A5054578884- Ubiquitin and proteasome pathways
- Ion Channels and Receptors
- Protein Kinase Regulation and GTPase Signaling
- Autophagy in Disease and Therapy
- Endoplasmic Reticulum Stress and Disease
- 14-3-3 protein interactions
- Metabolism, Diabetes, and Cancer
- Wnt/β-catenin signaling in development and cancer
- Pneumocystis jirovecii pneumonia detection and treatment
- Cancer-related gene regulation
- Ion channel regulation and function
- Cell death mechanisms and regulation
- Environmental DNA in Biodiversity Studies
- Cell Adhesion Molecules Research
- RNA and protein synthesis mechanisms
- Pancreatic function and diabetes
- RNA Research and Splicing
- Pneumonia and Respiratory Infections
- Nitric Oxide and Endothelin Effects
- PI3K/AKT/mTOR signaling in cancer
- Phytochemicals and Antioxidant Activities
- Signaling Pathways in Disease
- Genetics and Neurodevelopmental Disorders
- FOXO transcription factor regulation
- Caveolin-1 and cellular processes
Chungbuk National University
2013-2024
Gyeongsang National University
2020
Cheongju National University of Education
2001-2019
Myongji University
2004
Seoul National University of Education
1997
With the amino acid sequences of all reported Akt kinase physiological substrates, possible substrate specificity has been suggested. The serine/threonine residue to be phosphorylated in these proteins is placed within stretches acids with homology, and arginine residues on -5 -3 positions a hydrophobic +2 position are conserved relative those (XXRXRXXS/TXX). We noticed two putative phosphorylation sites (220GARRRGGSAS229) (817AVRIRGKSYV826) human telomerase reverse transcriptase (hTERT)...
A putative Akt kinase phosphorylation site (<sup>64</sup>yd<i>R</i>I<i>R</i>plSYp<sup>73</sup>) was found in Rac1/CDC42 and Rho family proteins (RhoA, RhoB, RhoC, RhoG). Phosphorylation of Rac1 by assayed with recombinant protein the fluorescein-labeled peptide. It shown that peptide were phosphorylated activated lysate SK-MEL28 cells, a human melanoma cell line. The inhibited its GTP-binding activity without any significant change GTPase activity. Both activities S71A (with serine residue...
The protein serine-threonine kinase Akt mediates cell survival signaling initiated by various growth-promoting factors such as insulin. Here we report that SEK1 is a target of in intact cells. Insulin inhibited the anisomycin-induced stimulation both endogenous and its substrate c-Jun N-terminal (JNK), but not upstream MEKK1, 293T inhibitory action insulin on or JNK1 activation was prevented phosphatidylinositol 3-kinase inhibitor LY294002. Expression constitutively active form also...
Outer membrane vesicles (OMVs) derived from pathogenic Gram-negative bacteria are an important vehicle for delivery of effector molecules to host cells, but the production OMVs Klebsiella pneumoniae, opportunistic pathogen both nosocomial and community-acquired infections, their role in bacterial pathogenesis have not yet been determined. In present study, we examined K. pneumoniae determined induction innate immune response against OMVs. ATCC 13883 produced secreted during vitro culture....
Abstract We investigated whether the snake venom toxin (SVT) from Vipera lebetina turanica inhibits cell growth of human prostate cancer cells by inducing apoptosis and also studied possible signaling pathways involved in this death. SVT inhibited PC-3 DU145 cells, androgen-independent but not LNCaP a androgen-dependent cell. Cells were arrested G2-M phase with concomitant decrease expression regulatory protein cyclin B1 G1-S decreasing cyclin-dependent kinase 4, D1 E. In addition to...
Abstract BACKGROUND With an increasing aging population, postmenopausal osteoporosis has become a global public health problem. Previous evidence shown that is skeletal disease mainly caused by estrogen deficiency, generally accompanied inflammation, and dietary isoflavones may ameliorate anti‐inflammatory activity. We have generated isoflavone‐enriched soybean leaves (IESLs), but their activity effect on attenuating are still obscure. Here, we determined the isoflavone profiles of IESLs...
