A5047725986- Algal biology and biofuel production
- Enzyme Catalysis and Immobilization
- Photosynthetic Processes and Mechanisms
- Microbial Metabolic Engineering and Bioproduction
- Radical Photochemical Reactions
- Light effects on plants
- Photoreceptor and optogenetics research
- Metabolomics and Mass Spectrometry Studies
- Photochemistry and Electron Transfer Studies
- bioluminescence and chemiluminescence research
- Marine and coastal ecosystems
- Lipid metabolism and biosynthesis
- Cancer, Hypoxia, and Metabolism
- Analytical Chemistry and Sensors
- Mass Spectrometry Techniques and Applications
- Click Chemistry and Applications
- Microfluidic and Capillary Electrophoresis Applications
CEA Cadarache
2016-2024
Aix-Marseille Université
2016-2024
Centre National de la Recherche Scientifique
2016-2024
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2016-2024
Institut de Biosciences et Biotechnologies
2016-2024
Princeton University
2024
Cornell University
2023
Algal enzyme driven by blue light Microalgae make hydrocarbons. In searching for the responsible, Sorigué et al. found a glucose-methanolcholine oxidoreductase (see Perspective Scrutton). Expression of in Escherichia coli showed that hydrocarbon production requires visible light. fact, constant input photons to carry out its catalytic reaction. A long hydrophobic tunnel stabilizes fatty acid substrates proximity flavin adenine dinucleotide cofactor. Science , this issue p. 903 ; see also 872
Light makes light work of fatty acids Photosynthetic organisms are notable for their ability to capture energy and use it power biosynthesis. Some algae have gone a step beyond photosynthesis can initiate enzymatic photodecarboxylation acids, producing long-chain hydrocarbons. To understand this transformation, Sorigué et al. brought bear an array structural, computational, spectroscopic techniques fully characterized the catalytic cycle enzyme. These experiments consistent with mechanism...
Microalgae are considered a promising platform for the production of lipid-based biofuels. While oil accumulation pathways intensively researched, possible existence microalgal converting fatty acids into alka(e)nes has received little attention. Here, we provide evidence that such pathway occurs in several species from green and red lineages. In Chlamydomonas reinhardtii (Chlorophyceae), C17 alkene, n-heptadecene, was detected cell pellet headspace liquid cultures. The alkene identified as...
Fatty acid photodecarboxylase (FAP) is one of the few enzymes that require light for their catalytic cycle (photoenzymes). FAP was first identified in microalga Chlorella variabilis NC64A, and belongs to an algae-specific subgroup glucose-methanol-choline oxidoreductase family. While from C. its Chlamydomonas reinhardtii homolog CrFAP have demonstrated vitro activities, activities physiological functions not been studied vivo. Furthermore, conservation activity beyond green microalgae...
Ongoing climate change is driving the search for renewable and carbon-neutral alternatives to fossil fuels. Photocatalytic conversion of fatty acids hydrocarbons by acid photodecarboxylase (FAP) represents a promising route green However, alleged low activity FAP on C2 C12 seemed preclude use synthesis gasoline-range hydrocarbons. Here, we reveal that Chlorella variabilis ( Cv FAP) can convert n -octanoic in vitro four times faster than -hexadecanoic acid, its best substrate reported date....
Abstract Use of microbes to produce liquid transportation fuels is not yet economically viable. A key point reduce production costs the design a cell factory that combines continuous drop-in fuel molecules with ability recover products from culture at low cost. Medium-chain hydrocarbons seem ideal targets because they can be produced abundant fatty acids and, due their volatility, easily collected in gas phase. However, pathways used require two steps, efficient enzymes and/or complex...
In fatty acid photodecarboxylase (FAP), light-induced formation of the primary radical product RCOO⋅ from RCOO
Fatty acid photodecarboxylase (FAP), one of the few natural photoenzymes characterized so far, is a promising biocatalyst for lipid-to-hydrocarbon conversion using light. However, optimum supramolecular organization under which fatty (FA) substrate should be presented to FAP has not been addressed. Using palmitic embedded in phospholipid liposomes, phospholipid-stabilized microemulsions, and mixed micelles, we show that displays preference FAs present liposomes at surface microemulsions. The...
ABSTRACT Fatty acid photodecarboxylase (FAP) is one of the three enzymes that require light for their catalytic cycle (photoenzymes). FAP has been first identified in green microalga Chlorella variabilis NC64A and belongs an algae-specific subgroup glucose-methanol-choline oxidoreductase family. While from its Chlamydomonas reinhardtii homolog CrFAP have demonstrated vitro activity, activity physiological function not studied vivo . Besides, conservation beyond microalgae remains...
Abstract In fatty acid photodecarboxylase (FAP), light‐induced formation of the primary radical product RCOO⋅ from RCOO − occurs in 300 ps, upon which CO 2 is released quasi‐immediately. Based on hypothesis that aliphatic (spectroscopically uncharacterized because unstable) absorbs red similarly to aromatic carbonyloxy radicals such as 2,6‐dichlorobenzoyloxy (DCB⋅), much longer‐lived linear has been suggested recently. We performed quantum chemical reaction pathway and spectral calculations....
Abstract Fatty Acid Photodecarboxylase (FAP) has emerged as a promising catalyst for the biological production of long-chain hydrocarbons. We have recently shown that purified FAP or FAP-expressing bacteria can efficiently convert octanoic acid into heptane, thus extending potential applications to medium-chain hydrocarbons (i.e., solvent- kerosene-type). The scarcity natural sources presents challenge however. Here, we explore heptane capacity E. coli strain engineered biosynthesize via...
Light is the most abundant source of energy on earth and used by photosynthetic organisms to drive synthesis organic molecules. also allows catalysis few enzymes, photoenzymes. Among them, fatty acid photodecarboxylase (FAP) isolated from microalgae converts acids into hydrocarbons. We present here our understanding role hydrocarbons produced FAP in vivo, catalytic mechanism its potential biotechnological applications.