A5085180259- GABA and Rice Research
- Plant biochemistry and biosynthesis
- Metabolomics and Mass Spectrometry Studies
- Plant Surface Properties and Treatments
- Lipid metabolism and biosynthesis
- Plant Gene Expression Analysis
- Retinal Development and Disorders
- Medicinal Plants and Neuroprotection
- Biochemical and biochemical processes
- Postharvest Quality and Shelf Life Management
- Plant Genetic and Mutation Studies
- Microbial Community Ecology and Physiology
- Algal biology and biofuel production
- Circadian rhythm and melatonin
- Isotope Analysis in Ecology
- Plant Physiology and Cultivation Studies
- Food Chemistry and Fat Analysis
- Ginseng Biological Effects and Applications
- Diatoms and Algae Research
- Marine and coastal ecosystems
- Biodiesel Production and Applications
- Photoreceptor and optogenetics research
- Tea Polyphenols and Effects
- Genetic Mapping and Diversity in Plants and Animals
- Photosynthetic Processes and Mechanisms
Purdue University West Lafayette
2017-2024
Michigan State University
2012-2017
University of Guelph
2008-2017
Purdue University System
2017
Great Lakes Bioenergy Research Center
2016
United States Department of Energy
2014
KBase
2014
Michigan United
2012
Unicellular marine algae have promise for providing sustainable and scalable biofuel feedstocks, although no single species has emerged as a preferred organism. Moreover, adequate molecular genetic resources prerequisite the rational engineering of algal feedstocks are lacking most candidate species. Heterokonts genus Nannochloropsis naturally high cellular oil content already in use industrial production high-value lipid products. First success applying reverse genetics by targeted gene...
Abstract Arabidopsis (Arabidopsis thaliana) has eight glycerol-3-phosphate acyltransferase (GPAT) genes that are members of a plant-specific family with three distinct clades. Several these GPATs required for the synthesis cutin or suberin. Unlike sn-1 regiospecificity involved in membrane storage lipid synthesis, GPAT4 and -6 unique bifunctional enzymes both sn-2 phosphatase activity resulting 2-monoacylglycerol products. We present enzymology, pathway organization, evolutionary analysis...
γ-Aminobutyrate (GABA) is a ubiquitous nonprotein amino acid that accumulates in plants response to abiotic and biotic stresses. In companion paper, we discussed the origin of GABA from glutamate subsequent catabolism succinic semialdehyde either succinate or γ-hydroxybutyrate (GHB), characteristics genes proteins responsible for permease, decarboxylase, transaminase, dehydrogenase, reductase activities. this explore gene expression transcript–metabolite relationships during stress, describe...
A diacylglycerol acetyltransferase, EaDAcT, from Euonymus alatus, synthesizes sn-3 acetyl triacylglycerols (acetyl-TAG) when expressed in Arabidopsis, Camelina and soybean. Compared to most vegetable oils, acetyl-TAGs have reduced viscosity improved cold temperature properties that confer advantages applications as biodegradable lubricants, food emulsifiers, plasticizers, 'drop-in' fuels for some diesel engines. high-oleic line was engineered express the EaDAcT gene order produce acetyl-TAG...
Enzymes that reduce the aldehyde chemical grouping (i.e. H-C=O) to its corresponding alcohol could be crucial in maintaining plant health. Recently, recombinant expression of a cytosolic enzyme from Arabidopsis thaliana (L.) Heynh (designated as glyoxylate reductase 1 or AtGR1) revealed it effectively catalyses vitro reduction both and succinic semialdehyde (SSA). In this paper, web-based bioinformatics tools second putative GR cDNA (GenBank Accession No. AAP42747; designated herein AtGR2)...
Lipid peroxides are generated by oxidative stress in cells, and contribute to ageing neurodegenerative disease. The eye is at special risk for lipid peroxidation because photoreceptors possess amplified sensory membranes rich peroxidation-susceptible polyunsaturated fatty acids. Light-induced the retina contributes retinal degeneration, has been implicated progression of age-associated ocular diseases such as age-related macular degeneration (AMD). Here, we show that exposing Drosophila...
The synthesis of small organic molecules, known as specialized or secondary metabolites, is one mechanism by which plants resist and tolerate biotic abiotic stress. Many metabolites are derived from the aromatic amino acids phenylalanine (Phe) tyrosine (Tyr). In addition, improved characterization compounds these could inform strategies for developing crops with greater resilience traits biorefinery. Sorghum other grasses possess ammonia-lyase (PAL) enzymes that generate cinnamic acid Phe...