The microtubule-associated protein, tau, is involved in numerous neuronal processes such as vesicle transport, microtubule-plasma membrane interaction and the intracellular localization of proteins. Tau known to be phosphorylated by several kinases mitogen activated protein kinase, microtubule affinity regulating kinase A. We found a putative serum- glucocorticoid-induced 1 (SGK1) phosphorylation site within 207GSRSRTPSLP216 tau amino acid sequence. report here that SGK1 phosphorylates...
Synbiotics intake can alter the composition of intestinal microbes beneficially. We aimed to detect changes in microbiomes 37 healthy elderly Korean women after a synbiotic drink. This was longitudinal study controlled with temporal series, including control period 3 weeks before intake, for weeks, and washout weeks. Fecal microbiota analyzed by sequencing V3-V4 hypervariable regions 16S rRNA. Physical fecal activity increased improvement shape. Thirty bacterial taxa were observed change...
The present study was undertaken to determine how tumour necrosis factor-α (TNF-α) elicits the inhibition of glucose-stimulated insulin secretion (GSIS) in rat insulinoma cells (INS)-1 β-cells. TNF-α pretreatment did not change expression levels insulin, PDX-1, glucose transporter 2, glucokinase, K ATP channels, Ca 2 + and exocytotic molecules and, furthermore, reduce level. On other hand, reduced influx . treatment thought activate c-Jun N-terminal kinase (JNK), p38 mitogen-activated...
The TRPV4 cation channel, a member of the TRP vanilloid subfamily, is expressed in broad range tissues where it participates generation Ca2+ signal and/or depolarization membrane potential. Here, we identified stromal interaction molecule 1 precursor (STIM1) as an auxiliary protein this epithelial Ca2+channel using confocal microscopy analysis and GST pull-down assay. STIM1 associates specifically with C-terminal tail to form complex. In previous reports, demonstrated that serine824 residue...
Diverse stimuli initiate the activation of apoptotic signaling pathways that often causes nuclear DNA fragmentation. Here, we report a new antiapoptotic protein, caspase-activated DNase (CAD) inhibitor interacts with ASK1 (CIIA). CIIA, by binding to apoptosis signal-regulating kinase 1 (ASK1), inhibits oligomerization-induced activation. CIIA also associates CAD and nuclease activity without affecting caspase-3–mediated ICAD cleavage. Overexpressed reduces H2O2- tumor necrosis...
Modification of the Small Ubiquitin-like Modifier (SUMO) (SUMOylation) appears to regulate diverse cellular processes, including nuclear transport, signal transduction, apoptosis, autophagy, cell cycle control, ubiquitin-dependent degradation and gene transcription. Glycogen synthase kinase 3β (GSK 3β) is a serine/threonine that thought contribute variety biological events, embryonic development, metabolism, tumorigenesis, death. GSK constitutively active regulates many intracellular...
Glycogen synthase kinase-3β(GSK-3β), which is a member of the serine/threonine kinase family, has been shown to be crucial for cellular survival, differentiation, and metabolism. Here, we present evidence that GSK-3β associated with karyopherin β2 (Kap β2) (102-kDa), functions as substrate transportation into nucleus. A potential PY-NLS motif (109IVRLRYFFY117) was observed, similar consensus PY NLS (R/K/H)X 2–5PY in catalytic domain. Using pull down approach, observed physically interacts...
Abstract With the consensus sequence information of serum glucocorticoid-induced protein kinase-1 (SGK1) phosphorylation site {R-X-R-X-X-(S/T)Φ; where Φ is any hydrophobic amino acid}, we noticed that transient receptor potential vanilloid 4 (TRPV4) cation channel, a member TRP subfamily, harbors putative SGK1 (on its Ser 824). We have demonstrated TRPV4 an authentic substrate protein, with on 824 by SGK1. Further, using mutants (S824A and S824D), noted modification activates Ca2 + entry,...