A5010624311- Pancreatic function and diabetes
- Antibiotic Resistance in Bacteria
- Autophagy in Disease and Therapy
- Metabolism, Diabetes, and Cancer
- Enterobacteriaceae and Cronobacter Research
- Pharmaceutical and Antibiotic Environmental Impacts
- Microbial Metabolites in Food Biotechnology
- GDF15 and Related Biomarkers
- Diet, Metabolism, and Disease
- Gut microbiota and health
- Regulation of Appetite and Obesity
- Biochemical Analysis and Sensing Techniques
- Health and Wellbeing Research
- Fungal Biology and Applications
- Cannabis and Cannabinoid Research
- Education and Learning Interventions
- Food composition and properties
- Enzyme Production and Characterization
- Helicobacter pylori-related gastroenterology studies
- Sphingolipid Metabolism and Signaling
- Educational Research and Pedagogy
- Endoplasmic Reticulum Stress and Disease
- Natural product bioactivities and synthesis
- Nuclear Receptors and Signaling
- Education, Safety, and Science Studies
Kangbuk Samsung Hospital
2020-2024
Sungkyunkwan University
2010-2024
Toronto General Hospital
2020-2024
Toronto General Hospital Research Institute
2020-2023
National Development Institute of Korean Medicine
2017-2022
Yonsei University
2016-2021
Korea Health Promotion Institute
2013
Korea Naval Academy
2013
Kyungpook National University
2005-2013
Samsung Medical Center
2010-2012
Islet amyloid accumulation is a hallmark of human type 2 diabetes (T2D). In contrast to islet polypeptide (hIAPP), murine (mIAPP) does not exhibit amyloidogenic propensity. Because autophagy important in the clearance amyloid-like proteins, we studied transgenic mice with β cell–specific expression hIAPP evaluate contribution T2D-associated hIAPP. hIAPP, deficiency resulted development overt diabetes, which was observed expressing alone or lacking alone. Furthermore, lack hIAPP-expressing...
The mechanism of FFA-induced insulin resistance is not fully understood. We have searched for effector molecules(s) in resistance. Palmitic acid (PA) but oleic (OA) induced L6 myotubes through C-Jun N-terminal kinase (JNK) and receptor substrate 1 (IRS-1) Ser307 phosphorylation. Inhibitors ceramide synthesis did block by PA. However, inhibition the conversion PA to lysophosphatidylcholine (LPC) calcium-independent phospholipase A₂ (iPLA₂) inhibitors, such as bromoenol lactone (BEL) or...
The nonobese diabetic (NOD) mouse is a classical animal model for autoimmune type 1 diabetes (T1D), closely mimicking features of human T1D. Thus, the NOD presents an opportunity to test effectiveness induced pluripotent stem cells (iPSCs) as therapeutic modality Here, we demonstrate proof concept cellular therapy using mouse-derived iPSCs (NOD-iPSCs). We generated from embryonic fibroblasts or pancreas-derived epithelial (NPEs), and applied directed differentiation protocols differentiate...
Among 603 isolates of Enterobacteriaceae collected between June and November 2003 from three university hospitals within Korea, bla(CTX-M-3), bla(CTX-M-15), bla(CTX-M-14), bla(CTX-M-9) were detected in 41 species five different genera Enterobacteriaceae, Escherichia coli, Klebsiella pneumoniae, Citrobacter freundii, Enterobacter spp., Serratia marcescens.
Secretagogin (SCGN), a Ca(2+)-binding protein having six EF-hands, is selectively expressed in pancreatic β-cells and neuroendocrine cells. Previous studies suggested that SCGN enhances insulin secretion by functioning as Ca(2+)-sensor protein, but the underlying mechanism has not been elucidated. The present study explored which glucose-induced NIT-1 insulinoma To determine whether influences first or second phase of secretion, we examined how affects kinetics We found silencing suppressed...
Conjugated bile acids are metabolised by upper small intestinal microbiota, and serum levels of taurine-conjugated elevated correlated with insulin resistance in people type 2 diabetes. However, whether changes necessary for microbiome to alter action remain unknown.We evaluated circulating specifically brain using the pancreatic-euglycaemic clamps high-fat (HF) versus chow fed rats or without healthy transplant. Chemical molecular gain/loss-of-function experiments targeting specific...
Journal Article CTX-M and SHV-12 β-lactamases are the most common extended-spectrum enzymes in clinical isolates of Escherichia coli Klebsiella pneumoniae collected from 3 university hospitals within Korea Get access Jungmin Kim, Kim Department Microbiology, Dankook University College Medicine, San-29, Anseo-dong, Chonan City, Choongnam 330-715, Republic Search for other works by this author on: Oxford Academic PubMed Google Scholar Yu-Mi Lim, Lim *Corresponding author. Tel.: +82 53 420...