Bayberry (Myrica pensylvanica) fruits synthesize an extremely thick and unusual layer of crystalline surface wax that accumulates to 32% fruit dry weight, the highest reported lipid accumulation in plants. The composition is also striking, consisting completely saturated triacylglycerol, diacylglycerol, monoacylglycerol with palmitate myristate acyl chains. To gain insight into unique properties synthesis, we examined chemical morphological development layer, monitored biosynthesis through...
Plant NADPH-dependent glyoxylate/succinic semialdehyde reductases 1 and 2 (cytosolic GLYR1 plastidial/mitochondrial GLYR2) are considered to be of particular importance under abiotic stress conditions. Here, the apple (Malus x domestica Borkh.) rice (Oryza sativa L.) GLYR1s GLYR2s were characterized their kinetic properties compared those previously GLYRs from Arabidopsis thaliana [L.] Heynh. The purified recombinant had an affinity for glyoxylate succinic semialdehyde, respectively, in low...
Succinate semialdehyde (SSA) is a mitochondrially generated intermediate in the metabolism of γ-aminobutyrate (GABA), which accumulates response to variety biotic and abiotic stresses. SSA can be reduced γ-hydroxybutyrate (GHB) plants exposed various stress conditions. Recent evidence indicates that distinct cytosolic plastidial glyoxylate reductase isoforms from Arabidopsis thaliana (L.) Heynh (GLYR1 GLYR2, respectively) catalyze vitro conversion GHB, as well glycolate, via NADPH-dependent...
The optimal extraction of information from untargeted metabolomics analyses is a continuing challenge. Here, we describe an approach that combines stable isotope labeling, liquid chromatography- mass spectrometry (LC-MS), and computational pipeline to automatically identify metabolites produced selected metabolic precursor. We identified the subset soluble metabolome generated phenylalanine (Phe) in Arabidopsis thaliana, which refer as Phe-derived (FDM) In addition identifying present single...
We tested the hypothesis that γ-aminobutyrate transaminase (GABA-T) regulates supply of succinate semialdehyde for dehydrogenase or NADPH-dependent glyoxylate/succinate reductase 1 (GLYR1) during stress. Constitutive overexpression (OX) lines GABA-T were generated in Arabidopsis via floral-dip method Agrobacterium-mediated transformation. Polymerase chain reaction enabled selection four transgenic with higher transcript levels than wild-type (WT), but assay cell-free leaf extracts revealed...
Bayberry (Myrica pensylvanica) fruits are covered with a remarkably thick layer of crystalline wax consisting triacylglycerol (TAG) and diacylglycerol (DAG) esterified exclusively saturated fatty acids. As the only plant known to accumulate soluble glycerolipids as major component surface waxes, represents novel system investigate neutral lipid biosynthesis secretion by vegetative cells. The assembly is distinct from conventional TAG other instead proceeds through pathway related cutin...
SUMMARY Genome‐wide association studies (GWAS) are an effective approach to identify new specialized metabolites and the genes involved in their biosynthesis regulation. In this study, GWAS of Arabidopsis thaliana soluble leaf stem identified alleles uncharacterized BAHD‐family acyltransferase (AT5G57840) associated with natural variation three structurally related metabolites. These were esters glucuronosylglycerol, one metabolite containing phenylacetic acid as acyl component ester....
Untargeted metabolomics is a well-known and reliable method to globally profile small molecules. A number of analytical tools can be used accomplish this goal, but liquid chromatography mass spectrometry (LC/MS) most often due its precision sensitivity. Although the use LC/MS results in large amounts data, much it difficult time consuming analyze. Most importantly, cannot conclusively identify molecules; additional are needed address these limitations. Effort has been put into streamlining...
Abstract ISOCHORISMATE SYNTHASE (ICS) catalyzes the isomerization of chorismate to isochorismate, an essential precursor in biosynthesis Photosystem I electron carrier phylloquinone and one two pathways for defense response hormone salicylic acid (SA). We characterized a Zea mays ics1 mutant impacts on metabolism, photosynthesis, immune signaling. Phylloquinone was reduced resulting low transfer rates high backflow rates. SA accumulation induced by autoactive alleles nucleotide-binding...