the enzyme is serine protease. N-terminal sequencing digestion product showed between proline threonine residues 235 236 in hinge region heavy chain A1.