A5059092326- Gut microbiota and health
- Ion Channels and Receptors
- Metabolomics and Mass Spectrometry Studies
- Ubiquitin and proteasome pathways
- Forensic and Genetic Research
- Cancer-related gene regulation
- Antibiotic Resistance in Bacteria
- Wnt/β-catenin signaling in development and cancer
- Pneumocystis jirovecii pneumonia detection and treatment
- Cancer Genomics and Diagnostics
- Molecular Biology Techniques and Applications
- Metabolism, Diabetes, and Cancer
- Caveolin-1 and cellular processes
- Probiotics and Fermented Foods
- Pneumonia and Respiratory Infections
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Environmental DNA in Biodiversity Studies
- Autophagy in Disease and Therapy
- Protein Kinase Regulation and GTPase Signaling
- Mass Spectrometry Techniques and Applications
- Bacteriophages and microbial interactions
- Phytochemicals and Antioxidant Activities
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Forensic Entomology and Diptera Studies
Eulji University
2014-2024
Kyungpook National University
2005
Samsung (South Korea)
2004
Acinetobacter baumannii is an important opportunistic pathogen responsible for nosocomial infection. Despite considerable clinical and epidemiological data regarding the role of A. in infection, specific virulence factor or pathogenic mechanism this organism has yet to be elucidated. This study investigated molecular apoptosis on infection human laryngeal epithelial HEp-2 cells with examined contribution outer membrane protein 38 (Omp38) ability induce cells. induced through cell surface...
Acinetobacter baumannii is an emerging opportunistic pathogen responsible for healthcare-associated infections. The outer membrane protein A of A. (AbOmpA) the most abundant surface that has been associated with apoptosis epithelial cells through mitochondrial targeting. nuclear translocation AbOmpA and subsequent pathology on host were further investigated. directly binds to eukaryotic cells. translocates nucleus by a novel monopartite localization signal (NLS). introduction rAbOmpA into or...
Outer membrane vesicles (OMVs) derived from pathogenic Gram-negative bacteria are an important vehicle for delivery of effector molecules to host cells, but the production OMVs Klebsiella pneumoniae, opportunistic pathogen both nosocomial and community-acquired infections, their role in bacterial pathogenesis have not yet been determined. In present study, we examined K. pneumoniae determined induction innate immune response against OMVs. ATCC 13883 produced secreted during vitro culture....
(1) Background: Hepatocellular carcinoma (HCC) is one of the leading causes cancer-related death worldwide. Although various serum enzymes have been utilized for diagnosis and prognosis HCC, currently available biomarkers lack sensitivity needed to detect HCC at early stages accurately predict treatment responses. (2) Methods: We our highly sensitive cell-free DNA (cfDNA) detection system, in combination with a machine learning algorithm, provide platform improved HCC. (3) Results: cfDNA,...
Synbiotics intake can alter the composition of intestinal microbes beneficially. We aimed to detect changes in microbiomes 37 healthy elderly Korean women after a synbiotic drink. This was longitudinal study controlled with temporal series, including control period 3 weeks before intake, for weeks, and washout weeks. Fecal microbiota analyzed by sequencing V3-V4 hypervariable regions 16S rRNA. Physical fecal activity increased improvement shape. Thirty bacterial taxa were observed change...
Introduction Identification of key microbiome components has been suggested to help address the maintenance oral and intestinal health in humans. The core is similar all individuals, whereas diverse varies across based on their unique lifestyles phenotypic genotypic determinants. In this study, we aimed predict metabolism microorganisms gut environment enterotyping orotyping. Materials methods Gut samples were collected from 83 Korean women aged 50 years or older. extracted DNA was subjected...
Bloodstains are common evidence in crime scenes, containing significant information, including genetic information. Although efforts have been made to reliably determine the time of incident by analyzing elapsed bloodstain, there has limited success. To identify candidate metabolites bloodstains over time, we prepared bloodstain samples using filter paper and analyzed high-performance liquid chromatography–mass spectrometry (HPLC-MS)/MS a 21-day period. Using Venn diagrams multivariate...
The TRPV4 cation channel, a member of the TRP vanilloid subfamily, is expressed in broad range tissues where it participates generation Ca2+ signal and/or depolarization membrane potential. Here, we identified stromal interaction molecule 1 precursor (STIM1) as an auxiliary protein this epithelial Ca2+channel using confocal microscopy analysis and GST pull-down assay. STIM1 associates specifically with C-terminal tail to form complex. In previous reports, demonstrated that serine824 residue...