The resistance mechanism of extended-spectrum cephalosporins in clinical isolates Citrobacter freundii, Enterobacter spp., and Serratia marcescens was studied. Of 152 isolates, 45 (29.6%) were derepressed AmpC mutants 39 (25.7%) produced beta-lactamase (ESBLs). most prevalent ESBLs CTX-M enzymes, followed by TEM-52 SHV-12.
Sodium-dependent glucose cotransporter 2 (SGLT2) mediates reabsorption in the renal proximal tubules, and SGLT2 inhibitors are used as therapeutic agents for treating type diabetes mellitus. This study aimed to elucidate effects mechanisms of inhibition on hepatic metabolism both serum deprivation supplementation states.
The mechanism of nutrient sensing in the upper small intestine (USI) and ileum that regulates glucose homeostasis remains elusive. Short-term high-fat (HF) feeding increases taurochenodeoxycholic acid (TCDCA; an agonist farnesoid X receptor (FXR)) USI rats, increase TCDCA is prevented by transplantation microbiota obtained from healthy donors into HF rats. However, whether changes TCDCA-FXR axis alter unknown.Intravenous tolerance test was performed rats received or ileal infusion nutrients...
Orostachys japonicus A. Berger (), known as Wa-song in Korea, has been reported to exert various biological effects, such anti-tumor, anti-oxidant, and anti-febrile effects. However, the anti-angiogenic effects of O.japonicus extracts remain be investigated. In present study, we demonstrated bioconverted O. extract (BOE) Ms-1 mouse endothelial cells compared them with bioactivities (OE). BOE, but not OE, were found including inhibition cell migration, adhesion, tube formation cells, blood...
Akebia quinata, commonly called chocolate vine, has various bioactivities, including antioxidant and anti-obesity properties. However, the effects of bioconverted extracts A. quinate have not been examined. In this study, quinata fruit was using enzyme isolated from soybean paste fungi Aspergillus kawachii. To determine whether bioconversion process could influence extracts, we employed 3T3-L1 adipocytes HFD-induced obese rats. We observed that extract (BFE) afforded effects, which were...
Hypothalamic regulation of lipid and glucose homeostasis is emerging, but whether the dorsal vagal complex (DVC) senses nutrients regulates hepatic nutrient metabolism remains unclear. Here, we found in rats DVC oleic acid infusion suppressed secretion triglyceride-rich very-low-density lipoprotein (VLDL-TG), which was disrupted by inhibiting long-chain fatty acyl-CoA synthetase that parallel disturbed during intravenous infusion. elevated local levels similarly as production. This...
Mitogen-activated protein (MAP) kinases play an important role in cell growth and differentiation, as well the modulation of proinflammatory cytokines. The objective this study was to examine increase anti-inflammatory effect Gentiana scabra Bunge (GSB), due bioconversion with Aspergillus kawachii crude enzyme, via inhibition NF-κB signaling MAP kinase pathways RAW 264.7 cells. expression inducible nitric oxide synthase (iNOS) cyclooxygenase-2 cells treated GSB ethyl acetate fraction...
Acinetobacter species are non-fermentative Gram-negative coccobacilli and they have emerged as important nosocomial pathogens which associated with the significant multidrug resistance in recent years. Carbapenem-resistant A. baumannii (CRAB) pandrug-resistant (PDRAB) were reported 1991 1998, respectively. Fifty-eight isolates of recovered from a university hospital between August 2004 March 2005 investigated for existence CRAB, PDRAB, extended-spectrum β-lactamase (ESBL)-producing examined...
Gamma-aminobutyric acid (GABA) aminotransferase (gabT) and succinic semialdehyde dehydrogenase (gabD) genes from Pseudomonas fluorescens KCCM 12537 were cloned into a single pETDuet-1 vector co-expressed in Escherichia coli BL21(DE3) simultaneously. The mixture of both enzymes, called GABase, is the key enzyme for enzymatic analysis GABA. molecular mass GABA determined to be 52.8 46.7 kDa following computations performed with pI/Mw program, respectively. GABase activity between pH 6.0 9.0 24...
The ethyl acetate fraction of Bat Faeces (Ye Ming Sha: natural products used in Chinese Medicine) after fermentation (EFBF-AF) showed enhanced anti-oxidative effects 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt assays. Fermentation the by using crude enzyme extract from Aspergillus kawachii, significantly increased anti-inflammatory effects. Fermented markedly inhibited nitric oxide production, inducible synthase, cyclooxygenase-2...