A5105769717- Algal biology and biofuel production
- Photosynthetic Processes and Mechanisms
- Lipid metabolism and biosynthesis
- Plant Surface Properties and Treatments
- Marine and coastal ecosystems
- Plant biochemistry and biosynthesis
- Soil Carbon and Nitrogen Dynamics
- Plant Gene Expression Analysis
- Plant and Fungal Species Descriptions
- Postharvest Quality and Shelf Life Management
- Microbial Metabolic Engineering and Bioproduction
- Plant Molecular Biology Research
- Genomics and Phylogenetic Studies
- Horticultural and Viticultural Research
- Plant Parasitism and Resistance
- Genetic diversity and population structure
- Light effects on plants
- Plant nutrient uptake and metabolism
- Plant Reproductive Biology
- Plant Stress Responses and Tolerance
- Plant Pathogens and Fungal Diseases
- Enzyme Catalysis and Immobilization
- Berry genetics and cultivation research
- Aquatic Ecosystems and Phytoplankton Dynamics
- Plant responses to water stress
Institut de Biosciences et Biotechnologies
2016-2025
Aix-Marseille Université
2016-2025
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2016-2025
Centre National de la Recherche Scientifique
2016-2025
Institut de Biosciences et biotechnologies d'Aix-Marseille
2019-2025
CEA Cadarache
2016-2025
Henan Agricultural University
2014-2024
China Agricultural University
2013-2024
Guangxi University of Chinese Medicine
2017-2023
China North Industries Group Corporation (China)
2020-2023
Acyl lipids in Arabidopsis and all other plants have a myriad of diverse functions. These include providing the core diffusion barrier membranes that separates cells subcellular organelles. This function alone involves more than 10 membrane lipid classes, including phospholipids, galactolipids, sphingolipids, within each class variations acyl chain composition expand number structures to several hundred possible molecular species. form triacylglycerol account for 35% weight seeds represent...
When cultivated under stress conditions, many microalgae species accumulate both starch and oil (triacylglycerols). The model green microalga Chlamydomonas reinhardtii has recently emerged as a to test genetic engineering or cultivation strategies aiming at increasing lipid yields for biodiesel production. Blocking synthesis been suggested way boost accumulation. Here, we characterize the triacylglycerol (TAG) accumulation process in quantify TAGs various wild-type starchless strains.In...
Abstract Suberin and cutin are fatty acid– glycerol-based plant polymers that act as pathogen barriers function in the control of water solute transport. However, despite important physiological roles, their biosynthetic pathways, including acyl transfer reactions, remain hypothetical. We report characterization two suberin mutants (gpat5-1 gpat5-2) Arabidopsis thaliana GPAT5, encoding a protein with acyl-CoA:glycerol-3-phosphate acyltransferase activity. RT-PCR β-glucuronidase–promoter...
Algal enzyme driven by blue light Microalgae make hydrocarbons. In searching for the responsible, Sorigué et al. found a glucose-methanolcholine oxidoreductase (see Perspective Scrutton). Expression of in Escherichia coli showed that hydrocarbon production requires visible light. fact, constant input photons to carry out its catalytic reaction. A long hydrophobic tunnel stabilizes fatty acid substrates proximity flavin adenine dinucleotide cofactor. Science , this issue p. 903 ; see also 872
Cutin and suberin are the two major lipid-based polymers of plants. is structural polymer epidermal cuticle, waterproof layer covering primary aerial organs which often structure first encountered by phytopathogens. Suberin contributes to control diffusion water solutes across internal root tissues in periderms. The enzymes responsible for assembly cutin largely unknown. We have identified Arabidopsis acyltransferases essential biosynthesis, glycerol-3-phosphate acyltransferase (GPAT) 4...
Acyl lipids in Arabidopsis and all other plants have a myriad of diverse functions. These include providing the core diffusion barrier membranes that separates cells subcellular organelles. This function alone involves more than 10 membrane lipid classes, including phospholipids, galactolipids, sphingolipids, within each class variations acyl chain composition expand number structures to several hundred possible molecular species. form triacylglycerol account for 35% weight seeds represent...
Plant neighbor detection and response strategies are important mediators of interactions among species. Despite increasing knowledge involving plant volatiles, less is known about how soil-borne signaling chemicals may act belowground in plant-plant interactions. Here, we experimentally demonstrate allelopathic responses between wheat 100 other species via signaling. Wheat can detect both conspecific heterospecific neighbors responds by allelochemical production. Furthermore, show that...
Light makes light work of fatty acids Photosynthetic organisms are notable for their ability to capture energy and use it power biosynthesis. Some algae have gone a step beyond photosynthesis can initiate enzymatic photodecarboxylation acids, producing long-chain hydrocarbons. To understand this transformation, Sorigué et al. brought bear an array structural, computational, spectroscopic techniques fully characterized the catalytic cycle enzyme. These experiments consistent with mechanism...
Distinctive nanoridges on the surface of flowers have puzzled plant biologists ever since their discovery over 75 years ago. Although postulated to help attract insect pollinators, function, chemical nature, and ontogeny these nanostructures remain uncertain. Studies been hampered by fact that no ridgeless mutants identified. Here, we describe two lacking define biosynthetic pathway for 10,16-dihydroxypalmitate, a major cutin monomer in nature. Using gene expression profiling, candidates...
Abstract All plants produce suberin, a lipophilic barrier of the cell wall that controls water and solute fluxes restricts pathogen infection. It is often described as heteropolymer comprised polyaliphatic polyaromatic domains. Major monomers include ω-hydroxy α,ω-dicarboxylic fatty acids, glycerol, ferulate. No genes have yet been identified for aromatic suberin pathway. Here we demonstrate Arabidopsis (Arabidopsis thaliana) gene AT5G41040, member BAHD family acyltransferases, essential...
Abstract Oil bodies are sites of energy and carbon storage in many organisms including microalgae. As a step toward deciphering oil accumulation mechanisms algae, we used proteomics to analyze purified from the model microalga Chlamydomonas reinhardtii grown under nitrogen deprivation. Among 248 proteins (≥2 peptides) identified by LC‐MS/MS, 33 were putatively involved metabolism lipids (mostly acyl‐lipids sterols). Compared with recently reported body proteome, 19 new lipid identified,...
Abstract Arabidopsis (Arabidopsis thaliana) has eight glycerol-3-phosphate acyltransferase (GPAT) genes that are members of a plant-specific family with three distinct clades. Several these GPATs required for the synthesis cutin or suberin. Unlike sn-1 regiospecificity involved in membrane storage lipid synthesis, GPAT4 and -6 unique bifunctional enzymes both sn-2 phosphatase activity resulting 2-monoacylglycerol products. We present enzymology, pathway organization, evolutionary analysis...
The first step in assembly of membrane and storage glycerolipids is acylation glycerol-3-phosphate (G3P). All previously characterized membrane-bound, eukaryotic G3P acyltransferases (GPATs) acylate the sn -1 position to produce lysophosphatidic acid (1-acyl-LPA). Cutin a glycerolipid with omega-oxidized fatty acids glycerol as integral components. It occurs an extracellular polyester on aerial surface all plants, provides barrier pathogens resistance stress, maintains organ identity. We...
Studying how photosynthetic cells modify membrane lipids in response to heat stress is important understand plants and microalgae adapt daily fluctuations temperature investigate new lipid pathways. Here, we changes occurring molecular species metabolism genes during early the model microorganism Chlamydomonas reinhardtii. Lipid analyses revealed that, after 60 min at 42 °C, a strong decrease specific polyunsaturated was observed together with an increase triacylglycerols (TAGs)...