The outer membrane protein A of Acinetobacter baumannii (AbOmpA) is an important pathogen-associated molecular pattern that induces host cell death. We determined the gene expression profiles human laryngeal epithelial HEp-2 cells in response to sublethal concentration recombinant AbOmpA (rAbOmpA) and investigated mechanisms by which rAbOmpA innate immune response. microarray analysis showed sequentially regulated a relatively small set genes, including those associated with signal...
Modification of the Small Ubiquitin-like Modifier (SUMO) (SUMOylation) appears to regulate diverse cellular processes, including nuclear transport, signal transduction, apoptosis, autophagy, cell cycle control, ubiquitin-dependent degradation and gene transcription. Glycogen synthase kinase 3β (GSK 3β) is a serine/threonine that thought contribute variety biological events, embryonic development, metabolism, tumorigenesis, death. GSK constitutively active regulates many intracellular...
Acinetobacter baumannii outer membrane protein A (AbOmpA) is a potential virulence factor that induces host cell death. Based on previous findings AbOmpA translocated into the nuclei of cells, cell-death mechanism through nuclear targeting was investigated. secreted in vitro culture. The recombinant (rAbOmpA) internalized by cells. intracellular rAbOmpA degraded several forms subfragments cytosol and then two nuclei. exhibited divalent cation-dependent endonuclease activity. In an vivo assay...
Glycogen synthase kinase-3β(GSK-3β), which is a member of the serine/threonine kinase family, has been shown to be crucial for cellular survival, differentiation, and metabolism. Here, we present evidence that GSK-3β associated with karyopherin β2 (Kap β2) (102-kDa), functions as substrate transportation into nucleus. A potential PY-NLS motif (109IVRLRYFFY117) was observed, similar consensus PY NLS (R/K/H)X 2–5PY in catalytic domain. Using pull down approach, observed physically interacts...
Abstract With the consensus sequence information of serum glucocorticoid-induced protein kinase-1 (SGK1) phosphorylation site {R-X-R-X-X-(S/T)Φ; where Φ is any hydrophobic amino acid}, we noticed that transient receptor potential vanilloid 4 (TRPV4) cation channel, a member TRP subfamily, harbors putative SGK1 (on its Ser 824). We have demonstrated TRPV4 an authentic substrate protein, with on 824 by SGK1. Further, using mutants (S824A and S824D), noted modification activates Ca2 + entry,...
Uncoordinated 51-like kinase 2 (ULK2), a member of the serine/threonine family, plays an essential role in regulation autophagy mammalian cells. Given normal cellular homeostasis and multiple diseases, improved mechanistic insight into this process may result development novel therapeutic approaches. Here, we present evidence that ULK2 associates with karyopherin beta (Kapβ2) for its transportation nucleus. We identify potential PY-NLS motif (774gpgfgssppGaeaapslRyvPY795) S/P space domain...
The transient receptor potential vanilloid 4 (TRPV4) cation channel, a member of the TRP subfamily, is expressed in broad range tissues. Nitric oxide (NO) as gaseous signal mediator shows variety important biological effects. In many instances, NO has been shown to exhibit its activities via protein S-nitrosylation mechanism order regulate functions. With functional assays site-directed mutagenesis, we demonstrate herein that induces TRPV4 Ca(2+) channel on Cys(853) residue, and reduced...
Fermented red ginseng (FRG) has been used as a general stimulant and herbal medicine for health promotion in Asia thousands of years. Few studies have investigated the effects FRG containing prebiotics on gut microbiota. Here, 29 Korean women aged ≥ 50 years were administered three weeks to determine its effect stool characteristics, biochemical parameters, microbiome. Gut microbial DNA was subjected 16S rRNA V3-V4 region sequencing assess distribution different stages. Additionally,...
Fe65 is characterized as an adaptor precursor (APP) through its PID2 element, well with the other members of APP protein family. With serum- and glucocorticoid-induced kinase 1 (SGK1) substrate specificity information, we found that putative site phosphorylation in by SGK1 present on Ser(566) residue (560)CRVRFLSFLA(569)(X60469). Thus, demonstrated fluorescein-labeled peptide FITC-(560)CRVRFLSFLA(569) are phosphorylated vitro SGK1. Phosphorylation was also using a Ser566 phospho-specific...
Probiotics can be used as a nutritional strategy to improve gut homeostasis. We aimed evaluate the intestinal microbiota profile of 18 subjects after ingestion probiotic yogurt powder (PYP) based on enterotype. The were classified into three enterotypes according their microbial community: Bacteroides (n = 9, type B), Prevotella 3, P), and Ruminococcus 6, R). performed controlled termination in transient series that included control period weeks before intake, PYP intake for weeks,...